Restriction map for
pF1KA1301
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# NO CUT ENZYMES ( 59 enzymes) #
##################################
AclI AgeI AhdI Aor51HI AscI
AseI AsnI AspEI AviII AvrII
BbeI BbvCI BcgI BglI BlnI
BsiWI BspDI BssHII Bst1107I BstZ17I
ClaI CpoI DrdI EagI Eam1105I
EclXI Eco47III Eco52I FseI FspI
HindIII HpaI KasI MfeI MluI
MunI NaeI NarI NgoMIV NheI
NotI PacI PaeR7I PinAI PmeI
PshAI PshBI Psp1406I PvuI RsrII
SalI SfiI SfoI SgrAI SnaBI
SpeI SphI SwaI XhoI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AatII 1232 GACGTC GACGT^C
AccIII 2870 TCCGGA T^CCGGA
AflII 625 CTTAAG C^TTAAG
Alw44I 2103 GTGCAC G^TGCAC
ApaI 382 GGGCCC GGGCC^C
ApaLI 2103 GTGCAC G^TGCAC
Asp700I 1591 GAAAATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 1791 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 1278 TGGCCA TGG^CCA
BamHI 3935 GGATCC G^GATCC
BfrI 625 CTTAAG C^TTAAG
BsaBI 405 GATAAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1789 GAGACC GGTCTC(1/5)
BseAI 2870 TCCGGA T^CCGGA
BsiEI 2509 CGACCG CG[GA][CT]^CG
BsmBI 690 GAGACG CGTCTC(1/5)
Bsp1407I 4254 TGTACA T^GTACA
BspEI 2870 TCCGGA T^CCGGA
BspHI 978 TCATGA T^CATGA
BsrBI 171 CCGCTC CCGCTC(-3/-3)
BsrDI 1934 GCAATG GCAATG(2/0)
BsrGI 4254 TGTACA T^GTACA
BssSI 1408 CACGAG CACGAG(-5/-1)
BstBI 3577 TTCGAA TT^CGAA
BstEII 1429 GGTTACC G^GT[ACGT]ACC
BstPI 1429 GGTTACC G^GT[ACGT]ACC
EcoO65I 1429 GGTTACC G^GT[ACGT]ACC
EcoT22I 1757 ATGCAT ATGCA^T
HaeII 2326 GGCGCT [GA]GCGC^[CT]
KspI 2962 CCGCGG CCGC^GG
MamI 405 GATAAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1278 TGGCCA TGG^CCA
MroI 2870 TCCGGA T^CCGGA
MscI 1278 TGGCCA TGG^CCA
NcoI 1319 CCATGG C^CATGG
NdeI 3443 CATATG CA^TATG
NruI 2939 TCGCGA TCG^CGA
NsiI 1757 ATGCAT ATGCA^T
NspV 3577 TTCGAA TT^CGAA
PflFI 1791 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 1213 AGGACCT [GA]G^G[AT]CC[CT]
RcaI 978 TCATGA T^CATGA
SacII 2962 CCGCGG CCGC^GG
SbfI 1624 CCTGCAGG CCTGCA^GG
ScaI 4699 AGTACT AGT^ACT
SexAI 152 ACCTGGT A^CC[AT]GGT
SfuI 3577 TTCGAA TT^CGAA
Sse8387I 1624 CCTGCAGG CCTGCA^GG
SspBI 4254 TGTACA T^GTACA
Tth111I 1791 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 3784 TCTAGA T^CTAGA
XmnI 1591 GAAAATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 31 enzymes) #
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AcyI 38 GGCGTC G[GA]^CG[CT]C
1232 GACGTC
AocI 1894 CCTGAGG CC^T[ACGT]AGG
2623 CCTGAGG
AvaI 14 CCCGGG C^[CT]CG[GA]G
3548 CCCGGG
BglII 570 AGATCT A^GATCT
2545 AGATCT
BlpI 894 GCTCAGC GC^T[ACGT]AGC
3414 GCTGAGC
Bpu1102I 894 GCTCAGC GC^T[ACGT]AGC
3414 GCTGAGC
BsaHI 38 GGCGTC G[GA]^CG[CT]C
1232 GACGTC
BsaWI 399 ACCGGA [AT]^CCGG[AT]
2870 TCCGGA
BsgI 2051 GTGCAG GTGCAG(16/14)
2684 GTGCAG
BsoBI 14 CCCGGG C^[CT]CG[GA]G
3548 CCCGGG
BspLU11I 2933 ACATGT A^CATGT
4615 ACATGT
BsrFI 2399 GCCGGT [GA]^CCGG[CT]
2579 ACCGGC
BstAPI 2239 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2532 GCAGGTGCTGC
BstXI 930 CCACGTGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2428 CCACCAAACTGG
Bsu36I 1894 CCTGAGG CC^T[ACGT]AGG
2623 CCTGAGG
CelII 894 GCTCAGC GC^T[ACGT]AGC
3414 GCTGAGC
Cfr10I 2399 GCCGGT [GA]^CCGG[CT]
2579 ACCGGC
DraI 418 TTTAAA TTT^AAA
952 TTTAAA
DraII 382 GGGCCCT [GA]G^G[ACGT]CC[CT]
1213 AGGACCT
DraIII 1971 CACACGGTG CAC[ACGT][ACGT][ACGT]^GTG
2496 CACGACGTG
Eco81I 1894 CCTGAGG CC^T[ACGT]AGG
2623 CCTGAGG
EcoNI 1222 CCTCCCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2077 CCTGCCGAAGG
EcoO109I 382 GGGCCCT [GA]G^G[ACGT]CC[CT]
1213 AGGACCT
EcoRI 297 GAATTC G^AATTC
4013 GAATTC
EcoRV 2585 GATATC GAT^ATC
4090 GATATC
HgaI 39 GCGTC GACGC(5/10)
3300 GCGTC
Hin1I 38 GGCGTC G[GA]^CG[CT]C
1232 GACGTC
SacI 102 GAGCTC GAGCT^C
1614 GAGCTC
SmaI 14 CCCGGG CCC^GGG
3548 CCCGGG
XmaCI 14 CCCGGG C^CCGGG
3548 CCCGGG
XmaI 14 CCCGGG C^CCGGG
3548 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
Acc65I AccI AccII AciI AcsI
AfaI AflIII AluI AlwI AlwNI
ApoI Asp718I AspHI AvaII BanI
BanII BbrPI BbsI BbvI BciVI
BclI BcnI BfaI BmyI BpmI
BpuAI BsaAI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DsaI EaeI
EarI EcoRII EcoT14I FauI FbaI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HincII HindII HinfI HpaII
HphI ItaI KpnI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PflMI PleI
PmaCI PmlI PstI PvuII RsaI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII