################################## # NO CUT ENZYMES ( 115 enzymes) # ################################## AatII AccIII AclI AcyI AflII AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI Asp700I AspEI AvaI AvrII BalI BbeI BbrPI BcgI BclI BcnI BfrI BglI BlnI BlpI Bpu1102I BsaHI BsaWI BseAI BsgI BsiEI BsiWI BsoBI BspDI BspEI BsrBI BsrFI BssHII BssSI BstAPI BstBI CelII Cfr10I ClaI CpoI DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I EcoRI EcoRV FbaI FseI HaeII HapII HgaI Hin1I HincII HindII HpaI HpaII KasI MfeI MluI MluNI MroI MscI MspI MunI NaeI NarI NciI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PshAI Psp1406I PstI PvuI RsrII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I StuI SwaI Van91I XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 57 enzymes) # ################################### Acc65I 759 GGTACC G^GTACC AflIII 282 ACATGT A^C[GA][CT]GT AocI 826 CCTAAGG CC^T[ACGT]AGG Asp718I 759 GGTACC G^GTACC AspI 508 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 2 TGCGCA TGC^GCA BamHI 1990 GGATCC G^GATCC BanI 759 GGTACC G^G[CT][GA]CC BbsI 193 GAAGAC GAAGAC(2/6) BbvCI 2030 CCTCAGC CCTCAGC(-5/-2) BglII 1259 AGATCT A^GATCT BpuAI 193 GAAGAC GAAGAC(2/6) BsaAI 1074 TACGTG [CT]AC^GT[GA] BsaBI 1254 GATAAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 1027 GAGACC GGTCTC(1/5) BseRI 1096 GAGGAG GAGGAG(10/8) BsmBI 1115 GAGACG CGTCTC(1/5) BsmI 687 GCATTC GAATGC(1/-1) BspHI 1388 TCATGA T^CATGA BspLU11I 282 ACATGT A^CATGT BsrDI 608 CATTGC GCAATG(2/0) Bst1107I 435 GTATAC GTA^TAC BstEII 710 GGTCACC G^GT[ACGT]ACC BstPI 710 GGTCACC G^GT[ACGT]ACC BstXI 968 CCAAGACTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstZ17I 435 GTATAC GTA^TAC Bsu36I 826 CCTAAGG CC^T[ACGT]AGG Cac8I 1697 GCCAGC GC[ACGT]^[ACGT]GC CfoI 3 GCGC GCG^C DraII 1469 GGGTCCC [GA]G^G[ACGT]CC[CT] Eco81I 826 CCTAAGG CC^T[ACGT]AGG EcoNI 826 CCTAAGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 1469 GGGTCCC [GA]G^G[ACGT]CC[CT] EcoO65I 710 GGTCACC G^GT[ACGT]ACC EcoT22I 1371 ATGCAT ATGCA^T FauI 20 GCGGG CCCGC(4/6) FspI 2 TGCGCA TGC^GCA HhaI 3 GCGC GCG^C HinP1I 3 GCGC G^CGC HindIII 1975 AAGCTT A^AGCTT KpnI 759 GGTACC GGTAC^C KspI 18 CCGCGG CCGC^GG MamI 1254 GATAAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NcoI 767 CCATGG C^CATGG NsiI 1371 ATGCAT ATGCA^T NspI 282 ACATGT [GA]CATG^[CT] PflFI 508 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC PpuMI 1469 GGGTCCC [GA]G^G[AT]CC[CT] RcaI 1388 TCATGA T^CATGA SacI 898 GAGCTC GAGCT^C SacII 18 CCGCGG CCGC^GG SspI 1511 AATATT AAT^ATT TaqI 1827 TCGA T^CGA Tsp45I 711 GTCAC ^GT[GC]AC Tth111I 508 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 1827 TCGA T^CGA XbaI 1111 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AccI 435 GTATAC GT^[AC][GT]AC 2236 GTCTAC AccII 19 CGCG CG^CG 525 CGCG AseI 2136 ATTAAT AT^TAAT 2190 ATTAAT AsnI 2136 ATTAAT AT^TAAT 2190 ATTAAT AspHI 898 GAGCTC G[AT]GC[AT]^C 1302 GAGCAC BanII 898 GAGCTC G[GA]GC[CT]^C 1099 GAGCCC BfaI 470 CTAG C^TAG 1112 CTAG BsiHKAI 898 GAGCTC G[AT]GC[AT]^C 1302 GAGCAC BsmAI 1027 GAGAC GTCTC(1/5) 1115 GAGAC Bsp1407I 1334 TGTACA T^GTACA 1402 TGTACA BspMI 832 GCAGGT ACCTGC(4/8) 2232 GCAGGT BsrGI 1334 TGTACA T^GTACA 1402 TGTACA BstUI 19 CGCG CG^CG 525 CGCG BstYI 1259 AGATCT [GA]^GATC[CT] 1990 GGATCC HaeIII 528 GGCC GG^CC 1696 GGCC MaeI 470 CTAG C^TAG 1112 CTAG MflI 1259 AGATCT [GA]^GATC[CT] 1990 GGATCC MvnI 19 CGCG CG^CG 525 CGCG NdeI 404 CATATG CA^TATG 446 CATATG PshBI 2136 ATTAAT AT^TAAT 2190 ATTAAT SapI 2268 GAAGAGC GCTCTTC(1/4) 1663 GCTCTTC SfcI 1034 CTACAG C^T[GA][CT]AG 2286 CTACAG SspBI 1334 TGTACA T^GTACA 1402 TGTACA XhoII 1259 AGATCT [GA]^GATC[CT] 1990 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 62 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI AlwNI ApoI AvaII BbvI BciVI BmyI BpmI BsaJI BsiYI BslI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI Cfr13I DdeI DpnII DraI DsaI EarI EcoRII EcoT14I Fnu4HI FokI HinfI HphI ItaI Ksp632I MaeII MaeIII MboI MboII MnlI MseI MslI MspA1I MvaI MwoI NdeII NlaIII NlaIV PleI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI StyI TfiI Tru9I TseI Tsp509I TspRI XcmI