Restriction map for
pF1KA1265
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# NO CUT ENZYMES ( 120 enzymes) #
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AatII Acc65I AccII AccIII AclI
AflII AgeI AhdI Alw44I AocI
ApaI ApaLI AscI Asp718I AspEI
AspHI AspI AvaI AviII AvrII
BbeI BbrPI BcgI BcnI BfrI
BlnI BlpI BpmI Bpu1102I BsaAI
BsaWI BseAI BsiEI BsiHKAI BsiWI
BsmFI BsoBI BspDI BspEI BspMI
BsrBI BsrFI BssHII BssSI BstBI
BstEII BstPI BstUI Bsu36I CelII
Cfr10I ClaI CpoI DraII DraIII
DrdI EagI Eam1105I EclXI Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV FauI FseI FspI HincII
HindII HpaI KasI KpnI KspI
MluI MroI MvnI NaeI NarI
NciI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI Psp1406I PvuI RsrII SacI
SacII SalI SapI SbfI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SspI StuI Tth111I
Van91I XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AccI 2197 GTATAC GT^[AC][GT]AC
AciI 528 CCGC CCGC(-3/-1)
AcyI 1360 GACGCC G[GA]^CG[CT]C
AflIII 942 ACATGT A^C[GA][CT]GT
Aor51HI 102 AGCGCT AGC^GCT
AseI 295 ATTAAT AT^TAAT
AsnI 295 ATTAAT AT^TAAT
AvaII 795 GGTCC G^G[AT]CC
BalI 312 TGGCCA TGG^CCA
BamHI 1972 GGATCC G^GATCC
BanII 2053 GGGCTC G[GA]GC[CT]^C
BbsI 2116 GTCTTC GAAGAC(2/6)
BbvCI 1905 GCTGAGG CCTCAGC(-5/-2)
BciVI 433 GTATCC GTATCC(6/5)
BglI 2215 GCCATGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI 2053 GGGCTC G[AGT]GC[ACT]^C
BpuAI 2116 GTCTTC GAAGAC(2/6)
BsaHI 1360 GACGCC G[GA]^CG[CT]C
BsaI 646 GAGACC GGTCTC(1/5)
BsmBI 1358 GAGACG CGTCTC(1/5)
Bsp1286I 2053 GGGCTC G[AGT]GC[ACT]^C
BspLU11I 942 ACATGT A^CATGT
Bst1107I 2197 GTATAC GTA^TAC
BstXI 2438 CCATTATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 2197 GTATAC GTA^TAC
CfoI 103 GCGC GCG^C
DraI 402 TTTAAA TTT^AAA
EaeI 312 TGGCCA [CT]^GGCC[GA]
EarI 1798 GAAGAG CTCTTC(1/4)
Eco47III 102 AGCGCT AGC^GCT
EcoT22I 511 ATGCAT ATGCA^T
HaeII 102 AGCGCT [GA]GCGC^[CT]
HapII 1694 CCGG C^CGG
HgaI 1360 GACGC GACGC(5/10)
HhaI 103 GCGC GCG^C
Hin1I 1360 GACGCC G[GA]^CG[CT]C
HinP1I 103 GCGC G^CGC
HindIII 1630 AAGCTT A^AGCTT
HpaII 1694 CCGG C^CGG
Ksp632I 1798 GAAGAG CTCTTC(1/4)
MluNI 312 TGGCCA TGG^CCA
MscI 312 TGGCCA TGG^CCA
MspA1I 2249 CAGCTG C[AC]G^C[GT]G
MspI 1694 CCGG C^CGG
NsiI 511 ATGCAT ATGCA^T
PleI 1705 GACTC GAGTC(4/5)
PshBI 295 ATTAAT AT^TAAT
PstI 2300 CTGCAG CTGCA^G
PvuII 2249 CAGCTG CAG^CTG
ScaI 2101 AGTACT AGT^ACT
SmlI 1681 CTCAAG C^T[CT][GA]AG
SphI 2234 GCATGC GCATG^C
SwaI 401 ATTTAAAT ATTT^AAAT
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# TWO CUT ENZYMES ( 16 enzymes) #
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AlwNI 1661 CAGATTCTG CAG[ACGT][ACGT][ACGT]^CTG
2294 CAGTCACTG
Asp700I 100 GAAGCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1628 GAAAGCTTTC
BanI 1048 GGTGCC G^G[CT][GA]CC
1928 GGCACC
BglII 885 AGATCT A^GATCT
978 AGATCT
BseRI 2206 CTCCTC GAGGAG(10/8)
1801 GAGGAG
BsgI 1871 CTGCAC GTGCAG(16/14)
2066 GTGCAG
BsmI 387 GCATTC GAATGC(1/-1)
1938 GCATTC
Bsp1407I 813 TGTACA T^GTACA
837 TGTACA
BsrGI 813 TGTACA T^GTACA
837 TGTACA
MfeI 2060 CAATTG C^AATTG
2192 CAATTG
MunI 2060 CAATTG C^AATTG
2192 CAATTG
NcoI 90 CCATGG C^CATGG
1953 CCATGG
NdeI 10 CATATG CA^TATG
508 CATATG
SexAI 744 ACCAGGT A^CC[AT]GGT
1178 ACCTGGT
SspBI 813 TGTACA T^GTACA
837 TGTACA
XmnI 100 GAAGCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1628 GAAAGCTTTC
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# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
BbvI BclI BfaI BsaBI BsaJI
BsiYI BslI BsmAI BspHI BsrDI
BsrI BssKI BstAPI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
DsaI EcoRII EcoT14I FbaI Fnu4HI
FokI HaeIII HinfI HphI ItaI
MaeI MaeII MaeIII MamI MboI
MboII MflI MnlI MseI MslI
MvaI MwoI NdeII NlaIII NlaIV
NspI RcaI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII