Restriction map for
pF1KA1141
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# NO CUT ENZYMES ( 111 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AcyI AflII AgeI AhdI AocI
Aor51HI ApaI AscI AseI AsnI
Asp718I AspEI AviII AvrII BamHI
BbeI BbrPI BcgI BclI BfrI
BglI BlnI BsaAI BsaHI BseAI
BsgI BsiEI BsiWI BsmBI BspDI
BspEI BspLU11I BspMI BsrBI BsrDI
BssHII Bst1107I BstAPI BstEII BstPI
BstUI BstZ17I Bsu36I CfoI ClaI
CpoI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRV EcoT22I FbaI FseI FspI
HaeII HhaI Hin1I HinP1I HpaI
KasI KpnI KspI MluI MroI
MspA1I MvnI NarI NheI NotI
NruI NsiI NspI PacI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI PvuI PvuII RsrII SacII
SalI SbfI SfiI SfoI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspI SwaI Van91I XcmI XmaCI
XmaI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AflIII 86 ACACGT A^C[GA][CT]GT
Alw44I 1973 GTGCAC G^TGCAC
AlwNI 2491 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 1973 GTGCAC G^TGCAC
BalI 1800 TGGCCA TGG^CCA
BciVI 762 GTATCC GTATCC(6/5)
BcnI 989 CCCGG CC^[GC]GG
BlpI 2227 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2227 GCTGAGC GC^T[ACGT]AGC
BsaBI 1492 GATAACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 235 GAGACC GGTCTC(1/5)
Bsp1407I 2586 TGTACA T^GTACA
BsrGI 2586 TGTACA T^GTACA
BstBI 268 TTCGAA TT^CGAA
BstXI 162 CCACCCAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 2227 GCTGAGC GC^T[ACGT]AGC
DraII 119 AGGACCT [GA]G^G[ACGT]CC[CT]
EaeI 1800 TGGCCA [CT]^GGCC[GA]
EcoNI 190 CCTCTGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 119 AGGACCT [GA]G^G[ACGT]CC[CT]
HgaI 1391 GCGTC GACGC(5/10)
HincII 2531 GTCAAC GT[CT]^[GA]AC
HindII 2531 GTCAAC GT[CT]^[GA]AC
MamI 1492 GATAACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 111 CAATTG C^AATTG
MluNI 1800 TGGCCA TGG^CCA
MscI 1800 TGGCCA TGG^CCA
MunI 111 CAATTG C^AATTG
NaeI 1406 GCCGGC GCC^GGC
NciI 989 CCCGG CC^[GC]GG
NcoI 503 CCATGG C^CATGG
NdeI 1541 CATATG CA^TATG
NgoMIV 1406 GCCGGC G^CCGGC
NspV 268 TTCGAA TT^CGAA
PaeR7I 2562 CTCGAG C^TCGAG
PpuMI 119 AGGACCT [GA]G^G[AT]CC[CT]
PstI 1716 CTGCAG CTGCA^G
SapI 1183 GCTCTTC GCTCTTC(1/4)
ScaI 1807 AGTACT AGT^ACT
SexAI 358 ACCTGGT A^CC[AT]GGT
SfuI 268 TTCGAA TT^CGAA
SspBI 2586 TGTACA T^GTACA
XbaI 1840 TCTAGA T^CTAGA
XhoI 2562 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AclI 2127 AACGTT AA^CGTT
2534 AACGTT
AspI 464 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2038 GACAGAGTC
AvaI 67 CTCGGG C^[CT]CG[GA]G
2562 CTCGAG
AvaII 120 GGACC G^G[AT]CC
135 GGACC
BanI 1010 GGCACC G^G[CT][GA]CC
2555 GGTGCC
BanII 2139 GAGCTC G[GA]GC[CT]^C
2307 GAGCTC
BbsI 392 GTCTTC GAAGAC(2/6)
2044 GTCTTC
BbvCI 2568 CCTCAGC CCTCAGC(-5/-2)
4 GCTGAGG
BglII 180 AGATCT A^GATCT
1646 AGATCT
BpuAI 392 GTCTTC GAAGAC(2/6)
2044 GTCTTC
BsaWI 740 ACCGGA [AT]^CCGG[AT]
2327 ACCGGA
BseRI 248 CTCCTC GAGGAG(10/8)
1198 GAGGAG
BsoBI 67 CTCGGG C^[CT]CG[GA]G
2562 CTCGAG
BspHI 447 TCATGA T^CATGA
906 TCATGA
BsrFI 1322 ACCGGC [GA]^CCGG[CT]
1406 GCCGGC
BssSI 1597 CACGAG CACGAG(-5/-1)
1738 CACGAG
Cac8I 590 GCCAGC GC[ACGT]^[ACGT]GC
1406 GCCGGC
Cfr10I 1322 ACCGGC [GA]^CCGG[CT]
1406 GCCGGC
DraI 1936 TTTAAA TTT^AAA
2020 TTTAAA
EarI 124 CTCTTC CTCTTC(1/4)
1184 CTCTTC
HindIII 648 AAGCTT A^AGCTT
2462 AAGCTT
Ksp632I 124 CTCTTC CTCTTC(1/4)
1184 CTCTTC
PflFI 464 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2038 GACAGAGTC
Psp1406I 2127 AACGTT AA^CGTT
2534 AACGTT
RcaI 447 TCATGA T^CATGA
906 TCATGA
SacI 2139 GAGCTC GAGCT^C
2307 GAGCTC
StuI 1313 AGGCCT AGG^CCT
2210 AGGCCT
TaqI 269 TCGA T^CGA
2563 TCGA
Tth111I 464 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2038 GACAGAGTC
TthHB8I 269 TCGA T^CGA
2563 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 73 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI Asp700I AspHI BbvI BfaI
BmyI BpmI BsaJI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
BsrI BssKI BstF5I BstNI BstYI
Cfr13I DdeI DpnII DsaI EcoRI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspI MvaI MwoI NdeII NlaIII
NlaIV PleI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI XhoII XmnI