Restriction map for
pF1KA1256
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# NO CUT ENZYMES ( 70 enzymes) #
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AatII AccIII AclI AflII AgeI
AhdI Alw44I AocI Aor51HI ApaLI
AscI AspEI AviII AvrII BcgI
BfrI BglI BlnI BsaWI BseAI
BsiEI BsiWI BsmBI Bsp1407I BspDI
BspEI BsrGI BssHII BssSI Bsu36I
ClaI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoT22I
FseI FspI MluI MroI NcoI
NotI NruI NsiI PacI PflMI
PinAI PmeI PshAI Psp1406I PvuI
SalI SbfI ScaI SfiI SgrAI
SmaI SnaBI SpeI Sse8387I SspBI
SwaI Van91I XbaI XmaCI XmaI
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# ONE CUT ENZYMES ( 67 enzymes) #
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Acc65I 1662 GGTACC G^GTACC
AflIII 41 ACATGT A^C[GA][CT]GT
ApaI 4210 GGGCCC GGGCC^C
Asp718I 1662 GGTACC G^GTACC
AspI 1367 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 4063 CTCGAG C^[CT]CG[GA]G
BalI 932 TGGCCA TGG^CCA
BamHI 4139 GGATCC G^GATCC
BbeI 4625 GGCGCC GGCGC^C
BbrPI 4150 CACGTG CAC^GTG
BciVI 2148 GGATAC GTATCC(6/5)
BclI 126 TGATCA T^GATCA
BglII 1511 AGATCT A^GATCT
BlpI 3796 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 3796 GCTGAGC GC^T[ACGT]AGC
BsaBI 2576 GATCTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI 3938 GAATGC GAATGC(1/-1)
BsoBI 4063 CTCGAG C^[CT]CG[GA]G
BspHI 3561 TCATGA T^CATGA
BspLU11I 41 ACATGT A^CATGT
Bst1107I 611 GTATAC GTA^TAC
BstAPI 3106 GCATATGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 4892 TTCGAA TT^CGAA
BstEII 2946 GGTGACC G^GT[ACGT]ACC
BstPI 2946 GGTGACC G^GT[ACGT]ACC
BstZ17I 611 GTATAC GTA^TAC
CelII 3796 GCTGAGC GC^T[ACGT]AGC
CpoI 4124 CGGACCG CG^G[AT]CCG
DraI 3903 TTTAAA TTT^AAA
DsaI 3925 CCGCGG C^C[GA][CT]GG
EcoO65I 2946 GGTGACC G^GT[ACGT]ACC
EcoRI 1391 GAATTC G^AATTC
EcoRV 782 GATATC GAT^ATC
EcoT14I 3365 CCAAGG C^C[AT][AT]GG
FbaI 126 TGATCA T^GATCA
HaeII 4625 GGCGCC [GA]GCGC^[CT]
HpaI 3682 GTTAAC GTT^AAC
KasI 4625 GGCGCC G^GCGCC
KpnI 1662 GGTACC GGTAC^C
KspI 3925 CCGCGG CCGC^GG
MamI 2576 GATCTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 3533 CAATTG C^AATTG
MluNI 932 TGGCCA TGG^CCA
MscI 932 TGGCCA TGG^CCA
MunI 3533 CAATTG C^AATTG
NaeI 162 GCCGGC GCC^GGC
NarI 4625 GGCGCC GG^CGCC
NdeI 3107 CATATG CA^TATG
NgoMIV 162 GCCGGC G^CCGGC
NheI 3052 GCTAGC G^CTAGC
NspV 4892 TTCGAA TT^CGAA
PaeR7I 4063 CTCGAG C^TCGAG
PflFI 1367 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 4150 CACGTG CAC^GTG
PmlI 4150 CACGTG CAC^GTG
RcaI 3561 TCATGA T^CATGA
RsrII 4124 CGGACCG CG^G[AT]CCG
SacII 3925 CCGCGG CCGC^GG
SapI 3580 GAAGAGC GCTCTTC(1/4)
SexAI 2298 ACCTGGT A^CC[AT]GGT
SfoI 4625 GGCGCC GGC^GCC
SfuI 4892 TTCGAA TT^CGAA
SphI 362 GCATGC GCATG^C
StyI 3365 CCAAGG C^C[AT][AT]GG
Tth111I 1367 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 3484 CCAAGTCAACAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 4063 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 22 enzymes) #
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AccI 611 GTATAC GT^[AC][GT]AC
4480 GTCTAC
AccII 3926 CGCG CG^CG
4170 CGCG
Asp700I 2095 GAAAAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4344 GAAACTTTTC
BanI 1662 GGTACC G^G[CT][GA]CC
4625 GGCGCC
BsaAI 134 CACGTA [CT]AC^GT[GA]
4150 CACGTG
BsaI 4835 GAGACC GGTCTC(1/5)
3439 GGTCTC
BspMI 3288 ACCTGC ACCTGC(4/8)
4979 ACCTGC
BsrFI 162 GCCGGC [GA]^CCGG[CT]
3765 GCCGGT
BstUI 3926 CGCG CG^CG
4170 CGCG
Cfr10I 162 GCCGGC [GA]^CCGG[CT]
3765 GCCGGT
EaeI 932 TGGCCA [CT]^GGCC[GA]
3791 TGGCCG
HgaI 1142 GACGC GACGC(5/10)
4543 GCGTC
HincII 3488 GTCAAC GT[CT]^[GA]AC
3682 GTTAAC
HindII 3488 GTCAAC GT[CT]^[GA]AC
3682 GTTAAC
MslI 18 CACAGCTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
240 CATAGCTATG
MvnI 3926 CGCG CG^CG
4170 CGCG
PpuMI 700 GGGACCT [GA]G^G[AT]CC[CT]
4946 AGGTCCC
PstI 3831 CTGCAG CTGCA^G
4981 CTGCAG
SacI 3352 GAGCTC GAGCT^C
3694 GAGCTC
SspI 2940 AATATT AAT^ATT
3156 AATATT
StuI 2405 AGGCCT AGG^CCT
4164 AGGCCT
XmnI 2095 GAAAAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4344 GAAACTTTTC
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# 3 OR MORE CUT ENZYMES ( 99 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI AlwNI ApoI AseI AsnI
AspHI AvaII BanII BbsI BbvCI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaHI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrBI BsrDI BsrI BssKI
BstF5I BstNI BstXI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
EarI EcoNI EcoO109I EcoRII FauI
Fnu4HI FokI HaeIII HapII HhaI
Hin1I HinP1I HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PshBI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII