Restriction map for
pF1KA1079
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# NO CUT ENZYMES ( 75 enzymes) #
##################################
AatII AccIII AclI AgeI AscI
AspI AvrII BalI BamHI BclI
BglI BglII BlnI BsaBI BseAI
BsiWI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrGI BssHII Bst1107I BstZ17I
ClaI CpoI EagI EarI EclXI
Eco52I EcoRI EcoRV EcoT22I FbaI
FseI HindIII HpaI Ksp632I MamI
MfeI MluI MluNI MroI MscI
MunI NcoI NdeI NheI NotI
NruI NsiI PacI PaeR7I PflFI
PinAI PmeI Psp1406I RcaI RsrII
SacI SapI SbfI ScaI SexAI
SfiI SnaBI SphI Sse8387I SspBI
StuI SwaI Tth111I XbaI XhoI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AcyI 108 GGCGCC G[GA]^CG[CT]C
AflII 2245 CTTAAG C^TTAAG
AhdI 2941 GACAGCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1207 GTGCAC G^TGCAC
ApaLI 1207 GTGCAC G^TGCAC
AseI 171 ATTAAT AT^TAAT
AsnI 171 ATTAAT AT^TAAT
Asp700I 616 GAAGCGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 2941 GACAGCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 677 TGCGCA TGC^GCA
BbeI 108 GGCGCC GGCGC^C
BciVI 196 GTATCC GTATCC(6/5)
BfrI 2245 CTTAAG C^TTAAG
BsaHI 108 GGCGCC G[GA]^CG[CT]C
BsaWI 1515 TCCGGT [AT]^CCGG[AT]
BsiEI 3657 CGATCG CG[GA][CT]^CG
BsmBI 3268 GAGACG CGTCTC(1/5)
BssSI 1500 CACGAG CACGAG(-5/-1)
BstBI 2458 TTCGAA TT^CGAA
BstEII 658 GGTGACC G^GT[ACGT]ACC
BstPI 658 GGTGACC G^GT[ACGT]ACC
DraIII 3946 CACCCCGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1249 GACTCAGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 2941 GACAGCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 658 GGTGACC G^GT[ACGT]ACC
EcoT14I 3380 CCTTGG C^C[AT][AT]GG
FspI 677 TGCGCA TGC^GCA
Hin1I 108 GGCGCC G[GA]^CG[CT]C
KasI 108 GGCGCC G^GCGCC
NarI 108 GGCGCC GG^CGCC
NspV 2458 TTCGAA TT^CGAA
PflMI 2628 CCACAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 2569 GACATCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 171 ATTAAT AT^TAAT
PvuI 3657 CGATCG CGAT^CG
SalI 1258 GTCGAC G^TCGAC
SfoI 108 GGCGCC GGC^GCC
SfuI 2458 TTCGAA TT^CGAA
SgrAI 11 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 3360 CCCGGG CCC^GGG
SpeI 3060 ACTAGT A^CTAGT
StyI 3380 CCTTGG C^C[AT][AT]GG
Van91I 2628 CCACAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 3360 CCCGGG C^CCGGG
XmaI 3360 CCCGGG C^CCGGG
XmnI 616 GAAGCGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 35 enzymes) #
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Acc65I 2442 GGTACC G^GTACC
2484 GGTACC
AccI 1258 GTCGAC GT^[AC][GT]AC
4090 GTCTAC
AcsI 232 GAATTT [GA]^AATT[CT]
2603 AAATTC
AflIII 1866 ACACGT A^C[GA][CT]GT
2527 ACGTGT
AocI 1590 CCTGAGG CC^T[ACGT]AGG
4181 CCTCAGG
Aor51HI 1707 AGCGCT AGC^GCT
3021 AGCGCT
ApoI 232 GAATTT [GA]^AATT[CT]
2603 AAATTC
Asp718I 2442 GGTACC G^GTACC
2484 GGTACC
BbrPI 1867 CACGTG CAC^GTG
2526 CACGTG
BbvCI 3915 CCTCAGC CCTCAGC(-5/-2)
3963 CCTCAGC
BcgI 1341 CGACCACTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3699 CGAACTCCTTGC
BlpI 1441 GCTAAGC GC^T[ACGT]AGC
4308 GCTCAGC
Bpu1102I 1441 GCTAAGC GC^T[ACGT]AGC
4308 GCTCAGC
BsaAI 1867 CACGTG [CT]AC^GT[GA]
2526 CACGTG
BsrBI 3137 CCGCTC CCGCTC(-3/-3)
4164 GAGCGG
BsrDI 2417 CATTGC GCAATG(2/0)
4337 CATTGC
BstXI 3512 CCAGTGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4118 CCAAAGAGCTGG
Bsu36I 1590 CCTGAGG CC^T[ACGT]AGG
4181 CCTCAGG
CelII 1441 GCTAAGC GC^T[ACGT]AGC
4308 GCTCAGC
DraI 1990 TTTAAA TTT^AAA
2847 TTTAAA
Eco47III 1707 AGCGCT AGC^GCT
3021 AGCGCT
Eco81I 1590 CCTGAGG CC^T[ACGT]AGG
4181 CCTCAGG
EcoNI 2931 CCTCCTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3828 CCTCTCAGAGG
HgaI 2581 GACGC GACGC(5/10)
2869 GACGC
HincII 1258 GTCGAC GT[CT]^[GA]AC
4332 GTCAAC
HindII 1258 GTCGAC GT[CT]^[GA]AC
4332 GTCAAC
KpnI 2442 GGTACC GGTAC^C
2484 GGTACC
KspI 749 CCGCGG CCGC^GG
1193 CCGCGG
MslI 2221 CACATAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3252 CACAGAAGTG
NspI 695 ACATGC [GA]CATG^[CT]
2336 GCATGT
PmaCI 1867 CACGTG CAC^GTG
2526 CACGTG
PmlI 1867 CACGTG CAC^GTG
2526 CACGTG
PvuII 1120 CAGCTG CAG^CTG
3278 CAGCTG
SacII 749 CCGCGG CCGC^GG
1193 CCGCGG
TfiI 621 GATTC G^A[AT]TC
860 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI ApaI AspHI AvaI AvaII
BanI BanII BbsI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I BspMI BsrFI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EcoO109I
EcoRII FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NdeII NgoMIV
NlaIII NlaIV PleI PpuMI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspI TaqI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoII