Restriction map for
pF1KA1373
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# NO CUT ENZYMES ( 149 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AflII AflIII AgeI AhdI
Alw44I ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AspHI AspI
AviII AvrII BalI BanI BbeI
BbrPI BbvCI BcgI BciVI BclI
BcnI BfrI BglI BlnI BlpI
Bpu1102I BsaAI BsaBI BsaI BsaWI
BseAI BsiEI BsiHKAI BsiWI BsmAI
BsmBI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstEII
BstPI BstUI BstXI BstZ17I CelII
Cfr10I ClaI CpoI DraIII DrdI
DsaI EaeI EagI Eam1105I EclXI
Eco52I EcoNI EcoO65I EcoRV EcoT22I
FbaI FseI FspI HapII HincII
HindII HpaI HpaII KasI KpnI
KspI MamI MfeI MluI MluNI
MroI MscI MspI MunI MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NotI NruI NsiI PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacI SacII SalI SbfI ScaI
SexAI SfcI SfiI SfoI SgrAI
SmaI SnaBI SpeI Sse8387I SspBI
StuI SwaI Tth111I Van91I XbaI
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AcyI 183 GGCGTC G[GA]^CG[CT]C
AfaI 298 GTAC GT^AC
AocI 596 CCTCAGG CC^T[ACGT]AGG
Aor51HI 625 AGCGCT AGC^GCT
Asp700I 259 GAAAAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 569 CCCGAG C^[CT]CG[GA]G
BamHI 1034 GGATCC G^GATCC
BbsI 130 GAAGAC GAAGAC(2/6)
BglII 819 AGATCT A^GATCT
BpmI 161 CTGGAG CTGGAG(16/14)
BpuAI 130 GAAGAC GAAGAC(2/6)
BsaHI 183 GGCGTC G[GA]^CG[CT]C
BsgI 293 GTGCAG GTGCAG(16/14)
BsmFI 954 GGGAC GGGAC(10/14)
BsmI 340 GCATTC GAATGC(1/-1)
BsoBI 569 CCCGAG C^[CT]CG[GA]G
BssKI 1241 CCAGG ^CC[ACGT]GG
BstBI 538 TTCGAA TT^CGAA
BstNI 1241 CCAGG CC^[AT]GG
Bsu36I 596 CCTCAGG CC^T[ACGT]AGG
DraI 375 TTTAAA TTT^AAA
DraII 739 AGGGCCT [GA]G^G[ACGT]CC[CT]
EarI 79 GAAGAG CTCTTC(1/4)
Eco47III 625 AGCGCT AGC^GCT
Eco81I 596 CCTCAGG CC^T[ACGT]AGG
EcoO109I 739 AGGGCCT [GA]G^G[ACGT]CC[CT]
EcoRI 21 GAATTC G^AATTC
EcoRII 1241 CCAGG ^CC[AT]GG
FauI 952 GCGGG CCCGC(4/6)
HaeII 625 AGCGCT [GA]GCGC^[CT]
HgaI 184 GCGTC GACGC(5/10)
Hin1I 183 GGCGTC G[GA]^CG[CT]C
Ksp632I 79 GAAGAG CTCTTC(1/4)
MslI 55 CATACAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 752 CAGCTG C[AC]G^C[GT]G
MvaI 1241 CCAGG CC^[AT]GG
NheI 715 GCTAGC G^CTAGC
NspI 1184 GCATGC [GA]CATG^[CT]
NspV 538 TTCGAA TT^CGAA
PleI 86 GAGTC GAGTC(4/5)
PvuII 752 CAGCTG CAG^CTG
RsaI 298 GTAC GT^AC
SapI 79 GAAGAGC GCTCTTC(1/4)
ScrFI 1241 CCAGG CC^[ACGT]GG
SfuI 538 TTCGAA TT^CGAA
SphI 1184 GCATGC GCATG^C
SspI 404 AATATT AAT^ATT
Tsp45I 968 GTGAC ^GT[GC]AC
XmnI 259 GAAAAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AciI 486 GCGG CCGC(-3/-1)
952 GCGG
AlwNI 296 CAGTACCTG CAG[ACGT][ACGT][ACGT]^CTG
526 CAGTTTCTG
AvaII 955 GGACC G^G[AT]CC
1324 GGACC
BanII 1237 GAGCCC G[GA]GC[CT]^C
1303 GAGCCC
BseRI 725 CTCCTC GAGGAG(10/8)
1018 CTCCTC
BsiYI 1338 CCCACTCTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1339 CCACTCTTGGG
BslI 1338 CCCACTCTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1339 CCACTCTTGGG
BsrDI 1122 CATTGC GCAATG(2/0)
288 GCAATG
BsrI 843 ACTGG ACTGG(1/-1)
1156 ACTGG
CfoI 501 GCGC GCG^C
626 GCGC
EcoT14I 346 CCAAGG C^C[AT][AT]GG
1232 CCAAGG
HaeIII 741 GGCC GG^CC
1119 GGCC
HhaI 501 GCGC GCG^C
626 GCGC
HinP1I 501 GCGC G^CGC
626 GCGC
HindIII 103 AAGCTT A^AGCTT
262 AAGCTT
MaeII 147 ACGT A^CGT
389 ACGT
MaeIII 149 GTTAC ^GT[ACGT]AC
968 GTGAC
MseI 376 TTAA T^TAA
745 TTAA
SmlI 550 CTCAAG C^T[CT][GA]AG
1189 CTTGAG
StyI 346 CCAAGG C^C[AT][AT]GG
1232 CCAAGG
TaqI 276 TCGA T^CGA
539 TCGA
Tru9I 376 TTAA T^TAA
745 TTAA
TthHB8I 276 TCGA T^CGA
539 TCGA
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# 3 OR MORE CUT ENZYMES ( 37 enzymes) #
#########################################
AcsI AluI AlwI ApoI BbvI
BfaI BmyI BsaJI Bsp1286I BstF5I
BstYI Cac8I Cfr13I DdeI DpnII
Fnu4HI FokI HinfI HphI ItaI
MaeI MboI MboII MflI MnlI
MwoI NdeII NlaIII NlaIV Sau3AI
Sau96I SfaNI TfiI TseI Tsp509I
TspRI XhoII