Restriction map for
pF1KA1346
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# NO CUT ENZYMES ( 89 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AgeI AhdI Alw44I AocI Aor51HI
ApaLI AscI Asp718I AspEI AspI
AviII AvrII BalI BciVI BclI
BfrI BglI BlnI BlpI Bpu1102I
BsaBI BspDI BssSI Bst1107I BstBI
BstEII BstPI BstZ17I Bsu36I CelII
ClaI CpoI Eam1105I Eco47III Eco81I
EcoO65I EcoT22I FbaI FseI FspI
HincII HindII HpaI KpnI MamI
MfeI MluI MluNI MscI MunI
NcoI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmeI PshAI Psp1406I PstI
PvuI RsrII SacI SalI SapI
SbfI SexAI SfiI SfuI SgrAI
SnaBI SphI Sse8387I SspI StuI
SwaI Tth111I XbaI XhoI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AccIII 345 TCCGGA T^CCGGA
ApaI 91 GGGCCC GGGCC^C
AseI 2650 ATTAAT AT^TAAT
AsnI 2650 ATTAAT AT^TAAT
AspHI 1042 GAGCAC G[AT]GC[AT]^C
BamHI 2278 GGATCC G^GATCC
BbvCI 414 CCTCAGC CCTCAGC(-5/-2)
BcgI 796 GCAGACCAGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 923 AGATCT A^GATCT
BsaWI 345 TCCGGA [AT]^CCGG[AT]
BseAI 345 TCCGGA T^CCGGA
BsiEI 616 CGGCCG CG[GA][CT]^CG
BsiHKAI 1042 GAGCAC G[AT]GC[AT]^C
BsiWI 1442 CGTACG C^GTACG
BspEI 345 TCCGGA T^CCGGA
BspMI 449 ACCTGC ACCTGC(4/8)
BsrBI 64 GAGCGG CCGCTC(-3/-3)
BsrDI 2486 GCAATG GCAATG(2/0)
BssHII 198 GCGCGC G^CGCGC
BstAPI 1145 GCAGAAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraI 2850 TTTAAA TTT^AAA
DrdI 1759 GACAACCCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 616 CGGCCG C^GGCCG
EarI 2581 GAAGAG CTCTTC(1/4)
EclXI 616 CGGCCG C^GGCCG
Eco52I 616 CGGCCG C^GGCCG
EcoNI 420 CCTCTGCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
Ksp632I 2581 GAAGAG CTCTTC(1/4)
KspI 119 CCGCGG CCGC^GG
MroI 345 TCCGGA T^CCGGA
NaeI 531 GCCGGC GCC^GGC
NgoMIV 531 GCCGGC G^CCGGC
PshBI 2650 ATTAAT AT^TAAT
SacII 119 CCGCGG CCGC^GG
ScaI 1787 AGTACT AGT^ACT
SmaI 204 CCCGGG CCC^GGG
SpeI 2194 ACTAGT A^CTAGT
XcmI 1464 CCAGTTTACATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 204 CCCGGG C^CCGGG
XmaI 204 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 29 enzymes) #
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AcsI 814 GAATTC [GA]^AATT[CT]
2429 GAATTC
ApoI 814 GAATTC [GA]^AATT[CT]
2429 GAATTC
Asp700I 1930 GAATGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2425 GAAAGAATTC
BbrPI 588 CACGTG CAC^GTG
1213 CACGTG
BbsI 640 GAAGAC GAAGAC(2/6)
2627 GAAGAC
BfaI 178 CTAG C^TAG
2195 CTAG
BpuAI 640 GAAGAC GAAGAC(2/6)
2627 GAAGAC
BsaAI 588 CACGTG [CT]AC^GT[GA]
1213 CACGTG
BsgI 232 CTGCAC GTGCAG(16/14)
2711 GTGCAG
BsmBI 1953 CGTCTC CGTCTC(1/5)
334 GAGACG
Bsp1407I 875 TGTACA T^GTACA
2378 TGTACA
BspHI 2214 TCATGA T^CATGA
2832 TCATGA
BspLU11I 1096 ACATGT A^CATGT
1513 ACATGT
BsrGI 875 TGTACA T^GTACA
2378 TGTACA
BstXI 785 CCATGCTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
819 CCACGGCAGTGG
DraIII 1022 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
1521 CACCTTGTG
EaeI 131 TGGCCG [CT]^GGCC[GA]
616 CGGCCG
EcoRI 814 GAATTC G^AATTC
2429 GAATTC
EcoRV 2210 GATATC GAT^ATC
2218 GATATC
HindIII 1901 AAGCTT A^AGCTT
2793 AAGCTT
MaeI 178 CTAG C^TAG
2195 CTAG
PmaCI 588 CACGTG CAC^GTG
1213 CACGTG
PmlI 588 CACGTG CAC^GTG
1213 CACGTG
PpuMI 695 AGGACCC [GA]G^G[AT]CC[CT]
1087 GGGTCCC
RcaI 2214 TCATGA T^CATGA
2832 TCATGA
SfcI 541 CTACAG C^T[GA][CT]AG
1812 CTACAG
SmlI 1828 CTTGAG C^T[CT][GA]AG
2394 CTTGAG
SspBI 875 TGTACA T^GTACA
2378 TGTACA
XmnI 1930 GAATGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2425 GAAAGAATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI AlwNI AvaI AvaII
BanI BanII BbeI BbvI BcnI
BmyI BpmI BsaHI BsaI BsaJI
BseRI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BsrFI BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NarI
NciI NdeII NlaIII NlaIV NspI
PflMI PleI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfoI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoII