Restriction map for
pF1KA1378
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# NO CUT ENZYMES ( 143 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspHI
AvaI AviII AvrII BalI BanII
BbeI BbrPI BcgI BciVI BcnI
BfrI BglI BglII BlnI BlpI
BmyI Bpu1102I BsaHI BsaI BsaWI
BseAI BsgI BsiEI BsiHKAI BsiWI
BsmBI BsmFI BsoBI Bsp1286I Bsp1407I
BspDI BspEI BspHI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstUI
BstZ17I CelII CfoI ClaI CpoI
DraI DraII DraIII DrdI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoO109I EcoRI EcoRV FauI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HpaI KasI KpnI
KspI MluI MluNI MroI MscI
MspA1I MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacI SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SpeI SphI
Sse8387I SspBI StuI SwaI XbaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AcsI 1814 AAATTC [GA]^AATT[CT]
AflIII 1071 ACATGT A^C[GA][CT]GT
AlwNI 899 CAGTACCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1229 CCTTAGG CC^T[ACGT]AGG
ApoI 1814 AAATTC [GA]^AATT[CT]
Asp700I 1409 GAATGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 403 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 1045 GGACC G^G[AT]CC
BamHI 1834 GGATCC G^GATCC
BanI 1329 GGCACC G^G[CT][GA]CC
BbvCI 724 CCTCAGC CCTCAGC(-5/-2)
BclI 1311 TGATCA T^GATCA
BsaAI 1528 TACGTA [CT]AC^GT[GA]
BsaBI 1441 GATCCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmAI 1761 GTCTC GTCTC(1/5)
BsmI 1009 GAATGC GAATGC(1/-1)
BspLU11I 1071 ACATGT A^CATGT
BsrBI 1573 GAGCGG CCGCTC(-3/-3)
BsrFI 783 GCCGGT [GA]^CCGG[CT]
BssKI 507 CCTGG ^CC[ACGT]GG
BstEII 988 GGTGACC G^GT[ACGT]ACC
BstNI 507 CCTGG CC^[AT]GG
BstPI 988 GGTGACC G^GT[ACGT]ACC
Bsu36I 1229 CCTTAGG CC^T[ACGT]AGG
Cfr10I 783 GCCGGT [GA]^CCGG[CT]
DsaI 1347 CCGTGG C^C[GA][CT]GG
Eco81I 1229 CCTTAGG CC^T[ACGT]AGG
EcoO65I 988 GGTGACC G^GT[ACGT]ACC
EcoRII 507 CCTGG ^CC[AT]GG
EcoT22I 1694 ATGCAT ATGCA^T
FbaI 1311 TGATCA T^GATCA
HaeIII 1237 GGCC GG^CC
HapII 784 CCGG C^CGG
HpaII 784 CCGG C^CGG
MaeII 1529 ACGT A^CGT
MamI 1441 GATCCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspI 784 CCGG C^CGG
MvaI 507 CCTGG CC^[AT]GG
NsiI 1694 ATGCAT ATGCA^T
NspI 1071 ACATGT [GA]CATG^[CT]
PflFI 403 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 701 CCATCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SapI 1487 GAAGAGC GCTCTTC(1/4)
ScrFI 507 CCTGG CC^[ACGT]GG
SnaBI 1528 TACGTA TAC^GTA
SspI 667 AATATT AAT^ATT
Tth111I 403 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 701 CCATCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1628 CCAGAGGTGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 1409 GAATGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AfaI 901 GTAC GT^AC
1322 GTAC
BbsI 355 GAAGAC GAAGAC(2/6)
607 GAAGAC
BpmI 654 CTCCAG CTGGAG(16/14)
1775 CTGGAG
BpuAI 355 GAAGAC GAAGAC(2/6)
607 GAAGAC
BseRI 1556 CTCCTC GAGGAG(10/8)
1352 GAGGAG
BspMI 441 GCAGGT ACCTGC(4/8)
684 GCAGGT
Cfr13I 1045 GGACC G^G[ACGT]CC
1237 GGCCC
EarI 167 CTCTTC CTCTTC(1/4)
1487 GAAGAG
EcoT14I 934 CCAAGG C^C[AT][AT]GG
1220 CCTTGG
HincII 400 GTTGAC GT[CT]^[GA]AC
1849 GTTGAC
HindII 400 GTTGAC GT[CT]^[GA]AC
1849 GTTGAC
Ksp632I 167 CTCTTC CTCTTC(1/4)
1487 GAAGAG
MfeI 501 CAATTG C^AATTG
1250 CAATTG
MslI 1072 CATGTGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1381 CATGTTTATG
MunI 501 CAATTG C^AATTG
1250 CAATTG
NlaIV 1329 GGCACC GG[ACGT]^[ACGT]CC
1834 GGATCC
PstI 439 CTGCAG CTGCA^G
1767 CTGCAG
RsaI 901 GTAC GT^AC
1322 GTAC
Sau96I 1045 GGACC G^G[ACGT]CC
1237 GGCCC
SfcI 439 CTGCAG C^T[GA][CT]AG
1767 CTGCAG
SmlI 211 CTCAAG C^T[CT][GA]AG
885 CTTGAG
StyI 934 CCAAGG C^C[AT][AT]GG
1220 CCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 43 enzymes) #
#########################################
AciI AluI AlwI BbvI BfaI
BsaJI BsiYI BslI BsrDI BsrI
BstF5I BstXI BstYI Cac8I DdeI
DpnII Fnu4HI FokI HindIII HinfI
HphI ItaI MaeI MaeIII MboI
MboII MflI MnlI MseI MwoI
NdeII NlaIII Sau3AI SfaNI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII