Restriction map for
pF1KA1191
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# NO CUT ENZYMES ( 145 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaLI AscI
AseI AsnI Asp718I AspEI AviII
AvrII BalI BamHI BanI BbeI
BbrPI BbsI BbvCI BciVI BfrI
BglII BlnI BlpI BpmI Bpu1102I
BpuAI BsaHI BsaWI BseAI BsgI
BsiEI BsiWI BsmBI Bsp1407I BspDI
BspEI BspLU11I BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRV EcoT22I FauI FseI
FspI HaeII Hin1I HincII HindII
HindIII HpaI KasI KpnI KspI
MfeI MluI MluNI MroI MscI
MseI MspA1I MunI MvnI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI PvuII
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI Tru9I XbaI XmaCI XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccI 227 GTCTAC GT^[AC][GT]AC
AcsI 318 GAATTC [GA]^AATT[CT]
AlwNI 564 CAGGGGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 863 GGGCCC GGGCC^C
ApoI 318 GAATTC [GA]^AATT[CT]
Asp700I 346 GAAAGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 895 GTGCTC G[AT]GC[AT]^C
AvaI 109 CTCGAG C^[CT]CG[GA]G
BcgI 111 CGAGGACCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 326 TGATCA T^GATCA
BcnI 84 CCGGG CC^[GC]GG
BglI 718 GCCACCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 767 TACGTG [CT]AC^GT[GA]
BsaBI 822 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 424 GAGACC GGTCTC(1/5)
BsiHKAI 895 GTGCTC G[AT]GC[AT]^C
BsmAI 424 GAGAC GTCTC(1/5)
BsmI 350 GCATTC GAATGC(1/-1)
BsoBI 109 CTCGAG C^[CT]CG[GA]G
BspHI 314 TCATGA T^CATGA
BstF5I 828 CATCC GGATG(2/0)
BstXI 779 CCAACCGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EaeI 443 TGGCCG [CT]^GGCC[GA]
EcoNI 198 CCTCGCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 318 GAATTC G^AATTC
FbaI 326 TGATCA T^GATCA
FokI 828 CATCC GGATG(9/13)
HapII 84 CCGG C^CGG
HgaI 155 GCGTC GACGC(5/10)
HpaII 84 CCGG C^CGG
MaeII 768 ACGT A^CGT
MamI 822 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI 828 CATCCCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 84 CCGG C^CGG
NciI 84 CCGGG CC^[GC]GG
NcoI 620 CCATGG C^CATGG
PaeR7I 109 CTCGAG C^TCGAG
PflMI 563 CCAGGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 314 TCATGA T^CATGA
StuI 725 AGGCCT AGG^CCT
Tsp45I 884 GTGAC ^GT[GC]AC
Tsp509I 319 AATT ^AATT
Van91I 563 CCAGGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 260 CCATTGACAGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 109 CTCGAG C^TCGAG
XmnI 346 GAAAGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AciI 81 CCGC CCGC(-3/-1)
538 CCGC
AfaI 432 GTAC GT^AC
699 GTAC
AluI 94 AGCT AG^CT
300 AGCT
AlwI 686 GATCC GGATC(4/5)
796 GATCC
AspI 246 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
274 GACAAGGTC
BanII 737 GAGCCC G[GA]GC[CT]^C
863 GGGCCC
BstYI 685 AGATCC [GA]^GATC[CT]
795 AGATCC
DdeI 379 CTGAG C^T[ACGT]AG
551 CTAAG
DsaI 620 CCATGG C^C[GA][CT]GG
859 CCACGG
EarI 543 CTCTTC CTCTTC(1/4)
493 GAAGAG
Ksp632I 543 CTCTTC CTCTTC(1/4)
493 GAAGAG
MflI 685 AGATCC [GA]^GATC[CT]
795 AGATCC
PflFI 246 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
274 GACAAGGTC
PleI 129 GACTC GAGTC(4/5)
625 GACTC
PpuMI 113 AGGACCC [GA]G^G[AT]CC[CT]
278 AGGTCCC
RsaI 432 GTAC GT^AC
699 GTAC
SfaNI 385 GATGC GCATC(5/9)
508 GCATC
SmlI 31 CTTGAG C^T[CT][GA]AG
109 CTCGAG
TaqI 110 TCGA T^CGA
438 TCGA
TfiI 174 GATTC G^A[AT]TC
703 GATTC
Tth111I 246 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
274 GACAAGGTC
TthHB8I 110 TCGA T^CGA
438 TCGA
XhoII 685 AGATCC [GA]^GATC[CT]
795 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 44 enzymes) #
#########################################
AvaII BbvI BfaI BmyI BsaJI
BseRI BsiYI BslI BsmFI Bsp1286I
BsrI BssKI BstNI Cac8I CfoI
Cfr13I DpnII DraII EcoO109I EcoRII
EcoT14I Fnu4HI HaeIII HhaI HinP1I
HinfI HphI ItaI MaeI MaeIII
MboI MboII MnlI MvaI MwoI
NdeII NlaIII NlaIV Sau3AI Sau96I
ScrFI StyI TseI TspRI