Restriction map for
pF1KA1153
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# NO CUT ENZYMES ( 151 enzymes) #
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AatII Acc65I AccIII AclI AcyI
AflII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaLI AscI AseI
AsnI Asp718I AspEI AspHI AspI
AvaI AviII AvrII BamHI BanI
BbeI BbrPI BbvCI BcgI BciVI
BclI BfrI BglI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsaHI
BsaWI BseAI BsiEI BsiHKAI BsiWI
BsmBI BsmFI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstEII BstPI BstZ17I Bsu36I CelII
CfoI Cfr10I ClaI CpoI DraIII
DrdI DsaI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRV
EcoT14I EcoT22I FbaI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HpaI HphI KasI KpnI KspI
MamI MfeI MluI MroI MunI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsrII SacI
SacII SalI SbfI ScaI SexAI
SfiI SfoI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI TfiI Tth111I
Van91I XbaI XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccI 218 GTCTAC GT^[AC][GT]AC
AccII 61 CGCG CG^CG
AflIII 622 ACGTGT A^C[GA][CT]GT
AlwI 979 GATCC GGATC(4/5)
ApaI 24 GGGCCC GGGCC^C
AvaII 703 GGACC G^G[AT]CC
BalI 174 TGGCCA TGG^CCA
BfaI 562 CTAG C^TAG
BsaI 47 GAGACC GGTCTC(1/5)
BsaJI 693 CCCTGG C^C[ACGT][ACGT]GG
BsgI 626 GTGCAG GTGCAG(16/14)
BsiYI 694 CCTGGAGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 694 CCTGGAGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmI 1176 GCATTC GAATGC(1/-1)
BsrBI 1082 CCGCTC CCGCTC(-3/-3)
BsrDI 646 GCAATG GCAATG(2/0)
BstBI 139 TTCGAA TT^CGAA
BstUI 61 CGCG CG^CG
BstXI 177 CCATAGTTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 978 AGATCC [GA]^GATC[CT]
DpnII 979 GATC ^GATC
DraI 100 TTTAAA TTT^AAA
EaeI 174 TGGCCA [CT]^GGCC[GA]
EcoNI 694 CCTGGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 766 GAATTC G^AATTC
FauI 268 GCGGG CCCGC(4/6)
HincII 573 GTTGAC GT[CT]^[GA]AC
HindII 573 GTTGAC GT[CT]^[GA]AC
HindIII 335 AAGCTT A^AGCTT
HinfI 1137 GACTC G^A[ACGT]TC
MaeI 562 CTAG C^TAG
MboI 979 GATC ^GATC
MflI 978 AGATCC [GA]^GATC[CT]
MluNI 174 TGGCCA TGG^CCA
MscI 174 TGGCCA TGG^CCA
MslI 178 CATAGTTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 118 CAGCGG C[AC]G^C[GT]G
MvnI 61 CGCG CG^CG
MwoI 715 GCAGCAACAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeII 979 GATC ^GATC
NspV 139 TTCGAA TT^CGAA
PleI 1137 GACTC GAGTC(4/5)
PpuMI 702 AGGACCT [GA]G^G[AT]CC[CT]
PstI 251 CTGCAG CTGCA^G
Sau3AI 979 GATC ^GATC
SfuI 139 TTCGAA TT^CGAA
Tsp45I 202 GTGAC ^GT[GC]AC
XhoII 978 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 22 enzymes) #
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AcsI 418 GAATTT [GA]^AATT[CT]
766 GAATTC
AfaI 109 GTAC GT^AC
395 GTAC
AluI 336 AGCT AG^CT
686 AGCT
ApoI 418 GAATTT [GA]^AATT[CT]
766 GAATTC
Asp700I 367 GAAATAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
803 GAACATTTTC
BcnI 22 CCGGG CC^[GC]GG
43 CCCGG
BpmI 695 CTGGAG CTGGAG(16/14)
874 CTGGAG
BsrI 873 ACTGG ACTGG(1/-1)
206 CCAGT
HapII 22 CCGG C^CGG
44 CCGG
HpaII 22 CCGG C^CGG
44 CCGG
MaeIII 133 GTAAC ^GT[ACGT]AC
202 GTGAC
MspI 22 CCGG C^CGG
44 CCGG
NciI 22 CCGGG CC^[GC]GG
43 CCCGG
NspI 724 GCATGT [GA]CATG^[CT]
1148 GCATGT
RsaI 109 GTAC GT^AC
395 GTAC
SapI 241 GAAGAGC GCTCTTC(1/4)
1051 GAAGAGC
SfaNI 39 GCATC GCATC(5/9)
56 GCATC
SfcI 220 CTACAG C^T[GA][CT]AG
251 CTGCAG
SmlI 320 CTCAAG C^T[CT][GA]AG
974 CTCAAG
TaqI 140 TCGA T^CGA
282 TCGA
TthHB8I 140 TCGA T^CGA
282 TCGA
XmnI 367 GAAATAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
803 GAACATTTTC
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# 3 OR MORE CUT ENZYMES ( 37 enzymes) #
#########################################
AciI BanII BbsI BbvI BmyI
BpuAI BseRI BsmAI Bsp1286I BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DraII EarI EcoO109I EcoRII Fnu4HI
FokI HaeIII ItaI Ksp632I MaeII
MboII MnlI MseI MvaI NlaIII
NlaIV Sau96I ScrFI Tru9I TseI
Tsp509I TspRI