Restriction map for
pF1KA1254
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# NO CUT ENZYMES ( 130 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AgeI AhdI Alw44I AlwI AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AspI AvaI
AviII AvrII BamHI BbeI BbrPI
BbvCI BcgI BcnI BglII BlnI
BlpI Bpu1102I BsaAI BsaWI BseAI
BsgI BsiEI BsiWI BsmBI BsmI
BsoBI Bsp1407I BspDI BspEI BspMI
BsrBI BsrGI BssHII BssSI BstAPI
BstEII BstPI BstUI BstYI Bsu36I
CelII CfoI ClaI CpoI DraIII
DrdI DsaI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRV
EcoT22I FseI FspI HaeII HhaI
HinP1I HindIII HpaI KasI KpnI
KspI MflI MluI MroI MvnI
NaeI NarI NciI NcoI NgoMIV
NheI NotI NruI NsiI PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshBI Psp1406I
PstI PvuI RsrII SacI SacII
SalI SbfI SexAI SfiI SfoI
SgrAI SmaI SnaBI SphI Sse8387I
SspBI StuI SwaI Tth111I Van91I
XbaI XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AccI 429 GTATAC GT^[AC][GT]AC
AciI 113 CCGC CCGC(-3/-1)
AcyI 530 GACGCC G[GA]^CG[CT]C
AflII 1060 CTTAAG C^TTAAG
AflIII 1988 ACATGT A^C[GA][CT]GT
AspHI 1904 GTGCTC G[AT]GC[AT]^C
AvaII 2227 GGACC G^G[AT]CC
BalI 693 TGGCCA TGG^CCA
BanI 1725 GGCACC G^G[CT][GA]CC
BfrI 1060 CTTAAG C^TTAAG
BglI 182 GCCAAAATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 672 GATTGCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 530 GACGCC G[GA]^CG[CT]C
BsaI 640 GGTCTC GGTCTC(1/5)
BseRI 142 GAGGAG GAGGAG(10/8)
BsiHKAI 1904 GTGCTC G[AT]GC[AT]^C
BsmFI 1024 GGGAC GGGAC(10/14)
BspHI 54 TCATGA T^CATGA
BspLU11I 1988 ACATGT A^CATGT
BsrDI 155 CATTGC GCAATG(2/0)
BsrFI 575 ACCGGC [GA]^CCGG[CT]
Bst1107I 429 GTATAC GTA^TAC
BstBI 1318 TTCGAA TT^CGAA
BstXI 696 CCATTTCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 429 GTATAC GTA^TAC
Cfr10I 575 ACCGGC [GA]^CCGG[CT]
DraII 1752 AGGCCCT [GA]G^G[ACGT]CC[CT]
EaeI 693 TGGCCA [CT]^GGCC[GA]
EcoNI 939 CCTGGAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 1752 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoT14I 1499 CCAAGG C^C[AT][AT]GG
FauI 112 CCCGC CCCGC(4/6)
HapII 576 CCGG C^CGG
Hin1I 530 GACGCC G[GA]^CG[CT]C
HpaII 576 CCGG C^CGG
MaeII 624 ACGT A^CGT
MamI 672 GATTGCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 245 CAATTG C^AATTG
MluNI 693 TGGCCA TGG^CCA
MscI 693 TGGCCA TGG^CCA
MspI 576 CCGG C^CGG
MunI 245 CAATTG C^AATTG
NdeI 2222 CATATG CA^TATG
NspI 1988 ACATGT [GA]CATG^[CT]
NspV 1318 TTCGAA TT^CGAA
PshAI 2228 GACCCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 54 TCATGA T^CATGA
ScaI 1030 AGTACT AGT^ACT
SfuI 1318 TTCGAA TT^CGAA
SpeI 1170 ACTAGT A^CTAGT
SspI 1008 AATATT AAT^ATT
StyI 1499 CCAAGG C^C[AT][AT]GG
TaqI 1319 TCGA T^CGA
TthHB8I 1319 TCGA T^CGA
XcmI 1200 CCAGCAGTTAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AfaI 1031 GTAC GT^AC
1986 GTAC
AlwNI 446 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG
563 CAGAATCTG
Asp700I 1566 GAAAAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2082 GAAGCTCTTC
BanII 109 GAGCCC G[GA]GC[CT]^C
408 GGGCTC
BciVI 2004 GGATAC GTATCC(6/5)
934 GTATCC
BclI 2031 TGATCA T^GATCA
2076 TGATCA
BsiYI 939 CCTGGAGAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
2218 CCTCCATATGG
BslI 939 CCTGGAGAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
2218 CCTCCATATGG
BsmAI 1117 GAGAC GTCTC(1/5)
641 GTCTC
Cac8I 111 GCCCGC GC[ACGT]^[ACGT]GC
1536 GCAGGC
Cfr13I 1753 GGCCC G^G[ACGT]CC
2227 GGACC
DraI 1606 TTTAAA TTT^AAA
1798 TTTAAA
FbaI 2031 TGATCA T^GATCA
2076 TGATCA
HaeIII 694 GGCC GG^CC
1753 GGCC
HgaI 530 GACGC GACGC(5/10)
726 GCGTC
MspA1I 102 CAGCTG C[AC]G^C[GT]G
1673 CAGCTG
NlaIV 1725 GGCACC GG[ACGT]^[ACGT]CC
2227 GGACCC
PvuII 102 CAGCTG CAG^CTG
1673 CAGCTG
RsaI 1031 GTAC GT^AC
1986 GTAC
SapI 410 GCTCTTC GCTCTTC(1/4)
2085 GCTCTTC
Sau96I 1753 GGCCC G^G[ACGT]CC
2227 GGACC
SfcI 911 CTACAG C^T[GA][CT]AG
1730 CTACAG
SmlI 325 CTTGAG C^T[CT][GA]AG
1060 CTTAAG
XmnI 1566 GAAAAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2082 GAAGCTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
#########################################
AcsI AluI ApoI BbsI BbvI
BfaI BmyI BpmI BpuAI BsaJI
Bsp1286I BsrI BssKI BstF5I BstNI
DdeI DpnII EarI EcoRI EcoRII
Fnu4HI FokI HincII HindII HinfI
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MnlI MseI MslI
MvaI MwoI NdeII NlaIII PleI
Sau3AI ScrFI SfaNI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI