Restriction map for
pF1KA1144
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# NO CUT ENZYMES ( 107 enzymes) #
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Acc65I AccI AccIII AclI AcsI
AflII AgeI Alw44I Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I BamHI BbrPI BbvCI
BcgI BciVI BclI BfrI BglI
BglII BsaI BsaWI BseAI BsgI
BsiWI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrGI BssHII Bst1107I
BstEII BstPI BstZ17I ClaI CpoI
DrdI EagI EclXI Eco47III Eco52I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI HincII HindII HpaI
KpnI KspI MfeI MluI MroI
MunI NaeI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI
PaeR7I PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SbfI
SfiI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Van91I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AatII 190 GACGTC GACGT^C
AflIII 23 ACGTGT A^C[GA][CT]GT
AhdI 790 GACCTCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 888 CCTGAGG CC^T[ACGT]AGG
AspEI 790 GACCTCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 187 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1280 TGCGCA TGC^GCA
AvrII 673 CCTAGG C^CTAGG
BanI 1253 GGCGCC G^G[CT][GA]CC
BbeI 1253 GGCGCC GGCGC^C
BcnI 538 CCCGG CC^[GC]GG
BfaI 674 CTAG C^TAG
BlnI 673 CCTAGG C^CTAGG
BpmI 166 CTGGAG CTGGAG(16/14)
BsaAI 238 TACGTG [CT]AC^GT[GA]
BsiEI 213 CGACCG CG[GA][CT]^CG
BsmAI 118 GAGAC GTCTC(1/5)
BsmBI 118 GAGACG CGTCTC(1/5)
BsmI 771 GAATGC GAATGC(1/-1)
BsrBI 945 CCGCTC CCGCTC(-3/-3)
BsrDI 702 CATTGC GCAATG(2/0)
BssSI 1198 CTCGTG CACGAG(-5/-1)
BstAPI 96 GCACACGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 679 TTCGAA TT^CGAA
BstXI 578 CCATCCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 888 CCTGAGG CC^T[ACGT]AGG
DraI 1413 TTTAAA TTT^AAA
Eam1105I 790 GACCTCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 229 CTCTTC CTCTTC(1/4)
Eco81I 888 CCTGAGG CC^T[ACGT]AGG
FauI 198 GCGGG CCCGC(4/6)
FspI 1280 TGCGCA TGC^GCA
HindIII 265 AAGCTT A^AGCTT
KasI 1253 GGCGCC G^GCGCC
Ksp632I 229 CTCTTC CTCTTC(1/4)
MaeI 674 CTAG C^TAG
NarI 1253 GGCGCC GG^CGCC
NciI 538 CCCGG CC^[GC]GG
NcoI 369 CCATGG C^CATGG
NspV 679 TTCGAA TT^CGAA
PflFI 187 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 346 GACTC GAGTC(4/5)
PstI 354 CTGCAG CTGCA^G
SapI 228 GCTCTTC GCTCTTC(1/4)
ScaI 317 AGTACT AGT^ACT
SexAI 833 ACCTGGT A^CC[AT]GGT
SfoI 1253 GGCGCC GGC^GCC
SfuI 679 TTCGAA TT^CGAA
TfiI 1420 GATTC G^A[AT]TC
Tsp509I 1327 AATT ^AATT
Tth111I 187 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AfaI 318 GTAC GT^AC
1128 GTAC
AlwNI 10 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG
883 CAGGTCCTG
AvaI 115 CCCGAG C^[CT]CG[GA]G
388 CCCGAG
BlpI 280 GCTGAGC GC^T[ACGT]AGC
552 GCTGAGC
Bpu1102I 280 GCTGAGC GC^T[ACGT]AGC
552 GCTGAGC
BseRI 81 GAGGAG GAGGAG(10/8)
1057 GAGGAG
BsoBI 115 CCCGAG C^[CT]CG[GA]G
388 CCCGAG
BsrI 320 ACTGG ACTGG(1/-1)
937 ACTGG
CelII 280 GCTGAGC GC^T[ACGT]AGC
552 GCTGAGC
DraIII 606 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
1397 CACCAAGTG
EcoT14I 369 CCATGG C^C[AT][AT]GG
673 CCTAGG
HaeII 525 GGCGCT [GA]GCGC^[CT]
1253 GGCGCC
HgaI 120 GACGC GACGC(5/10)
469 GACGC
HinfI 346 GACTC G^A[ACGT]TC
1420 GATTC
MaeIII 416 GTGAC ^GT[ACGT]AC
1289 GTGAC
PvuII 514 CAGCTG CAG^CTG
1284 CAGCTG
RsaI 318 GTAC GT^AC
1128 GTAC
SfcI 354 CTGCAG C^T[GA][CT]AG
360 CTACAG
SmlI 757 CTCAAG C^T[CT][GA]AG
1267 CTTGAG
StyI 369 CCATGG C^C[AT][AT]GG
673 CCTAGG
Tsp45I 416 GTGAC ^GT[GC]AC
1289 GTGAC
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# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
#########################################
AccII AciI AcyI AluI AlwI
AspHI AvaII BalI BanII BbsI
BbvI BmyI BpuAI BsaBI BsaHI
BsaJI BsiHKAI BsiYI BslI BsmFI
Bsp1286I BsrFI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EcoO109I EcoRII Fnu4HI FokI
HaeIII HapII HhaI Hin1I HinP1I
HpaII HphI ItaI MaeII MamI
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NdeII NlaIII
NlaIV PpuMI SacI Sau3AI Sau96I
ScrFI SfaNI TaqI Tru9I TseI
TspRI TthHB8I XcmI XhoII