Restriction map for
pF1KA1160
##################################
# NO CUT ENZYMES ( 128 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AcyI AflII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AvaI AviII AvrII BalI
BamHI BbeI BbvCI BcgI BciVI
BclI BcnI BfrI BlnI BsaHI
BsgI BsiWI BsoBI Bsp1407I BspLU11I
BspMI BsrGI BssHII BssSI Bst1107I
BstAPI BstUI BstXI BstZ17I Bsu36I
CfoI CpoI DraI DraIII DrdI
DsaI Eam1105I Eco47III Eco81I EcoNI
EcoRV EcoT14I EcoT22I FauI FbaI
FspI HaeII HgaI HhaI Hin1I
HinP1I HincII HindII HindIII HpaI
KasI KpnI KspI MfeI MluI
MluNI MscI MslI MunI MvnI
NarI NciI NcoI NdeI NheI
NotI NruI NsiI NspI PacI
PaeR7I PflFI PflMI PinAI PmeI
PshAI PshBI Psp1406I PvuI RsrII
SacII SalI SapI SexAI SfaNI
SfoI SgrAI SmaI SmlI SnaBI
SpeI SphI SspBI SspI StuI
StyI SwaI Tth111I Van91I XbaI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
AccIII 266 TCCGGA T^CCGGA
AflIII 439 ACACGT A^C[GA][CT]GT
AlwI 321 GGATC GGATC(4/5)
Asp700I 415 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BanI 971 GGTGCC G^G[CT][GA]CC
BbrPI 440 CACGTG CAC^GTG
BbsI 83 GAAGAC GAAGAC(2/6)
BfaI 487 CTAG C^TAG
BpmI 790 CTCCAG CTGGAG(16/14)
BpuAI 83 GAAGAC GAAGAC(2/6)
BsaAI 440 CACGTG [CT]AC^GT[GA]
BsaBI 633 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 808 GAGACC GGTCTC(1/5)
BsaWI 266 TCCGGA [AT]^CCGG[AT]
BseAI 266 TCCGGA T^CCGGA
BsiEI 868 CGGCCG CG[GA][CT]^CG
BsmBI 135 GAGACG CGTCTC(1/5)
BsmI 183 GAATGC GAATGC(1/-1)
BspDI 463 ATCGAT AT^CGAT
BspEI 266 TCCGGA T^CCGGA
BsrBI 879 GAGCGG CCGCTC(-3/-3)
BsrDI 717 CATTGC GCAATG(2/0)
BsrFI 870 GCCGGC [GA]^CCGG[CT]
BstBI 203 TTCGAA TT^CGAA
BstEII 481 GGTTACC G^GT[ACGT]ACC
BstPI 481 GGTTACC G^GT[ACGT]ACC
Cfr10I 870 GCCGGC [GA]^CCGG[CT]
ClaI 463 ATCGAT AT^CGAT
DraII 285 AGGTCCT [GA]G^G[ACGT]CC[CT]
EagI 868 CGGCCG C^GGCCG
EclXI 868 CGGCCG C^GGCCG
Eco52I 868 CGGCCG C^GGCCG
EcoO109I 285 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 481 GGTTACC G^GT[ACGT]ACC
EcoRI 206 GAATTC G^AATTC
FseI 869 GGCCGGCC GGCCGG^CC
MaeI 487 CTAG C^TAG
MamI 633 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 266 TCCGGA T^CCGGA
MseI 230 TTAA T^TAA
MspA1I 55 CAGCTG C[AC]G^C[GT]G
NaeI 870 GCCGGC GCC^GGC
NgoMIV 870 GCCGGC G^CCGGC
NspV 203 TTCGAA TT^CGAA
PleI 661 GAGTC GAGTC(4/5)
PmaCI 440 CACGTG CAC^GTG
PmlI 440 CACGTG CAC^GTG
PpuMI 285 AGGTCCT [GA]G^G[AT]CC[CT]
PvuII 55 CAGCTG CAG^CTG
SbfI 813 CCTGCAGG CCTGCA^GG
SfiI 21 GGCCATGACGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 203 TTCGAA TT^CGAA
Sse8387I 813 CCTGCAGG CCTGCA^GG
TfiI 177 GATTC G^A[AT]TC
Tru9I 230 TTAA T^TAA
XcmI 836 CCAGAAGGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 415 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AcsI 199 GAATTT [GA]^AATT[CT]
206 GAATTC
ApoI 199 GAATTT [GA]^AATT[CT]
206 GAATTC
AspHI 448 GAGCTC G[AT]GC[AT]^C
956 GAGCTC
AvaII 264 GGTCC G^G[AT]CC
286 GGTCC
BanII 448 GAGCTC G[GA]GC[CT]^C
956 GAGCTC
BglI 22 GCCATGACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
918 GCCAATGGGGC
BglII 155 AGATCT A^GATCT
856 AGATCT
BlpI 52 GCTCAGC GC^T[ACGT]AGC
445 GCTGAGC
BmyI 448 GAGCTC G[AGT]GC[ACT]^C
956 GAGCTC
Bpu1102I 52 GCTCAGC GC^T[ACGT]AGC
445 GCTGAGC
BsiHKAI 448 GAGCTC G[AT]GC[AT]^C
956 GAGCTC
BsiYI 267 CCGGATAAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
289 CCTGATTATGG
BslI 267 CCGGATAAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
289 CCTGATTATGG
BsmAI 135 GAGAC GTCTC(1/5)
808 GAGAC
Bsp1286I 448 GAGCTC G[AGT]GC[ACT]^C
956 GAGCTC
BspHI 324 TCATGA T^CATGA
452 TCATGA
BstF5I 929 CATCC GGATG(2/0)
989 GGATG
BstYI 155 AGATCT [GA]^GATC[CT]
856 AGATCT
CelII 52 GCTCAGC GC^T[ACGT]AGC
445 GCTGAGC
EaeI 868 CGGCCG [CT]^GGCC[GA]
872 CGGCCA
FokI 929 CATCC GGATG(9/13)
989 GGATG
HapII 267 CCGG C^CGG
871 CCGG
HinfI 177 GATTC G^A[ACGT]TC
661 GAGTC
HpaII 267 CCGG C^CGG
871 CCGG
MaeII 441 ACGT A^CGT
725 ACGT
MflI 155 AGATCT [GA]^GATC[CT]
856 AGATCT
MspI 267 CCGG C^CGG
871 CCGG
PstI 814 CTGCAG CTGCA^G
852 CTGCAG
RcaI 324 TCATGA T^CATGA
452 TCATGA
SacI 448 GAGCTC GAGCT^C
956 GAGCTC
ScaI 362 AGTACT AGT^ACT
473 AGTACT
SfcI 814 CTGCAG C^T[GA][CT]AG
852 CTGCAG
TaqI 204 TCGA T^CGA
464 TCGA
Tsp45I 212 GTGAC ^GT[GC]AC
649 GTCAC
TthHB8I 204 TCGA T^CGA
464 TCGA
XhoII 155 AGATCT [GA]^GATC[CT]
856 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 37 enzymes) #
#########################################
AciI AfaI AluI BbvI BsaJI
BseRI BsmFI BsrI BssKI BstNI
Cac8I Cfr13I DdeI DpnII EarI
EcoRII Fnu4HI HaeIII HphI ItaI
Ksp632I MaeIII MboI MboII MnlI
MvaI MwoI NdeII NlaIII NlaIV
RsaI Sau3AI Sau96I ScrFI TseI
Tsp509I TspRI