Restriction map for
pF1KA1273
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# NO CUT ENZYMES ( 92 enzymes) #
##################################
Acc65I AclI AflIII AgeI AhdI
Alw44I AlwNI AocI ApaLI AscI
AseI AsnI Asp718I AspEI AviII
AvrII BamHI BbrPI BcgI BciVI
BclI BglII BlnI BsaAI BsaBI
BsiWI BspDI BspLU11I BsrDI BssSI
Bst1107I BstBI BstEII BstPI BstXI
BstZ17I Bsu36I ClaI CpoI DraI
DraIII Eam1105I Eco81I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HindIII HpaI KpnI KspI MamI
MfeI MluI MunI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflMI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RsrII SacI
SacII SbfI ScaI SexAI SfiI
SfuI SgrAI SnaBI SphI Sse8387I
SspI StuI SwaI Van91I XbaI
XcmI XhoI
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# ONE CUT ENZYMES ( 57 enzymes) #
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AatII 723 GACGTC GACGT^C
AccIII 1618 TCCGGA T^CCGGA
AflII 1342 CTTAAG C^TTAAG
Aor51HI 278 AGCGCT AGC^GCT
ApaI 248 GGGCCC GGGCC^C
Asp700I 1161 GAACGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 1498 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 440 CCCGGG C^[CT]CG[GA]G
BanII 248 GGGCCC G[GA]GC[CT]^C
BbeI 1367 GGCGCC GGCGC^C
BbvCI 196 GCTGAGG CCTCAGC(-5/-2)
BfrI 1342 CTTAAG C^TTAAG
BlpI 1453 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1453 GCTCAGC GC^T[ACGT]AGC
BsaWI 1618 TCCGGA [AT]^CCGG[AT]
BseAI 1618 TCCGGA T^CCGGA
BsmBI 721 GAGACG CGTCTC(1/5)
BsoBI 440 CCCGGG C^[CT]CG[GA]G
Bsp1407I 893 TGTACA T^GTACA
BspEI 1618 TCCGGA T^CCGGA
BspHI 986 TCATGA T^CATGA
BsrGI 893 TGTACA T^GTACA
BstAPI 900 GCACATCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 1453 GCTCAGC GC^T[ACGT]AGC
DrdI 1264 GACGTGATGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1774 CGGCCG C^GGCCG
EarI 1084 CTCTTC CTCTTC(1/4)
EclXI 1774 CGGCCG C^GGCCG
Eco47III 278 AGCGCT AGC^GCT
Eco52I 1774 CGGCCG C^GGCCG
HincII 788 GTCGAC GT[CT]^[GA]AC
HindII 788 GTCGAC GT[CT]^[GA]AC
KasI 1367 GGCGCC G^GCGCC
Ksp632I 1084 CTCTTC CTCTTC(1/4)
MroI 1618 TCCGGA T^CCGGA
MseI 1343 TTAA T^TAA
NarI 1367 GGCGCC GG^CGCC
NcoI 1010 CCATGG C^CATGG
NdeI 1484 CATATG CA^TATG
NspI 1433 GCATGT [GA]CATG^[CT]
PflFI 1498 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 663 GACAGCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 139 CTGCAG CTGCA^G
RcaI 986 TCATGA T^CATGA
SalI 788 GTCGAC G^TCGAC
SapI 1083 GCTCTTC GCTCTTC(1/4)
SfcI 139 CTGCAG C^T[GA][CT]AG
SfoI 1367 GGCGCC GGC^GCC
SmaI 440 CCCGGG CCC^GGG
SpeI 33 ACTAGT A^CTAGT
SspBI 893 TGTACA T^GTACA
TfiI 569 GATTC G^A[AT]TC
Tru9I 1343 TTAA T^TAA
Tth111I 1498 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 440 CCCGGG C^CCGGG
XmaI 440 CCCGGG C^CCGGG
XmnI 1161 GAACGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccI 643 GTCTAC GT^[AC][GT]AC
788 GTCGAC
AcsI 324 GAATTT [GA]^AATT[CT]
1198 AAATTC
AlwI 183 GATCC GGATC(4/5)
492 GATCC
ApoI 324 GAATTT [GA]^AATT[CT]
1198 AAATTC
BalI 1214 TGGCCA TGG^CCA
1655 TGGCCA
BbsI 210 GAAGAC GAAGAC(2/6)
933 GAAGAC
BfaI 34 CTAG C^TAG
712 CTAG
BpmI 742 CTGGAG CTGGAG(16/14)
805 CTGGAG
BpuAI 210 GAAGAC GAAGAC(2/6)
933 GAAGAC
BsiEI 1770 CGGTCG CG[GA][CT]^CG
1774 CGGCCG
BsmI 468 GAATGC GAATGC(1/-1)
657 GAATGC
BspMI 20 ACCTGC ACCTGC(4/8)
1283 ACCTGC
BssHII 445 GCGCGC G^CGCGC
447 GCGCGC
BstYI 182 AGATCC [GA]^GATC[CT]
491 AGATCC
EcoNI 216 CCTGAGCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1506 CCTCACTGAGG
MaeI 34 CTAG C^TAG
712 CTAG
MflI 182 AGATCC [GA]^GATC[CT]
491 AGATCC
MluNI 1214 TGGCCA TGG^CCA
1655 TGGCCA
MscI 1214 TGGCCA TGG^CCA
1655 TGGCCA
PpuMI 1142 AGGACCT [GA]G^G[AT]CC[CT]
1502 GGGTCCT
SmlI 169 CTCAAG C^T[CT][GA]AG
1342 CTTAAG
Tsp509I 325 AATT ^AATT
1199 AATT
XhoII 182 AGATCC [GA]^GATC[CT]
491 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 86 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AspHI AvaII BanI BbvI BcnI
BglI BmyI BsaHI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrBI BsrFI
BsrI BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI MaeII MaeIII MboI MboII
MnlI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NciI NdeII
NgoMIV NlaIII NlaIV PleI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
StyI TaqI TseI Tsp45I TspRI
TthHB8I