Restriction map for
pF1KA1451
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# NO CUT ENZYMES ( 133 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
Asp700I Asp718I AspEI AspHI AspI
AviII BalI BanI BanII BbeI
BbrPI BbvCI BcgI BcnI BglII
BlpI BmyI Bpu1102I BsaHI BsaI
BsaWI BseAI BsgI BsiEI BsiHKAI
BsiWI BsmBI Bsp1286I Bsp1407I BspDI
BspEI BspHI BsrBI BsrGI BssHII
BssSI Bst1107I BstAPI BstEII BstPI
BstUI BstZ17I Bsu36I Cac8I CelII
ClaI CpoI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRV EcoT22I FauI FseI
FspI Hin1I KasI KpnI KspI
MfeI MluI MluNI MroI MscI
MspA1I MunI MvnI NaeI NarI
NciI NdeI NgoMIV NheI NotI
NruI NsiI PacI PflFI PinAI
PmaCI PmeI PmlI PshAI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacI SacII SalI SbfI ScaI
SexAI SfcI SfiI SfoI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI StuI SwaI Tth111I XbaI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AflII 361 CTTAAG C^TTAAG
AseI 2001 ATTAAT AT^TAAT
AsnI 2001 ATTAAT AT^TAAT
AvaI 2072 CTCGAG C^[CT]CG[GA]G
AvrII 1833 CCTAGG C^CTAGG
BamHI 694 GGATCC G^GATCC
BbsI 803 GTCTTC GAAGAC(2/6)
BfrI 361 CTTAAG C^TTAAG
BglI 2355 GCCAACCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 1833 CCTAGG C^CTAGG
BpmI 1753 CTGGAG CTGGAG(16/14)
BpuAI 803 GTCTTC GAAGAC(2/6)
BsaAI 893 TACGTG [CT]AC^GT[GA]
BsaBI 172 GATTTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 2418 CTCCTC GAGGAG(10/8)
BsmI 579 GAATGC GAATGC(1/-1)
BsoBI 2072 CTCGAG C^[CT]CG[GA]G
BspMI 1168 GCAGGT ACCTGC(4/8)
BsrDI 1992 GCAATG GCAATG(2/0)
BsrFI 2577 ACCGGC [GA]^CCGG[CT]
BstBI 2552 TTCGAA TT^CGAA
BstXI 543 CCAAAAAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 135 GCGC GCG^C
Cfr10I 2577 ACCGGC [GA]^CCGG[CT]
DraII 2252 GGGACCC [GA]G^G[ACGT]CC[CT]
EaeI 2579 CGGCCA [CT]^GGCC[GA]
EcoO109I 2252 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoRI 1730 GAATTC G^AATTC
HaeII 134 AGCGCC [GA]GCGC^[CT]
HapII 2578 CCGG C^CGG
HgaI 2333 GCGTC GACGC(5/10)
HhaI 135 GCGC GCG^C
HinP1I 135 GCGC G^CGC
HincII 1668 GTTAAC GT[CT]^[GA]AC
HindII 1668 GTTAAC GT[CT]^[GA]AC
HindIII 149 AAGCTT A^AGCTT
HpaI 1668 GTTAAC GTT^AAC
HpaII 2578 CCGG C^CGG
MamI 172 GATTTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspI 2578 CCGG C^CGG
NcoI 2248 CCATGG C^CATGG
NspV 2552 TTCGAA TT^CGAA
PaeR7I 2072 CTCGAG C^TCGAG
PpuMI 2252 GGGACCC [GA]G^G[AT]CC[CT]
PshBI 2001 ATTAAT AT^TAAT
SapI 2548 GCTCTTC GCTCTTC(1/4)
SfuI 2552 TTCGAA TT^CGAA
XcmI 1149 CCATGAAGAACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 2072 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AciI 688 GCGG CCGC(-3/-1)
1598 GCGG
AflIII 870 ACATGT A^C[GA][CT]GT
1780 ACATGT
BciVI 1795 GGATAC GTATCC(6/5)
1604 GTATCC
BspLU11I 870 ACATGT A^CATGT
1780 ACATGT
BstYI 432 AGATCC [GA]^GATC[CT]
694 GGATCC
DraI 1119 TTTAAA TTT^AAA
1816 TTTAAA
DsaI 2248 CCATGG C^C[GA][CT]GG
2582 CCACGG
EarI 2549 CTCTTC CTCTTC(1/4)
2173 GAAGAG
EcoNI 1257 CCTATCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1344 CCTATCTGAGG
HaeIII 71 GGCC GG^CC
2580 GGCC
Ksp632I 2549 CTCTTC CTCTTC(1/4)
2173 GAAGAG
MflI 432 AGATCC [GA]^GATC[CT]
694 GGATCC
MslI 867 CACACATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
910 CACAGTGGTG
NspI 870 ACATGT [GA]CATG^[CT]
1780 ACATGT
PflMI 494 CCAAGTTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
639 CCATCCTTTGG
SfaNI 772 GATGC GCATC(5/9)
177 GCATC
SmlI 361 CTTAAG C^T[CT][GA]AG
2072 CTCGAG
Van91I 494 CCAAGTTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
639 CCATCCTTTGG
XhoII 432 AGATCC [GA]^GATC[CT]
694 GGATCC
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# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AcsI AfaI AluI AlwI AlwNI
ApoI AvaII BbvI BclI BfaI
BsaJI BsiYI BslI BsmAI BsmFI
BsrI BssKI BstF5I BstNI Cfr13I
DdeI DpnII EcoRII EcoT14I FbaI
Fnu4HI FokI HinfI HphI ItaI
MaeI MaeII MaeIII MboI MboII
MnlI MseI MvaI MwoI NdeII
NlaIII NlaIV PleI RsaI Sau3AI
Sau96I ScrFI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I