Restriction map for
pF1KA1145
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# NO CUT ENZYMES ( 121 enzymes) #
##################################
AatII Acc65I AccI AclI AcyI
AfaI AflIII AgeI AhdI Alw44I
AocI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaI AviII AvrII BamHI BbeI
BbsI BclI BglII BlnI BpuAI
BsaHI BsiWI BsoBI Bsp1407I BspDI
BspLU11I BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstYI BstZ17I Bsu36I Cfr10I ClaI
CpoI DraI DraIII DrdI DsaI
Eam1105I Eco81I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HgaI
Hin1I HincII HindII HindIII HpaI
KasI KpnI KspI MfeI MflI
MluI MunI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NsiI
NspV PacI PaeR7I PflFI PinAI
PmeI PpuMI PshAI PshBI Psp1406I
PvuI RsaI RsrII SacII SalI
SapI SbfI ScaI SexAI SfoI
SfuI SgrAI SmaI SnaBI Sse8387I
SspBI SspI SwaI Tth111I XbaI
XcmI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccIII 536 TCCGGA T^CCGGA
AflII 9 CTTAAG C^TTAAG
BalI 980 TGGCCA TGG^CCA
BanI 1249 GGCACC G^G[CT][GA]CC
BbrPI 418 CACGTG CAC^GTG
BbvCI 820 GCTGAGG CCTCAGC(-5/-2)
BciVI 801 GGATAC GTATCC(6/5)
BcnI 69 CCCGG CC^[GC]GG
BfrI 9 CTTAAG C^TTAAG
BglI 1003 GCCTACCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 811 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 811 GCTCAGC GC^T[ACGT]AGC
BsaAI 418 CACGTG [CT]AC^GT[GA]
BsaBI 391 GATATACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 477 GGTCTC GGTCTC(1/5)
BsaWI 536 TCCGGA [AT]^CCGG[AT]
BseAI 536 TCCGGA T^CCGGA
BsgI 844 GTGCAG GTGCAG(16/14)
BsiEI 698 CGGCCG CG[GA][CT]^CG
BsmBI 1212 CGTCTC CGTCTC(1/5)
BsmI 1122 GAATGC GAATGC(1/-1)
BspEI 536 TCCGGA T^CCGGA
BspHI 1169 TCATGA T^CATGA
BspMI 941 ACCTGC ACCTGC(4/8)
BstAPI 1098 GCAAGCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 811 GCTCAGC GC^T[ACGT]AGC
DraII 616 AGGGCCT [GA]G^G[ACGT]CC[CT]
EagI 698 CGGCCG C^GGCCG
EclXI 698 CGGCCG C^GGCCG
Eco52I 698 CGGCCG C^GGCCG
EcoO109I 616 AGGGCCT [GA]G^G[ACGT]CC[CT]
EcoT14I 1043 CCTTGG C^C[AT][AT]GG
HphI 1216 TCACC GGTGA(8/7)
MamI 391 GATATACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 980 TGGCCA TGG^CCA
MroI 536 TCCGGA T^CCGGA
MscI 980 TGGCCA TGG^CCA
NciI 69 CCCGG CC^[GC]GG
NruI 1202 TCGCGA TCG^CGA
NspI 467 GCATGC [GA]CATG^[CT]
PflMI 441 CCATTGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 418 CACGTG CAC^GTG
PmlI 418 CACGTG CAC^GTG
RcaI 1169 TCATGA T^CATGA
SfiI 1002 GGCCTACCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 9 CTTAAG C^T[CT][GA]AG
SpeI 201 ACTAGT A^CTAGT
SphI 467 GCATGC GCATG^C
StuI 1010 AGGCCT AGG^CCT
StyI 1043 CCTTGG C^C[AT][AT]GG
Tsp45I 1264 GTGAC ^GT[GC]AC
Van91I 441 CCATTGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AcsI 128 AAATTC [GA]^AATT[CT]
579 AAATTC
AlwNI 877 CAGACCCTG CAG[ACGT][ACGT][ACGT]^CTG
973 CAGGAGCTG
Aor51HI 631 AGCGCT AGC^GCT
1019 AGCGCT
ApoI 128 AAATTC [GA]^AATT[CT]
579 AAATTC
AspHI 357 GAGCTC G[AT]GC[AT]^C
1078 GAGCTC
BanII 357 GAGCTC G[GA]GC[CT]^C
1078 GAGCTC
BcgI 558 CGACAACATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
309 GCAGTATCATCG
BfaI 202 CTAG C^TAG
353 CTAG
BmyI 357 GAGCTC G[AGT]GC[ACT]^C
1078 GAGCTC
BseRI 589 GAGGAG GAGGAG(10/8)
991 GAGGAG
BsiHKAI 357 GAGCTC G[AT]GC[AT]^C
1078 GAGCTC
Bsp1286I 357 GAGCTC G[AGT]GC[ACT]^C
1078 GAGCTC
BsrDI 442 CATTGC GCAATG(2/0)
564 CATTGC
EaeI 698 CGGCCG [CT]^GGCC[GA]
980 TGGCCA
EarI 1268 CTCTTC CTCTTC(1/4)
1227 GAAGAG
Eco47III 631 AGCGCT AGC^GCT
1019 AGCGCT
EcoNI 882 CCTGCAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
966 CCTGAAGCAGG
FauI 229 GCGGG CCCGC(4/6)
1026 GCGGG
HapII 70 CCGG C^CGG
537 CCGG
HpaII 70 CCGG C^CGG
537 CCGG
Ksp632I 1268 CTCTTC CTCTTC(1/4)
1227 GAAGAG
MaeI 202 CTAG C^TAG
353 CTAG
MseI 10 TTAA T^TAA
851 TTAA
MspI 70 CCGG C^CGG
537 CCGG
SacI 357 GAGCTC GAGCT^C
1078 GAGCTC
TfiI 261 GAATC G^A[AT]TC
1048 GAATC
Tru9I 10 TTAA T^TAA
851 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AccII AciI AluI AlwI AvaII
BbvI BpmI BsaJI BsiYI BslI
BsmAI BsmFI BsrI BssKI BstF5I
BstNI BstUI BstXI Cac8I CfoI
Cfr13I DdeI DpnII EcoRII Fnu4HI
FokI HaeII HaeIII HhaI HinP1I
HinfI ItaI MaeII MaeIII MboI
MboII MnlI MslI MspA1I MvaI
MvnI MwoI NdeII NlaIII NlaIV
PleI PstI PvuII Sau3AI Sau96I
ScrFI SfaNI SfcI TaqI TseI
Tsp509I TspRI TthHB8I