Restriction map for
pF1KA1390
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# NO CUT ENZYMES ( 126 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BanI BbeI
BbrPI BbsI BbvCI BcgI BciVI
BfrI BglI BglII BlnI BpuAI
BsaAI BsaBI BsaWI BseAI BsiEI
BsiWI Bsp1407I BspDI BspEI BspLU11I
BsrBI BsrFI BsrGI BssHII BstBI
BstEII BstPI Cfr10I ClaI CpoI
DraI DrdI EagI Eam1105I EarI
EclXI Eco47III Eco52I EcoO65I EcoRI
EcoRV EcoT22I FseI FspI HaeII
HincII HindII HpaI KasI KpnI
Ksp632I MaeII MamI MfeI MluI
MroI MunI NaeI NarI NdeI
NgoMIV NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RsrII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SspBI
SspI SwaI TaqI Tth111I TthHB8I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AfaI 708 GTAC GT^AC
AvaI 670 CCCGAG C^[CT]CG[GA]G
BalI 611 TGGCCA TGG^CCA
BanII 493 GAGCTC G[GA]GC[CT]^C
BclI 479 TGATCA T^GATCA
BlpI 221 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 221 GCTCAGC GC^T[ACGT]AGC
BsaI 862 GAGACC GGTCTC(1/5)
BsmBI 648 CGTCTC CGTCTC(1/5)
BsmI 1397 GCATTC GAATGC(1/-1)
BsoBI 670 CCCGAG C^[CT]CG[GA]G
BspHI 425 TCATGA T^CATGA
BsrDI 589 GCAATG GCAATG(2/0)
Bst1107I 663 GTATAC GTA^TAC
BstXI 614 CCACAGGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 663 GTATAC GTA^TAC
CelII 221 GCTCAGC GC^T[ACGT]AGC
CfoI 1288 GCGC GCG^C
DraIII 1051 CACAATGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 611 TGGCCA [CT]^GGCC[GA]
FbaI 479 TGATCA T^GATCA
HhaI 1288 GCGC GCG^C
HinP1I 1288 GCGC G^CGC
HindIII 164 AAGCTT A^AGCTT
KspI 869 CCGCGG CCGC^GG
MboII 441 TCTTC GAAGA(8/7)
MluNI 611 TGGCCA TGG^CCA
MscI 611 TGGCCA TGG^CCA
MseI 563 TTAA T^TAA
NcoI 711 CCATGG C^CATGG
NheI 217 GCTAGC G^CTAGC
NspI 277 GCATGC [GA]CATG^[CT]
PshAI 1308 GACGCGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 425 TCATGA T^CATGA
RsaI 708 GTAC GT^AC
SacI 493 GAGCTC GAGCT^C
SacII 869 CCGCGG CCGC^GG
SmlI 196 CTTGAG C^T[CT][GA]AG
SphI 277 GCATGC GCATG^C
StuI 837 AGGCCT AGG^CCT
TfiI 638 GATTC G^A[AT]TC
Tru9I 563 TTAA T^TAA
Tsp509I 565 AATT ^AATT
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# TWO CUT ENZYMES ( 16 enzymes) #
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AccI 663 GTATAC GT^[AC][GT]AC
1165 GTAGAC
AccII 870 CGCG CG^CG
1310 CGCG
AcyI 646 GGCGTC G[GA]^CG[CT]C
1349 GACGCC
AvaII 314 GGACC G^G[AT]CC
1314 GGTCC
BamHI 21 GGATCC G^GATCC
353 GGATCC
BsaHI 646 GGCGTC G[GA]^CG[CT]C
1349 GACGCC
BstAPI 118 GCAAGAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1290 GCAGCCAGTGC
BstUI 870 CGCG CG^CG
1310 CGCG
BstYI 21 GGATCC [GA]^GATC[CT]
353 GGATCC
EcoNI 358 CCTTGGAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1107 CCTGGGCAAGG
Hin1I 646 GGCGTC G[GA]^CG[CT]C
1349 GACGCC
MflI 21 GGATCC [GA]^GATC[CT]
353 GGATCC
MslI 668 CACCCGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
808 CACTCCAGTG
MvnI 870 CGCG CG^CG
1310 CGCG
PpuMI 313 AGGACCC [GA]G^G[AT]CC[CT]
1313 GGGTCCT
XhoII 21 GGATCC [GA]^GATC[CT]
353 GGATCC
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# 3 OR MORE CUT ENZYMES ( 73 enzymes) #
#########################################
AciI AluI AlwI AlwNI AocI
AspHI BbvI BcnI BfaI BmyI
BpmI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrI BssKI BssSI BstF5I
BstNI Bsu36I Cac8I Cfr13I DdeI
DpnII DraII DsaI Eco81I EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HinfI HpaII
HphI ItaI MaeI MaeIII MboI
MnlI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PflMI
PleI PstI PvuII Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TseI
Tsp45I TspRI Van91I