Restriction map for
pF1KA1310
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# NO CUT ENZYMES ( 109 enzymes) #
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Acc65I AccIII AclI AflII AgeI
Alw44I Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspI
AviII AvrII BamHI BbeI BbrPI
BcgI BclI BfaI BfrI BglII
BlnI BlpI Bpu1102I BsaAI BseAI
BsiWI BsmI Bsp1407I BspDI BspEI
BspHI BsrDI BsrGI BssHII Bst1107I
BstBI BstEII BstPI BstZ17I CelII
ClaI CpoI DraI DrdI EagI
EclXI Eco47III Eco52I EcoO65I EcoT22I
FbaI FseI FspI HaeII HindIII
HpaI KasI KpnI KspI MaeI
MfeI MluI MroI MseI MunI
NaeI NarI NgoMIV NheI NotI
NruI NsiI NspV PacI PflFI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RcaI RsrII SacII
SbfI ScaI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SspBI SspI SwaI Tru9I Tth111I
XbaI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AatII 223 GACGTC GACGT^C
AccI 1532 GTCGAC GT^[AC][GT]AC
AccII 1483 CGCG CG^CG
AcsI 1218 GAATTC [GA]^AATT[CT]
AcyI 223 GACGTC G[GA]^CG[CT]C
AfaI 209 GTAC GT^AC
AflIII 1695 ACATGT A^C[GA][CT]GT
AhdI 603 GACCTTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1571 CCTCAGG CC^T[ACGT]AGG
ApoI 1218 GAATTC [GA]^AATT[CT]
AspEI 603 GACCTTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbsI 1188 GAAGAC GAAGAC(2/6)
BciVI 561 GGATAC GTATCC(6/5)
BpuAI 1188 GAAGAC GAAGAC(2/6)
BsaHI 223 GACGTC G[GA]^CG[CT]C
BsaWI 680 ACCGGA [AT]^CCGG[AT]
BsiEI 1927 CGACCG CG[GA][CT]^CG
BsmBI 241 GAGACG CGTCTC(1/5)
BspLU11I 1695 ACATGT A^CATGT
BsrBI 1522 GAGCGG CCGCTC(-3/-3)
BsrFI 449 ACCGGC [GA]^CCGG[CT]
BssSI 1418 CTCGTG CACGAG(-5/-1)
BstUI 1483 CGCG CG^CG
Bsu36I 1571 CCTCAGG CC^T[ACGT]AGG
CfoI 498 GCGC GCG^C
Cfr10I 449 ACCGGC [GA]^CCGG[CT]
DraIII 2162 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1244 CCATGG C^C[GA][CT]GG
Eam1105I 603 GACCTTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1571 CCTCAGG CC^T[ACGT]AGG
EcoNI 1987 CCTTCCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1218 GAATTC G^AATTC
EcoRV 2278 GATATC GAT^ATC
HhaI 498 GCGC GCG^C
Hin1I 223 GACGTC G[GA]^CG[CT]C
HinP1I 498 GCGC G^CGC
MaeII 224 ACGT A^CGT
MvnI 1483 CGCG CG^CG
NcoI 1244 CCATGG C^CATGG
NdeI 979 CATATG CA^TATG
PflMI 125 CCAAGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 758 GGGACCC [GA]G^G[AT]CC[CT]
PshAI 1191 GACTCCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsaI 209 GTAC GT^AC
SacI 2068 GAGCTC GAGCT^C
SalI 1532 GTCGAC G^TCGAC
SexAI 98 ACCTGGT A^CC[AT]GGT
SphI 173 GCATGC GCATG^C
Tsp509I 1219 AATT ^AATT
Van91I 125 CCAAGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 355 CCACCAGAGGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 16 enzymes) #
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BalI 464 TGGCCA TGG^CCA
2198 TGGCCA
BanI 52 GGCACC G^G[CT][GA]CC
1798 GGCACC
BanII 1437 GGGCTC G[GA]GC[CT]^C
2068 GAGCTC
BglI 457 GCCCGCTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2329 GCCCCACAGGC
BsaBI 819 GATTCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1306 GATGACAATC
BsaI 601 GAGACC GGTCTC(1/5)
2145 GGTCTC
BspMI 1536 ACCTGC ACCTGC(4/8)
2014 GCAGGT
EaeI 464 TGGCCA [CT]^GGCC[GA]
2198 TGGCCA
HgaI 44 GACGC GACGC(5/10)
648 GACGC
MamI 819 GATTCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1306 GATGACAATC
MluNI 464 TGGCCA TGG^CCA
2198 TGGCCA
MscI 464 TGGCCA TGG^CCA
2198 TGGCCA
PaeR7I 973 CTCGAG C^TCGAG
1893 CTCGAG
SapI 63 GCTCTTC GCTCTTC(1/4)
1786 GCTCTTC
StuI 728 AGGCCT AGG^CCT
2256 AGGCCT
XhoI 973 CTCGAG C^TCGAG
1893 CTCGAG
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# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AciI AluI AlwI AlwNI AspHI
AvaI AvaII BbvCI BbvI BcnI
BmyI BpmI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrI BssKI BstAPI
BstF5I BstNI BstXI BstYI Cac8I
Cfr13I DdeI DpnII DraII EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeIII MboI MboII MflI MnlI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PstI PvuII Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI TseI Tsp45I TspRI
TthHB8I XhoII