Restriction map for
pF1KA1376
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# NO CUT ENZYMES ( 148 enzymes) #
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AatII AccI AccIII AclI AcyI
AflIII AgeI AhdI Alw44I AlwNI
Aor51HI ApaI ApaLI AscI AseI
AsnI AspEI AspHI AspI AvaI
AviII AvrII BalI BamHI BbeI
BbrPI BbsI BbvCI BcgI BcnI
BglII BlnI BlpI Bpu1102I BpuAI
BsaAI BsaHI BsaI BsaWI BseAI
BseRI BsgI BsiEI BsiHKAI BsiWI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrGI BssHII
BssSI Bst1107I BstAPI BstEII BstPI
BstXI BstYI BstZ17I CelII ClaI
CpoI DraI DraII DraIII DsaI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO109I EcoO65I EcoRI
FauI FseI FspI HaeII HgaI
Hin1I HincII HindII HpaI KasI
KspI MfeI MflI MluI MluNI
MroI MscI MspA1I MunI NarI
NciI NcoI NdeI NheI NotI
NruI PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SacI
SacII SalI SbfI ScaI SexAI
SfaNI SfiI SfoI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SwaI Tth111I Van91I XcmI XhoI
XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 57 enzymes) #
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Acc65I 928 GGTACC G^GTACC
AccII 850 CGCG CG^CG
AflII 576 CTTAAG C^TTAAG
AlwI 1192 GATCC GGATC(4/5)
AocI 560 CCTCAGG CC^T[ACGT]AGG
Asp700I 616 GAATCAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 928 GGTACC G^GTACC
AvaII 1129 GGACC G^G[AT]CC
BanII 1118 GAGCCC G[GA]GC[CT]^C
BbvI 676 GCAGC GCAGC(8/12)
BciVI 81 GGATAC GTATCC(6/5)
BclI 1203 TGATCA T^GATCA
BfrI 576 CTTAAG C^TTAAG
BglI 386 GCCGACATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 887 CTGGAG CTGGAG(16/14)
BsaBI 1192 GATCCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmFI 61 GTCCC GGGAC(10/14)
BsmI 337 GCATTC GAATGC(1/-1)
BsrDI 56 GCAATG GCAATG(2/0)
BsrFI 193 GCCGGC [GA]^CCGG[CT]
BstBI 379 TTCGAA TT^CGAA
BstUI 850 CGCG CG^CG
Bsu36I 560 CCTCAGG CC^T[ACGT]AGG
CfoI 402 GCGC GCG^C
Cfr10I 193 GCCGGC [GA]^CCGG[CT]
DrdI 52 GACAGCAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 560 CCTCAGG CC^T[ACGT]AGG
EcoRV 487 GATATC GAT^ATC
EcoT14I 433 CCTTGG C^C[AT][AT]GG
EcoT22I 335 ATGCAT ATGCA^T
FbaI 1203 TGATCA T^GATCA
Fnu4HI 676 GCAGC GC^[ACGT]GC
HapII 194 CCGG C^CGG
HhaI 402 GCGC GCG^C
HinP1I 402 GCGC G^CGC
HindIII 33 AAGCTT A^AGCTT
HpaII 194 CCGG C^CGG
ItaI 676 GCAGC GC^[ACGT]GC
KpnI 928 GGTACC GGTAC^C
MaeII 784 ACGT A^CGT
MamI 1192 GATCCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspI 194 CCGG C^CGG
MvnI 850 CGCG CG^CG
NaeI 193 GCCGGC GCC^GGC
NgoMIV 193 GCCGGC G^CCGGC
NsiI 335 ATGCAT ATGCA^T
NspI 159 ACATGC [GA]CATG^[CT]
NspV 379 TTCGAA TT^CGAA
SapI 650 GCTCTTC GCTCTTC(1/4)
SfcI 837 CTGTAG C^T[GA][CT]AG
SfuI 379 TTCGAA TT^CGAA
SspI 857 AATATT AAT^ATT
StyI 433 CCTTGG C^C[AT][AT]GG
TseI 676 GCAGC G^C[AT]GC
Tsp45I 510 GTCAC ^GT[GC]AC
XbaI 184 TCTAGA T^CTAGA
XmnI 616 GAATCAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AciI 849 CCGC CCGC(-3/-1)
1074 GCGG
BanI 666 GGTGCC G^G[CT][GA]CC
928 GGTACC
BfaI 74 CTAG C^TAG
185 CTAG
BmyI 268 GGGCAC G[AGT]GC[ACT]^C
1118 GAGCCC
BsaJI 433 CCTTGG C^C[ACGT][ACGT]GG
609 CCCAGG
BsmAI 781 GAGAC GTCTC(1/5)
88 GTCTC
BsmBI 87 CGTCTC CGTCTC(1/5)
781 GAGACG
Bsp1286I 268 GGGCAC G[AGT]GC[ACT]^C
1118 GAGCCC
BstF5I 943 CATCC GGATG(2/0)
1226 CATCC
Cac8I 193 GCCGGC GC[ACGT]^[ACGT]GC
520 GCAGGC
Cfr13I 565 GGCCC G^G[ACGT]CC
1129 GGACC
DpnII 1192 GATC ^GATC
1204 GATC
EarI 651 CTCTTC CTCTTC(1/4)
18 GAAGAG
FokI 943 CATCC GGATG(9/13)
1226 CATCC
Ksp632I 651 CTCTTC CTCTTC(1/4)
18 GAAGAG
MaeI 74 CTAG C^TAG
185 CTAG
MboI 1192 GATC ^GATC
1204 GATC
NdeII 1192 GATC ^GATC
1204 GATC
Sau3AI 1192 GATC ^GATC
1204 GATC
Sau96I 565 GGCCC G^G[ACGT]CC
1129 GGACC
SmlI 576 CTTAAG C^T[CT][GA]AG
963 CTCAAG
StuI 430 AGGCCT AGG^CCT
695 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 31 enzymes) #
#########################################
AcsI AfaI AluI ApoI BsiYI
BslI BsrI BssKI BstNI DdeI
EcoRII HaeIII HinfI HphI MaeIII
MboII MnlI MseI MslI MvaI
MwoI NlaIII NlaIV RsaI ScrFI
TaqI TfiI Tru9I Tsp509I TspRI
TthHB8I