Restriction map for
pF1KA1331
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# NO CUT ENZYMES ( 115 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AcsI AflII AflIII AhdI Alw44I
Aor51HI ApaLI ApoI AscI Asp718I
AspEI AspHI AspI AviII BalI
BbeI BbrPI BbvCI BcgI BclI
BfrI BglI BglII BlpI Bpu1102I
BsaAI BsaI BsgI BsiHKAI BsiWI
BsmBI BsmI BspDI BspHI BspLU11I
BspMI BsrBI BssHII Bst1107I BstUI
BstXI BstZ17I CelII ClaI CpoI
DraI DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoRI EcoRV
EcoT22I FauI FbaI FseI FspI
HincII HindII HindIII HpaI KasI
KpnI KspI MaeII MfeI MluI
MluNI MscI MslI MunI MvnI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI PacI PflFI
PmaCI PmeI PmlI PshAI Psp1406I
PstI PvuI RcaI RsrII SacI
SacII SalI SbfI SfiI SfoI
SgrAI SnaBI SpeI SphI Sse8387I
SspI StuI SwaI Tth111I XbaI
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# ONE CUT ENZYMES ( 66 enzymes) #
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AccIII 1724 TCCGGA T^CCGGA
AcyI 593 GGCGTC G[GA]^CG[CT]C
AgeI 50 ACCGGT A^CCGGT
AocI 538 CCTCAGG CC^T[ACGT]AGG
ApaI 71 GGGCCC GGGCC^C
AseI 1246 ATTAAT AT^TAAT
AsnI 1246 ATTAAT AT^TAAT
AvrII 1137 CCTAGG C^CTAGG
BamHI 331 GGATCC G^GATCC
BanII 71 GGGCCC G[GA]GC[CT]^C
BbsI 479 GAAGAC GAAGAC(2/6)
BbvI 196 GCTGC GCAGC(8/12)
BciVI 940 GGATAC GTATCC(6/5)
BlnI 1137 CCTAGG C^CTAGG
BpmI 949 CTGGAG CTGGAG(16/14)
BpuAI 479 GAAGAC GAAGAC(2/6)
BsaBI 1567 GATCTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 593 GGCGTC G[GA]^CG[CT]C
BseAI 1724 TCCGGA T^CCGGA
Bsp1407I 2 TGTACA T^GTACA
BspEI 1724 TCCGGA T^CCGGA
BsrDI 1004 GCAATG GCAATG(2/0)
BsrFI 50 ACCGGT [GA]^CCGG[CT]
BsrGI 2 TGTACA T^GTACA
BssSI 1609 CACGAG CACGAG(-5/-1)
BstAPI 1707 GCACCAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 297 TTCGAA TT^CGAA
BstEII 1348 GGTTACC G^GT[ACGT]ACC
BstPI 1348 GGTTACC G^GT[ACGT]ACC
Bsu36I 538 CCTCAGG CC^T[ACGT]AGG
Cac8I 199 GCCAGC GC[ACGT]^[ACGT]GC
CfoI 90 GCGC GCG^C
Cfr10I 50 ACCGGT [GA]^CCGG[CT]
DraIII 169 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 489 TGGCCG [CT]^GGCC[GA]
Eco81I 538 CCTCAGG CC^T[ACGT]AGG
EcoO65I 1348 GGTTACC G^GT[ACGT]ACC
HaeII 89 GGCGCT [GA]GCGC^[CT]
HgaI 594 GCGTC GACGC(5/10)
HhaI 90 GCGC GCG^C
Hin1I 593 GGCGTC G[GA]^CG[CT]C
HinP1I 90 GCGC G^CGC
MamI 1567 GATCTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 1724 TCCGGA T^CCGGA
