Restriction map for
pF1KA1496
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# NO CUT ENZYMES ( 108 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AflII AgeI Alw44I Aor51HI
ApaLI AscI Asp718I AspI AviII
AvrII BalI BbeI BbrPI BbvCI
BcgI BfrI BglI BglII BlnI
BsaAI BsaBI BsaI BsaWI BseAI
BsiEI BsiWI Bsp1407I BspEI BspHI
BsrBI BsrGI BssHII Bst1107I BstAPI
BstBI BstUI BstXI BstZ17I CpoI
DraIII DrdI EaeI EagI EclXI
Eco47III Eco52I EcoRV FseI FspI
HincII HindII HindIII HpaI KasI
KpnI KspI MamI MluI MluNI
MroI MscI MvnI NarI NheI
NotI NruI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI Psp1406I PvuI RcaI
RsrII SacII SalI SbfI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tth111I XbaI XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AcyI 929 GGCGTC G[GA]^CG[CT]C
AflIII 583 ACATGT A^C[GA][CT]GT
AhdI 2829 GACTAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1948 CCTGAGG CC^T[ACGT]AGG
ApaI 1616 GGGCCC GGGCC^C
Asp700I 703 GAAACTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 2829 GACTAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 1198 GAGCTC G[AT]GC[AT]^C
AvaI 614 CCCGAG C^[CT]CG[GA]G
BbsI 1965 GAAGAC GAAGAC(2/6)
BciVI 279 GGATAC GTATCC(6/5)
BclI 1688 TGATCA T^GATCA
BcnI 2637 CCGGG CC^[GC]GG
BlpI 1195 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1195 GCTGAGC GC^T[ACGT]AGC
BpuAI 1965 GAAGAC GAAGAC(2/6)
BsaHI 929 GGCGTC G[GA]^CG[CT]C
BsiHKAI 1198 GAGCTC G[AT]GC[AT]^C
BsmBI 931 CGTCTC CGTCTC(1/5)
BsoBI 614 CCCGAG C^[CT]CG[GA]G
BspDI 1957 ATCGAT AT^CGAT
BspLU11I 583 ACATGT A^CATGT
BssSI 905 CACGAG CACGAG(-5/-1)
BstEII 2232 GGTTACC G^GT[ACGT]ACC
BstPI 2232 GGTTACC G^GT[ACGT]ACC
Bsu36I 1948 CCTGAGG CC^T[ACGT]AGG
CelII 1195 GCTGAGC GC^T[ACGT]AGC
ClaI 1957 ATCGAT AT^CGAT
DraI 370 TTTAAA TTT^AAA
Eam1105I 2829 GACTAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1948 CCTGAGG CC^T[ACGT]AGG
EcoNI 1304 CCTCCCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 2232 GGTTACC G^GT[ACGT]ACC
EcoRI 900 GAATTC G^AATTC
EcoT22I 888 ATGCAT ATGCA^T
FauI 1578 CCCGC CCCGC(4/6)
FbaI 1688 TGATCA T^GATCA
Hin1I 929 GGCGTC G[GA]^CG[CT]C
MfeI 323 CAATTG C^AATTG
MunI 323 CAATTG C^AATTG
NaeI 2653 GCCGGC GCC^GGC
NciI 2637 CCGGG CC^[GC]GG
NgoMIV 2653 GCCGGC G^CCGGC
NsiI 888 ATGCAT ATGCA^T
PstI 2390 CTGCAG CTGCA^G
SacI 1198 GAGCTC GAGCT^C
SexAI 1600 ACCTGGT A^CC[AT]GGT
SmlI 2420 CTCAAG C^T[CT][GA]AG
XmnI 703 GAAACTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 21 enzymes) #
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AseI 259 ATTAAT AT^TAAT
775 ATTAAT
AsnI 259 ATTAAT AT^TAAT
775 ATTAAT
BfaI 1078 CTAG C^TAG
2831 CTAG
BpmI 1220 CTCCAG CTGGAG(16/14)
449 CTGGAG
BsgI 1261 GTGCAG GTGCAG(16/14)
1348 GTGCAG
BsmFI 2947 GGGAC GGGAC(10/14)
2170 GTCCC
BsrDI 891 CATTGC GCAATG(2/0)
2492 GCAATG
BsrFI 2138 GCCGGT [GA]^CCGG[CT]
2653 GCCGGC
CfoI 2657 GCGC GCG^C
2672 GCGC
Cfr10I 2138 GCCGGT [GA]^CCGG[CT]
2653 GCCGGC
HaeII 2656 GGCGCT [GA]GCGC^[CT]
2671 AGCGCC
HhaI 2657 GCGC GCG^C
2672 GCGC
HinP1I 2657 GCGC G^CGC
2672 GCGC
MaeI 1078 CTAG C^TAG
2831 CTAG
MaeII 648 ACGT A^CGT
2846 ACGT
NdeI 461 CATATG CA^TATG
2318 CATATG
NspI 583 ACATGT [GA]CATG^[CT]
1145 GCATGT
PleI 1388 GACTC GAGTC(4/5)
2794 GAGTC
PshBI 259 ATTAAT AT^TAAT
775 ATTAAT
SapI 2398 GAAGAGC GCTCTTC(1/4)
2607 GAAGAGC
ScaI 2853 AGTACT AGT^ACT
3054 AGTACT
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# 3 OR MORE CUT ENZYMES ( 80 enzymes) #
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AciI AcsI AfaI AluI AlwI
AlwNI ApoI AvaII BamHI BanI
BanII BbvI BmyI BsaJI BseRI
BsiYI BslI BsmAI BsmI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
DraII DsaI EarI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HgaI HinfI HpaII HphI ItaI
Ksp632I MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NcoI NdeII NlaIII
NlaIV PflMI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoII