Restriction map for
pF1KA1404
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# NO CUT ENZYMES ( 71 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AhdI ApaI AscI Asp718I AspEI
AspI BamHI BbeI BseAI BsiEI
BsiWI BsmBI Bsp1407I BspDI BspEI
BsrBI BsrGI BssHII Bst1107I BstUI
BstZ17I ClaI CpoI DraI DrdI
EagI Eam1105I EclXI Eco52I FseI
HpaI KasI KpnI KspI MluI
MroI MvnI NaeI NarI NdeI
NgoMIV NotI NruI PacI PaeR7I
PflFI PmeI Psp1406I PvuI RsrII
SacII SalI SbfI SfiI SfoI
SmaI SnaBI SpeI Sse8387I SspBI
SwaI Tth111I XbaI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AccI 1279 GTCTAC GT^[AC][GT]AC
AcyI 3532 GGCGTC G[GA]^CG[CT]C
Aor51HI 5231 AGCGCT AGC^GCT
AseI 1744 ATTAAT AT^TAAT
AsnI 1744 ATTAAT AT^TAAT
AvaI 3665 CCCGAG C^[CT]CG[GA]G
AviII 3500 TGCGCA TGC^GCA
AvrII 160 CCTAGG C^CTAGG
BbrPI 5601 CACGTG CAC^GTG
BclI 683 TGATCA T^GATCA
BglII 1425 AGATCT A^GATCT
BlnI 160 CCTAGG C^CTAGG
BlpI 4955 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 4955 GCTCAGC GC^T[ACGT]AGC
BsaAI 5601 CACGTG [CT]AC^GT[GA]
BsaHI 3532 GGCGTC G[GA]^CG[CT]C
BsmI 2334 GCATTC GAATGC(1/-1)
BsoBI 3665 CCCGAG C^[CT]CG[GA]G
BspLU11I 5392 ACATGT A^CATGT
BssSI 5525 CTCGTG CACGAG(-5/-1)
BstAPI 3062 GCAAAGCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 4357 TTCGAA TT^CGAA
CelII 4955 GCTCAGC GC^T[ACGT]AGC
Eco47III 5231 AGCGCT AGC^GCT
EcoRV 3636 GATATC GAT^ATC
FbaI 683 TGATCA T^GATCA
FspI 3500 TGCGCA TGC^GCA
HgaI 3533 GCGTC GACGC(5/10)
Hin1I 3532 GGCGTC G[GA]^CG[CT]C
MfeI 2010 CAATTG C^AATTG
MunI 2010 CAATTG C^AATTG
NheI 1479 GCTAGC G^CTAGC
NspV 4357 TTCGAA TT^CGAA
PmaCI 5601 CACGTG CAC^GTG
PmlI 5601 CACGTG CAC^GTG
PshAI 5092 GACCTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1744 ATTAAT AT^TAAT
ScaI 3425 AGTACT AGT^ACT
SfuI 4357 TTCGAA TT^CGAA
SgrAI 3991 CACCGGTG C[GA]^CCGG[CT]G
SspI 4716 AATATT AAT^ATT
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# TWO CUT ENZYMES ( 23 enzymes) #
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AflII 3292 CTTAAG C^TTAAG
4990 CTTAAG
AgeI 3992 ACCGGT A^CCGGT
4236 ACCGGT
BbvCI 2847 CCTCAGC CCTCAGC(-5/-2)
2088 GCTGAGG
BcgI 1086 CGACAGCACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
4597 CGACCCCCATGC
BcnI 2505 CCGGG CC^[GC]GG
3482 CCGGG
BfrI 3292 CTTAAG C^TTAAG
4990 CTTAAG
BglI 2535 GCCCCAGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
5546 GCCGCAATGGC
BsaI 278 GAGACC GGTCTC(1/5)
4654 GGTCTC
BsaWI 3992 ACCGGT [AT]^CCGG[AT]
4236 ACCGGT
BsrDI 3048 CATTGC GCAATG(2/0)
5549 GCAATG
BsrFI 3992 ACCGGT [GA]^CCGG[CT]
4236 ACCGGT
BstEII 4203 GGTCACC G^GT[ACGT]ACC
4707 GGTCACC
BstPI 4203 GGTCACC G^GT[ACGT]ACC
4707 GGTCACC
Cfr10I 3992 ACCGGT [GA]^CCGG[CT]
4236 ACCGGT
EcoO65I 4203 GGTCACC G^GT[ACGT]ACC
4707 GGTCACC
HincII 853 GTTGAC GT[CT]^[GA]AC
4232 GTCAAC
HindII 853 GTTGAC GT[CT]^[GA]AC
4232 GTCAAC
NciI 2505 CCGGG CC^[GC]GG
3482 CCGGG
PinAI 3992 ACCGGT A^CCGGT
4236 ACCGGT
SacI 514 GAGCTC GAGCT^C
553 GAGCTC
SexAI 307 ACCAGGT A^CC[AT]GGT
5294 ACCTGGT
SphI 258 GCATGC GCATG^C
5691 GCATGC
StuI 1538 AGGCCT AGG^CCT
4260 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 123 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
Alw44I AlwI AlwNI AocI ApaLI
ApoI Asp700I AspHI AvaII BalI
BanI BanII BbsI BbvI BciVI
BfaI BmyI BpmI BpuAI BsaBI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BspHI
BspMI BsrI BssKI BstF5I BstNI
BstXI BstYI Bsu36I Cac8I CfoI
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI EarI Eco81I EcoNI
EcoO109I EcoRI EcoRII EcoT14I EcoT22I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MamI MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NcoI NdeII NlaIII NlaIV NsiI
NspI PflMI PleI PpuMI PstI
PvuII RcaI RsaI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII XmnI