Restriction map for pF1KA1492
##################################
# NO CUT ENZYMES (  115 enzymes) #
##################################

AatII       AccIII      AclI        AcyI        AflII       
AgeI        AhdI        Alw44I      Aor51HI     ApaI        
ApaLI       AscI        Asp700I     AspEI       AvaI        
AvrII       BalI        BbeI        BbrPI       BcgI        
BclI        BcnI        BfrI        BglI        BlnI        
BlpI        Bpu1102I    BsaHI       BsaWI       BseAI       
BsgI        BsiEI       BsiWI       BsoBI       BspDI       
BspEI       BsrBI       BsrFI       BssHII      BssSI       
BstAPI      BstBI       CelII       Cfr10I      ClaI        
CpoI        DraIII      DrdI        EaeI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      EcoRI       
EcoRV       FbaI        FseI        HaeII       HapII       
HgaI        Hin1I       HincII      HindII      HpaI        
HpaII       KasI        MfeI        MluI        MluNI       
MroI        MscI        MspI        MunI        NaeI        
NarI        NciI        NgoMIV      NheI        NotI        
NruI        NspV        PacI        PaeR7I      PflMI       
PinAI       PmaCI       PmeI        PmlI        PshAI       
Psp1406I    PstI        PvuI        RsrII       SalI        
SbfI        ScaI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    StuI        SwaI        
Van91I      XhoI        XmaCI       XmaI        XmnI        


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# ONE CUT ENZYMES (   57 enzymes) #
###################################

Acc65I        759  GGTACC               G^GTACC
AflIII        282  ACATGT               A^C[GA][CT]GT
AocI          826  CCTAAGG              CC^T[ACGT]AGG
Asp718I       759  GGTACC               G^GTACC
AspI          508  GACTCCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII           2  TGCGCA               TGC^GCA
BamHI        1990  GGATCC               G^GATCC
BanI          759  GGTACC               G^G[CT][GA]CC
BbsI          193  GAAGAC               GAAGAC(2/6)
BbvCI        2030  CCTCAGC              CCTCAGC(-5/-2)
BglII        1259  AGATCT               A^GATCT
BpuAI         193  GAAGAC               GAAGAC(2/6)
BsaAI        1074  TACGTG               [CT]AC^GT[GA]
BsaBI        1254  GATAAAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI         1027  GAGACC               GGTCTC(1/5)
BseRI        1096  GAGGAG               GAGGAG(10/8)
BsmBI        1115  GAGACG               CGTCTC(1/5)
BsmI          687  GCATTC               GAATGC(1/-1)
BspHI        1388  TCATGA               T^CATGA
BspLU11I      282  ACATGT               A^CATGT
BsrDI         608  CATTGC               GCAATG(2/0)
Bst1107I      435  GTATAC               GTA^TAC
BstEII        710  GGTCACC              G^GT[ACGT]ACC
BstPI         710  GGTCACC              G^GT[ACGT]ACC
BstXI         968  CCAAGACTGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I       435  GTATAC               GTA^TAC
Bsu36I        826  CCTAAGG              CC^T[ACGT]AGG
Cac8I        1697  GCCAGC               GC[ACGT]^[ACGT]GC
CfoI            3  GCGC                 GCG^C
DraII        1469  GGGTCCC              [GA]G^G[ACGT]CC[CT]
Eco81I        826  CCTAAGG              CC^T[ACGT]AGG
EcoNI         826  CCTAAGGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I     1469  GGGTCCC              [GA]G^G[ACGT]CC[CT]
EcoO65I       710  GGTCACC              G^GT[ACGT]ACC
EcoT22I      1371  ATGCAT               ATGCA^T
FauI           20  GCGGG                CCCGC(4/6)
FspI            2  TGCGCA               TGC^GCA
HhaI            3  GCGC                 GCG^C
HinP1I          3  GCGC                 G^CGC
HindIII      1975  AAGCTT               A^AGCTT
KpnI          759  GGTACC               GGTAC^C
KspI           18  CCGCGG               CCGC^GG
MamI         1254  GATAAAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NcoI          767  CCATGG               C^CATGG
NsiI         1371  ATGCAT               ATGCA^T
NspI          282  ACATGT               [GA]CATG^[CT]
PflFI         508  GACTCCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI        1469  GGGTCCC              [GA]G^G[AT]CC[CT]
RcaI         1388  TCATGA               T^CATGA
SacI          898  GAGCTC               GAGCT^C
SacII          18  CCGCGG               CCGC^GG
SspI         1511  AATATT               AAT^ATT
TaqI         1827  TCGA                 T^CGA
Tsp45I        711  GTCAC                ^GT[GC]AC
Tth111I       508  GACTCCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I      1827  TCGA                 T^CGA
XbaI         1111  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AccI          435  GTATAC               GT^[AC][GT]AC
             2236  GTCTAC              
AccII          19  CGCG                 CG^CG
              525  CGCG                
AseI         2136  ATTAAT               AT^TAAT
             2190  ATTAAT              
AsnI         2136  ATTAAT               AT^TAAT
             2190  ATTAAT              
AspHI         898  GAGCTC               G[AT]GC[AT]^C
             1302  GAGCAC              
BanII         898  GAGCTC               G[GA]GC[CT]^C
             1099  GAGCCC              
BfaI          470  CTAG                 C^TAG
             1112  CTAG                
BsiHKAI       898  GAGCTC               G[AT]GC[AT]^C
             1302  GAGCAC              
BsmAI        1027  GAGAC                GTCTC(1/5)
             1115  GAGAC               
Bsp1407I     1334  TGTACA               T^GTACA
             1402  TGTACA              
BspMI         832  GCAGGT               ACCTGC(4/8)
             2232  GCAGGT              
BsrGI        1334  TGTACA               T^GTACA
             1402  TGTACA              
BstUI          19  CGCG                 CG^CG
              525  CGCG                
BstYI        1259  AGATCT               [GA]^GATC[CT]
             1990  GGATCC              
HaeIII        528  GGCC                 GG^CC
             1696  GGCC                
MaeI          470  CTAG                 C^TAG
             1112  CTAG                
MflI         1259  AGATCT               [GA]^GATC[CT]
             1990  GGATCC              
MvnI           19  CGCG                 CG^CG
              525  CGCG                
NdeI          404  CATATG               CA^TATG
              446  CATATG              
PshBI        2136  ATTAAT               AT^TAAT
             2190  ATTAAT              
SapI         2268  GAAGAGC              GCTCTTC(1/4)
             1663  GCTCTTC             
SfcI         1034  CTACAG               C^T[GA][CT]AG
             2286  CTACAG              
SspBI        1334  TGTACA               T^GTACA
             1402  TGTACA              
XhoII        1259  AGATCT               [GA]^GATC[CT]
             1990  GGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   62 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
AlwNI       ApoI        AvaII       BbvI        BciVI       
BmyI        BpmI        BsaJI       BsiYI       BslI        
BsmFI       Bsp1286I    BsrI        BssKI       BstF5I      
BstNI       Cfr13I      DdeI        DpnII       DraI        
DsaI        EarI        EcoRII      EcoT14I     Fnu4HI      
FokI        HinfI       HphI        ItaI        Ksp632I     
MaeII       MaeIII      MboI        MboII       MnlI        
MseI        MslI        MspA1I      MvaI        MwoI        
NdeII       NlaIII      NlaIV       PleI        PvuII       
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
StyI        TfiI        Tru9I       TseI        Tsp509I     
TspRI       XcmI        
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