Restriction map for
pF1KA1472
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# NO CUT ENZYMES ( 109 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
Alw44I Aor51HI ApaLI AscI AseI
AsnI Asp700I Asp718I AspI AviII
AvrII BalI BbeI BbrPI BbvCI
BcgI BciVI BclI BglII BlnI
BsaAI BsaBI BsaWI BseAI BsiEI
BsiWI BsmBI Bsp1407I BspDI BspEI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstBI BstZ17I Cfr10I ClaI
CpoI DraI DrdI EaeI EagI
EclXI Eco47III Eco52I EcoRV EcoT14I
EcoT22I FbaI FseI FspI HindIII
HpaI KasI KpnI MamI MfeI
MluI MluNI MroI MscI MunI
NaeI NarI NcoI NgoMIV NheI
NotI NruI NsiI NspV PacI
PflFI PflMI PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RsrII
SbfI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SspBI
StyI SwaI Tth111I Van91I XbaI
XcmI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AccI 492 GTCGAC GT^[AC][GT]AC
AcsI 71 GAATTC [GA]^AATT[CT]
AflII 904 CTTAAG C^TTAAG
AflIII 749 ACATGT A^C[GA][CT]GT
AhdI 607 GACCTTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 478 CCTGAGG CC^T[ACGT]AGG
ApaI 4 GGGCCC GGGCC^C
ApoI 71 GAATTC [GA]^AATT[CT]
AspEI 607 GACCTTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 1899 GGATCC G^GATCC
BanI 1476 GGTGCC G^G[CT][GA]CC
BfrI 904 CTTAAG C^TTAAG
BsaI 1672 GAGACC GGTCTC(1/5)
BspLU11I 749 ACATGT A^CATGT
BspMI 1254 GCAGGT ACCTGC(4/8)
BsrBI 473 CCGCTC CCGCTC(-3/-3)
BstAPI 30 GCACAGAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1360 GGTGACC G^GT[ACGT]ACC
BstPI 1360 GGTGACC G^GT[ACGT]ACC
BstXI 1415 CCATAGTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 478 CCTGAGG CC^T[ACGT]AGG
Eam1105I 607 GACCTTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 478 CCTGAGG CC^T[ACGT]AGG
EcoNI 1922 CCTTGCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1360 GGTGACC G^GT[ACGT]ACC
EcoRI 71 GAATTC G^AATTC
HincII 492 GTCGAC GT[CT]^[GA]AC
HindII 492 GTCGAC GT[CT]^[GA]AC
KspI 595 CCGCGG CCGC^GG
PshAI 1534 GACCCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 1501 GAGCTC GAGCT^C
SacII 595 CCGCGG CCGC^GG
SalI 492 GTCGAC G^TCGAC
SexAI 423 ACCAGGT A^CC[AT]GGT
SphI 923 GCATGC GCATG^C
SspI 1070 AATATT AAT^ATT
StuI 411 AGGCCT AGG^CCT
Tsp509I 72 AATT ^AATT
###################################
# TWO CUT ENZYMES ( 43 enzymes) #
###################################
AcyI 1554 GGCGTC G[GA]^CG[CT]C
1789 GGCGTC
AspHI 28 GAGCAC G[AT]GC[AT]^C
1501 GAGCTC
AvaI 450 CTCGAG C^[CT]CG[GA]G
1545 CTCGAG
BbsI 270 GAAGAC GAAGAC(2/6)
513 GTCTTC
BcnI 77 CCCGG CC^[GC]GG
957 CCGGG
BfaI 245 CTAG C^TAG
1168 CTAG
BglI 296 GCCCCATTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
967 GCCCAGTTGGC
BlpI 191 GCTCAGC GC^T[ACGT]AGC
636 GCTGAGC
Bpu1102I 191 GCTCAGC GC^T[ACGT]AGC
636 GCTGAGC
BpuAI 270 GAAGAC GAAGAC(2/6)
513 GTCTTC
BsaHI 1554 GGCGTC G[GA]^CG[CT]C
1789 GGCGTC
BsgI 37 GTGCAG GTGCAG(16/14)
624 GTGCAG
BsiHKAI 28 GAGCAC G[AT]GC[AT]^C
1501 GAGCTC
BsoBI 450 CTCGAG C^[CT]CG[GA]G
1545 CTCGAG
BstYI 1827 GGATCT [GA]^GATC[CT]
1899 GGATCC
CelII 191 GCTCAGC GC^T[ACGT]AGC
636 GCTGAGC
CfoI 189 GCGC GCG^C
867 GCGC
DpnII 1828 GATC ^GATC
1900 GATC
HaeII 188 GGCGCT [GA]GCGC^[CT]
866 AGCGCC
HapII 78 CCGG C^CGG
957 CCGG
HgaI 1555 GCGTC GACGC(5/10)
1790 GCGTC
HhaI 189 GCGC GCG^C
867 GCGC
Hin1I 1554 GGCGTC G[GA]^CG[CT]C
1789 GGCGTC
HinP1I 189 GCGC G^CGC
867 GCGC
HpaII 78 CCGG C^CGG
957 CCGG
MaeI 245 CTAG C^TAG
1168 CTAG
MaeII 1397 ACGT A^CGT
1472 ACGT
MboI 1828 GATC ^GATC
1900 GATC
MflI 1827 GGATCT [GA]^GATC[CT]
1899 GGATCC
MslI 1125 CATGGAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1416 CATAGTCATG
MspI 78 CCGG C^CGG
957 CCGG
NciI 77 CCCGG CC^[GC]GG
957 CCGGG
NdeI 97 CATATG CA^TATG
487 CATATG
NdeII 1828 GATC ^GATC
1900 GATC
NspI 749 ACATGT [GA]CATG^[CT]
923 GCATGC
PaeR7I 450 CTCGAG C^TCGAG
1545 CTCGAG
PstI 811 CTGCAG CTGCA^G
1519 CTGCAG
PvuII 634 CAGCTG CAG^CTG
913 CAGCTG
SapI 1194 GAAGAGC GCTCTTC(1/4)
1249 GAAGAGC
Sau3AI 1828 GATC ^GATC
1900 GATC
ScaI 1802 AGTACT AGT^ACT
1879 AGTACT
XhoI 450 CTCGAG C^TCGAG
1545 CTCGAG
XhoII 1827 GGATCT [GA]^GATC[CT]
1899 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AvaII BanII BbvI BmyI
BpmI BsaJI BseRI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I BspHI
BsrI BssKI BstF5I BstNI BstUI
Cac8I Cfr13I DdeI DraII DraIII
DsaI EarI EcoO109I EcoRII FauI
Fnu4HI FokI HaeIII HinfI HphI
ItaI Ksp632I MaeIII MboII MnlI
MseI MspA1I MvaI MvnI MwoI
NlaIII NlaIV PleI PpuMI RcaI
RsaI Sau96I ScrFI SfaNI SfcI
SmlI TaqI TfiI Tru9I TseI
Tsp45I TspRI TthHB8I