Restriction map for
pF1KA1434
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# NO CUT ENZYMES ( 135 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AviII AvrII
BamHI BanI BanII BbeI BbrPI
BbvCI BclI BlnI BlpI Bpu1102I
BsaHI BsaI BsaWI BseAI BsiEI
BsiWI BsmBI BsoBI BspDI BspEI
BspMI BsrBI BsrFI BssHII BssSI
Bst1107I BstEII BstPI BstUI BstZ17I
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DraII DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO109I EcoO65I EcoT14I EcoT22I FauI
FbaI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HindIII HpaI
KasI KpnI KspI MluI MroI
MvnI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI RsrII SacI
SacII SalI SbfI ScaI SexAI
SfcI SfiI SfoI SgrAI SmaI
SnaBI Sse8387I SspI StyI SwaI
Tth111I XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AciI 1938 CCGC CCGC(-3/-1)
AspHI 718 GAGCAC G[AT]GC[AT]^C
BalI 963 TGGCCA TGG^CCA
BbsI 1687 GAAGAC GAAGAC(2/6)
BcgI 627 GCAGCCTGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 898 GGATAC GTATCC(6/5)
BcnI 842 CCCGG CC^[GC]GG
BglI 997 GCCCAGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 1687 GAAGAC GAAGAC(2/6)
BsaAI 721 CACGTA [CT]AC^GT[GA]
BsaBI 1156 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaJI 1106 CCGTGG C^C[ACGT][ACGT]GG
BsiHKAI 718 GAGCAC G[AT]GC[AT]^C
Bsp1407I 1677 TGTACA T^GTACA
BsrGI 1677 TGTACA T^GTACA
BstAPI 1893 GCAGGAATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 700 TTCGAA TT^CGAA
BstXI 548 CCAATAGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraI 1458 TTTAAA TTT^AAA
DsaI 1106 CCGTGG C^C[GA][CT]GG
EaeI 963 TGGCCA [CT]^GGCC[GA]
EcoRI 809 GAATTC G^AATTC
HincII 868 GTTGAC GT[CT]^[GA]AC
HindII 868 GTTGAC GT[CT]^[GA]AC
MamI 1156 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1876 CAATTG C^AATTG
MluNI 963 TGGCCA TGG^CCA
MscI 963 TGGCCA TGG^CCA
MspA1I 1000 CAGCTG C[AC]G^C[GT]G
MunI 1876 CAATTG C^AATTG
NciI 842 CCCGG CC^[GC]GG
NlaIV 666 GGAACC GG[ACGT]^[ACGT]CC
NspV 700 TTCGAA TT^CGAA
PflMI 929 CCAAGTATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 1000 CAGCTG CAG^CTG
SapI 115 GCTCTTC GCTCTTC(1/4)
SfuI 700 TTCGAA TT^CGAA
SpeI 1737 ACTAGT A^CTAGT
SphI 146 GCATGC GCATG^C
SspBI 1677 TGTACA T^GTACA
StuI 492 AGGCCT AGG^CCT
Van91I 929 CCAAGTATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 463 TCTAGA T^CTAGA
XcmI 1386 CCAGCAAAGGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 26 enzymes) #
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AflII 1321 CTTAAG C^TTAAG
1909 CTTAAG
AflIII 382 ACATGT A^C[GA][CT]GT
750 ACATGT
AvaII 241 GGTCC G^G[AT]CC
1608 GGACC
BfrI 1321 CTTAAG C^TTAAG
1909 CTTAAG
BglII 708 AGATCT A^GATCT
1615 AGATCT
BmyI 718 GAGCAC G[AGT]GC[ACT]^C
1102 GTGCCC
BpmI 1213 CTCCAG CTGGAG(16/14)
496 CTGGAG
BseRI 179 GAGGAG GAGGAG(10/8)
1488 GAGGAG
BsmAI 271 GAGAC GTCTC(1/5)
767 GTCTC
BsmFI 1734 GGGAC GGGAC(10/14)
1925 GTCCC
BsmI 2010 GAATGC GAATGC(1/-1)
597 GCATTC
Bsp1286I 718 GAGCAC G[AGT]GC[ACT]^C
1102 GTGCCC
BspHI 827 TCATGA T^CATGA
1116 TCATGA
BspLU11I 382 ACATGT A^CATGT
750 ACATGT
Cfr13I 241 GGTCC G^G[ACGT]CC
1608 GGACC
EcoNI 1086 CCTTTCAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1887 CCTGAAGCAGG
EcoRV 561 GATATC GAT^ATC
1972 GATATC
HaeIII 493 GGCC GG^CC
964 GGCC
HapII 607 CCGG C^CGG
843 CCGG
HpaII 607 CCGG C^CGG
843 CCGG
MspI 607 CCGG C^CGG
843 CCGG
RcaI 827 TCATGA T^CATGA
1116 TCATGA
Sau96I 241 GGTCC G^G[ACGT]CC
1608 GGACC
TaqI 701 TCGA T^CGA
969 TCGA
Tsp45I 908 GTGAC ^GT[GC]AC
1237 GTGAC
TthHB8I 701 TCGA T^CGA
969 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
BbvI BfaI BsgI BsiYI BslI
BsrDI BsrI BssKI BstF5I BstNI
BstYI Cac8I DdeI DpnII EarI
EcoRII Fnu4HI FokI HinfI HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MvaI MwoI NdeII NlaIII
NspI PleI RsaI Sau3AI ScrFI
SfaNI SmlI TfiI Tru9I TseI
Tsp509I TspRI XhoII