NspI 344 ACATGC [GA]CATG^[CT]
NspV 297 TTCGAA TT^CGAA
PaeR7I 1193 CTCGAG C^TCGAG
PflMI 755 CCAAAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 50 ACCGGT A^CCGGT
PpuMI 837 GGGACCT [GA]G^G[AT]CC[CT]
PshBI 1246 ATTAAT AT^TAAT
SapI 769 GAAGAGC GCTCTTC(1/4)
ScaI 1315 AGTACT AGT^ACT
SexAI 483 ACCAGGT A^CC[AT]GGT
SfaNI 1713 GATGC GCATC(5/9)
SfcI 113 CTACAG C^T[GA][CT]AG
SfuI 297 TTCGAA TT^CGAA
SmaI 68 CCCGGG CCC^GGG
SmlI 1193 CTCGAG C^T[CT][GA]AG
SspBI 2 TGTACA T^GTACA
TseI 196 GCTGC G^C[AT]GC
Van91I 755 CCAAAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 230 CCACAAAGCCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1193 CTCGAG C^TCGAG
XmaCI 68 CCCGGG C^CCGGG
XmaI 68 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AciI 987 CCGC CCGC(-3/-1)
87 GCGG
Asp700I 1399 GAAATTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1744 GAAATCCTTC
BcnI 68 CCCGG CC^[GC]GG
69 CCGGG
BfaI 1138 CTAG C^TAG
1679 CTAG
BmyI 58 GTGCCC G[AGT]GC[ACT]^C
71 GGGCCC
BsaWI 50 ACCGGT [AT]^CCGG[AT]
1724 TCCGGA
BseRI 613 GAGGAG GAGGAG(10/8)
1196 GAGGAG
BsiEI 48 CGACCG CG[GA][CT]^CG
52 CGGTCG
BsmAI 1039 GAGAC GTCTC(1/5)
185 GTCTC
Bsp1286I 58 GTGCCC G[AGT]GC[ACT]^C
71 GGGCCC
BstF5I 1155 GGATG GGATG(2/0)
1539 GGATG
BstYI 331 GGATCC [GA]^GATC[CT]
1508 GGATCT
DraII 71 GGGCCCT [GA]G^G[ACGT]CC[CT]
837 GGGACCT
DsaI 239 CCATGG C^C[GA][CT]GG
1143 CCATGG
EcoO109I 71 GGGCCCT [GA]G^G[ACGT]CC[CT]
837 GGGACCT
Fnu4HI 87 GCGGC GC^[ACGT]GC
196 GCTGC
FokI 1155 GGATG GGATG(9/13)
1539 GGATG
HphI 1446 GGTGA GGTGA(8/7)
168 TCACC
ItaI 87 GCGGC GC^[ACGT]GC
196 GCTGC
MaeI 1138 CTAG C^TAG
1679 CTAG
MflI 331 GGATCC [GA]^GATC[CT]
1508 GGATCT
MspA1I 223 CAGCTG C[AC]G^C[GT]G
1624 CAGCTG
NciI 68 CCCGG CC^[GC]GG
69 CCGGG
NcoI 239 CCATGG C^CATGG
1143 CCATGG
PvuII 223 CAGCTG CAG^CTG
1624 CAGCTG
TaqI 298 TCGA T^CGA
1194 TCGA
Tsp45I 383 GTGAC ^GT[GC]AC
403 GTGAC
TthHB8I 298 TCGA T^CGA
1194 TCGA
XhoII 331 GGATCC [GA]^GATC[CT]
1508 GGATCT
XmnI 1399 GAAATTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1744 GAAATCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AfaI AluI AlwI AlwNI AvaI
AvaII BanI BsaJI BsiYI BslI
BsmFI BsoBI BsrI BssKI BstNI
Cfr13I DdeI DpnII EarI EcoRII
EcoT14I HaeIII HapII HinfI HpaII
Ksp632I MaeIII MboI MboII MnlI
MseI MspI MvaI MwoI NdeII
NlaIII NlaIV PleI RsaI Sau3AI
Sau96I ScrFI StyI TfiI Tru9I
Tsp509I TspRI