Restriction map for
pF1KA1521
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# NO CUT ENZYMES ( 73 enzymes) #
##################################
Acc65I AgeI AhdI Aor51HI ApaI
AscI Asp718I AspEI AviII BamHI
BbeI BbrPI BciVI BclI BglI
BlpI Bpu1102I BsaAI BsiEI BsiWI
BsmBI BssHII BssSI Bst1107I BstEII
BstPI BstZ17I CelII CpoI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I EcoRV EcoT22I FbaI FseI
FspI HaeII HpaI KasI KpnI
KspI MluI NaeI NarI NgoMIV
NotI NsiI PacI PaeR7I PinAI
PmaCI PmeI PmlI PshAI PvuI
RsrII SacII SbfI ScaI SfiI
SfoI SgrAI SnaBI SphI Sse8387I
SwaI XbaI XhoI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AatII 1972 GACGTC GACGT^C
AccIII 2726 TCCGGA T^CCGGA
AclI 2939 AACGTT AA^CGTT
AflII 103 CTTAAG C^TTAAG
AflIII 2048 ACATGT A^C[GA][CT]GT
AocI 1553 CCTTAGG CC^T[ACGT]AGG
AspI 4240 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 499 CCTAGG C^CTAGG
BanI 2428 GGTGCC G^G[CT][GA]CC
BcgI 3837 CGATAAAACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 103 CTTAAG C^TTAAG
BglII 2356 AGATCT A^GATCT
BlnI 499 CCTAGG C^CTAGG
BseAI 2726 TCCGGA T^CCGGA
Bsp1407I 860 TGTACA T^GTACA
BspEI 2726 TCCGGA T^CCGGA
BspLU11I 2048 ACATGT A^CATGT
BsrBI 4253 GAGCGG CCGCTC(-3/-3)
BsrFI 2756 ACCGGC [GA]^CCGG[CT]
BsrGI 860 TGTACA T^GTACA
BstBI 2693 TTCGAA TT^CGAA
BstXI 221 CCAAAATTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1553 CCTTAGG CC^T[ACGT]AGG
Cfr10I 2756 ACCGGC [GA]^CCGG[CT]
DraI 544 TTTAAA TTT^AAA
DraIII 374 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 1553 CCTTAGG CC^T[ACGT]AGG
HincII 3659 GTCGAC GT[CT]^[GA]AC
HindII 3659 GTCGAC GT[CT]^[GA]AC
MroI 2726 TCCGGA T^CCGGA
MslI 1461 CACCAATATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NheI 4348 GCTAGC G^CTAGC
NruI 4017 TCGCGA TCG^CGA
NspV 2693 TTCGAA TT^CGAA
PflFI 4240 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 2614 CCAAACTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I 2939 AACGTT AA^CGTT
SalI 3659 GTCGAC G^TCGAC
SexAI 4022 ACCAGGT A^CC[AT]GGT
SfuI 2693 TTCGAA TT^CGAA
SmaI 4169 CCCGGG CCC^GGG
SspBI 860 TGTACA T^GTACA
SspI 1999 AATATT AAT^ATT
StuI 2289 AGGCCT AGG^CCT
Tth111I 4240 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 2614 CCAAACTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1463 CCAATATGCTAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 4169 CCCGGG C^CCGGG
XmaI 4169 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AccI 3659 GTCGAC GT^[AC][GT]AC
4317 GTCTAC
AccII 3912 CGCG CG^CG
4018 CGCG
AcyI 1972 GACGTC G[GA]^CG[CT]C
2759 GGCGTC
Alw44I 3068 GTGCAC G^TGCAC
3245 GTGCAC
AlwNI 2030 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG
3302 CAGACTCTG
ApaLI 3068 GTGCAC G^TGCAC
3245 GTGCAC
AseI 997 ATTAAT AT^TAAT
3508 ATTAAT
AsnI 997 ATTAAT AT^TAAT
3508 ATTAAT
AvaI 2850 CCCGAG C^[CT]CG[GA]G
4169 CCCGGG
BsaHI 1972 GACGTC G[GA]^CG[CT]C
2759 GGCGTC
BsaWI 2726 TCCGGA [AT]^CCGG[AT]
3968 ACCGGA
BsoBI 2850 CCCGAG C^[CT]CG[GA]G
4169 CCCGGG
BspHI 3177 TCATGA T^CATGA
4032 TCATGA
BspMI 1026 GCAGGT ACCTGC(4/8)
2029 GCAGGT
BstAPI 133 GCATGGATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2029 GCAGGTGCTGC
BstUI 3912 CGCG CG^CG
4018 CGCG
CfoI 1689 GCGC GCG^C
3555 GCGC
DsaI 1665 CCATGG C^C[GA][CT]GG
4117 CCATGG
HgaI 2199 GCGTC GACGC(5/10)
2760 GCGTC
HhaI 1689 GCGC GCG^C
3555 GCGC
Hin1I 1972 GACGTC G[GA]^CG[CT]C
2759 GGCGTC
HinP1I 1689 GCGC G^CGC
3555 GCGC
HindIII 191 AAGCTT A^AGCTT
2138 AAGCTT
MaeII 1973 ACGT A^CGT
2940 ACGT
MfeI 262 CAATTG C^AATTG
414 CAATTG
MunI 262 CAATTG C^AATTG
414 CAATTG
MvnI 3912 CGCG CG^CG
4018 CGCG
NcoI 1665 CCATGG C^CATGG
4117 CCATGG
NdeI 1483 CATATG CA^TATG
3900 CATATG
PshBI 997 ATTAAT AT^TAAT
3508 ATTAAT
RcaI 3177 TCATGA T^CATGA
4032 TCATGA
SacI 2703 GAGCTC GAGCT^C
4079 GAGCTC
SapI 2773 GAAGAGC GCTCTTC(1/4)
4081 GCTCTTC
SpeI 2398 ACTAGT A^CTAGT
2688 ACTAGT
#########################################
# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI Asp700I AspHI AvaII BalI
BanII BbsI BbvCI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaBI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I BspDI BsrDI BsrI BssKI
BstF5I BstNI BstYI Cac8I Cfr13I
ClaI DdeI DpnII DraII EaeI
EarI EcoNI EcoO109I EcoRI EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MamI MboI
MboII MflI MluNI MnlI MscI
MseI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII XmnI