Restriction map for
pF1KA1147
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# NO CUT ENZYMES ( 102 enzymes) #
##################################
Acc65I AccIII AflII AgeI AhdI
Alw44I AocI Aor51HI ApaLI AscI
AseI AsnI Asp718I AspEI AspI
AviII AvrII BamHI BbrPI BbvCI
BcgI BciVI BclI BfrI BglII
BlnI BlpI Bpu1102I BseAI BsiWI
BsmI Bsp1407I BspDI BspEI BspHI
BsrDI BsrGI BssSI Bst1107I BstXI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DraIII DrdI Eam1105I Eco47III
Eco81I EcoRI EcoRV EcoT22I FbaI
FspI HincII HindII HindIII HpaI
KpnI MfeI MluI MroI MseI
MunI NcoI NdeI NheI NotI
NruI NsiI PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI PvuI RcaI RsrII SacI
SalI SapI SbfI ScaI SfiI
SgrAI SnaBI SpeI Sse8387I SspBI
SspI SwaI TfiI Tru9I Tth111I
XbaI XhoI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AatII 994 GACGTC GACGT^C
AclI 844 AACGTT AA^CGTT
AcsI 751 AAATTT [GA]^AATT[CT]
AflIII 662 ACATGT A^C[GA][CT]GT
AlwI 735 GATCC GGATC(4/5)
ApoI 751 AAATTT [GA]^AATT[CT]
Asp700I 963 GAAGATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 592 GTGCTC G[AT]GC[AT]^C
BsaI 1286 GAGACC GGTCTC(1/5)
BsgI 951 CTGCAC GTGCAG(16/14)
BsiHKAI 592 GTGCTC G[AT]GC[AT]^C
BspLU11I 662 ACATGT A^CATGT
BsrBI 261 CCGCTC CCGCTC(-3/-3)
BssHII 443 GCGCGC G^CGCGC
BstBI 764 TTCGAA TT^CGAA
BstEII 246 GGTCACC G^GT[ACGT]ACC
BstPI 246 GGTCACC G^GT[ACGT]ACC
BstYI 734 AGATCC [GA]^GATC[CT]
EcoO65I 246 GGTCACC G^GT[ACGT]ACC
EcoT14I 1281 CCAAGG C^C[AT][AT]GG
FseI 122 GGCCGGCC GGCCGG^CC
HgaI 16 GACGC GACGC(5/10)
HinfI 880 GAGTC G^A[ACGT]TC
HphI 248 TCACC GGTGA(8/7)
KspI 128 CCGCGG CCGC^GG
MaeIII 247 GTCAC ^GT[ACGT]AC
MflI 734 AGATCC [GA]^GATC[CT]
MslI 414 CATGCCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NspV 764 TTCGAA TT^CGAA
PflMI 225 CCAGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 880 GAGTC GAGTC(4/5)
Psp1406I 844 AACGTT AA^CGTT
PvuII 692 CAGCTG CAG^CTG
SacII 128 CCGCGG CCGC^GG
SfuI 764 TTCGAA TT^CGAA
SmlI 293 CTCAAG C^T[CT][GA]AG
SphI 1254 GCATGC GCATG^C
StuI 1316 AGGCCT AGG^CCT
StyI 1281 CCAAGG C^C[AT][AT]GG
Tsp45I 247 GTCAC ^GT[GC]AC
Tsp509I 752 AATT ^AATT
Van91I 225 CCAGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 410 CCAACATGCCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 734 AGATCC [GA]^GATC[CT]
XmnI 963 GAAGATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccI 634 GTCTAC GT^[AC][GT]AC
997 GTCTAC
AlwNI 1042 CAGCCGCTG CAG[ACGT][ACGT][ACGT]^CTG
1105 CAGATGCTG
ApaI 45 GGGCCC GGGCC^C
382 GGGCCC
BalI 332 TGGCCA TGG^CCA
839 TGGCCA
BbsI 1020 GAAGAC GAAGAC(2/6)
1135 GAAGAC
BfaI 1180 CTAG C^TAG
1273 CTAG
BpuAI 1020 GAAGAC GAAGAC(2/6)
1135 GAAGAC
BsaAI 898 TACGTG [CT]AC^GT[GA]
1000 TACGTG
BsaBI 675 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
735 GATCCTCATC
BsaWI 1193 ACCGGA [AT]^CCGG[AT]
1289 ACCGGA
BsmBI 54 CGTCTC CGTCTC(1/5)
14 GAGACG
BsmFI 342 GTCCC GGGAC(10/14)
605 GTCCC
BspMI 1037 ACCTGC ACCTGC(4/8)
1307 ACCTGC
BstAPI 1041 GCAGCCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1249 GCAGAGCATGC
DsaI 128 CCGCGG C^C[GA][CT]GG
419 CCGTGG
EarI 1162 CTCTTC CTCTTC(1/4)
1153 GAAGAG
EcoNI 60 CCTGCCGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1308 CCTGCTGGAGG
Ksp632I 1162 CTCTTC CTCTTC(1/4)
1153 GAAGAG
MaeI 1180 CTAG C^TAG
1273 CTAG
MamI 675 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
735 GATCCTCATC
MluNI 332 TGGCCA TGG^CCA
839 TGGCCA
MscI 332 TGGCCA TGG^CCA
839 TGGCCA
PpuMI 340 GGGTCCC [GA]G^G[AT]CC[CT]
1157 AGGACCT
PstI 184 CTGCAG CTGCA^G
1039 CTGCAG
SexAI 224 ACCAGGT A^CC[AT]GGT
518 ACCAGGT
SmaI 190 CCCGGG CCC^GGG
1259 CCCGGG
XmaCI 190 CCCGGG C^CCGGG
1259 CCCGGG
XmaI 190 CCCGGG C^CCGGG
1259 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 83 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AvaI AvaII BanI BanII BbeI
BbvI BcnI BglI BmyI BpmI
BsaHI BsaJI BseRI BsiEI BsiYI
BslI BsmAI BsoBI Bsp1286I BsrFI
BsrI BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII EaeI EagI EclXI
Eco52I EcoO109I EcoRII FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
Hin1I HinP1I HpaII ItaI KasI
MaeII MboI MboII MnlI MspA1I
MspI MvaI MvnI MwoI NaeI
NarI NciI NdeII NgoMIV NlaIII
NlaIV NspI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SfoI TaqI
TseI TspRI TthHB8I