Restriction map for
pF1KA1070
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# NO CUT ENZYMES ( 119 enzymes) #
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AatII AccII AccIII AclI AcyI
AflIII AgeI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI AspHI AspI AvaI AviII
AvrII BamHI BanII BbeI BciVI
BclI BglI BglII BlnI BlpI
Bpu1102I BsaHI BsaI BsaWI BseAI
BsiEI BsiHKAI BsiWI BsoBI Bsp1407I
BspDI BspEI BspLU11I BsrBI BsrFI
BsrGI BssHII BssSI BstBI BstUI
Bsu36I CelII Cfr10I ClaI DraIII
DrdI EagI EclXI Eco47III Eco52I
Eco81I EcoNI EcoRI EcoT22I FauI
FbaI FseI FspI HaeII HgaI
Hin1I HpaI KasI KspI MfeI
MluI MroI MunI MvnI NaeI
NarI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmeI PshAI PshBI
Psp1406I PstI PvuI SacI SacII
SalI SapI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI Sse8387I
SspBI SspI StuI Tth111I Van91I
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 47 enzymes) #
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Acc65I 1827 GGTACC G^GTACC
AflII 756 CTTAAG C^TTAAG
AhdI 2265 GACCGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 1919 GAAAAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1827 GGTACC G^GTACC
AspEI 2265 GACCGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbrPI 18 CACGTG CAC^GTG
BbsI 451 GAAGAC GAAGAC(2/6)
BbvCI 2140 CCTCAGC CCTCAGC(-5/-2)
BcnI 66 CCGGG CC^[GC]GG
BfrI 756 CTTAAG C^TTAAG
BmyI 77 GTGCCC G[AGT]GC[ACT]^C
BpuAI 451 GAAGAC GAAGAC(2/6)
BsgI 1607 GTGCAG GTGCAG(16/14)
BsmBI 1517 GAGACG CGTCTC(1/5)
BsmI 994 GAATGC GAATGC(1/-1)
Bsp1286I 77 GTGCCC G[AGT]GC[ACT]^C
BspHI 2223 TCATGA T^CATGA
BstAPI 928 GCAAAATATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 301 CCATCTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 2145 GCGC GCG^C
CpoI 2263 CGGACCG CG^G[AT]CCG
DraI 465 TTTAAA TTT^AAA
Eam1105I 2265 GACCGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 2179 GAAGAG CTCTTC(1/4)
HapII 66 CCGG C^CGG
HhaI 2145 GCGC GCG^C
HinP1I 2145 GCGC G^CGC
HindIII 707 AAGCTT A^AGCTT
HpaII 66 CCGG C^CGG
KpnI 1827 GGTACC GGTAC^C
Ksp632I 2179 GAAGAG CTCTTC(1/4)
MspI 66 CCGG C^CGG
NciI 66 CCGGG CC^[GC]GG
NcoI 537 CCATGG C^CATGG
NspI 52 GCATGC [GA]CATG^[CT]
PmaCI 18 CACGTG CAC^GTG
PmlI 18 CACGTG CAC^GTG
PpuMI 510 GGGTCCC [GA]G^G[AT]CC[CT]
RcaI 2223 TCATGA T^CATGA
RsrII 2263 CGGACCG CG^G[AT]CCG
ScaI 639 AGTACT AGT^ACT
SnaBI 1446 TACGTA TAC^GTA
SpeI 721 ACTAGT A^CTAGT
SphI 52 GCATGC GCATG^C
SwaI 464 ATTTAAAT ATTT^AAAT
XmnI 1919 GAAAAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccI 1317 GTATAC GT^[AC][GT]AC
1863 GTATAC
AciI 2267 CCGC CCGC(-3/-1)
1417 GCGG
BcgI 663 CGATCAGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
602 GCAATGTGATCG
BpmI 290 CTCCAG CTGGAG(16/14)
2402 CTGGAG
BsaAI 18 CACGTG [CT]AC^GT[GA]
1446 TACGTA
BsaBI 609 GATCGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2215 GATTTGGATC
BseRI 2109 GAGGAG GAGGAG(10/8)
2298 GAGGAG
BsmAI 1517 GAGAC GTCTC(1/5)
2112 GAGAC
BspMI 803 ACCTGC ACCTGC(4/8)
924 ACCTGC
Bst1107I 1317 GTATAC GTA^TAC
1863 GTATAC
BstYI 775 GGATCT [GA]^GATC[CT]
1419 GGATCT
BstZ17I 1317 GTATAC GTA^TAC
1863 GTATAC
Cac8I 52 GCATGC GC[ACGT]^[ACGT]GC
118 GCTGGC
DraII 222 GGGGCCT [GA]G^G[ACGT]CC[CT]
510 GGGTCCC
DsaI 537 CCATGG C^C[GA][CT]GG
1512 CCACGG
EcoO109I 222 GGGGCCT [GA]G^G[ACGT]CC[CT]
510 GGGTCCC
EcoRV 316 GATATC GAT^ATC
1271 GATATC
EcoT14I 537 CCATGG C^C[AT][AT]GG
1164 CCAAGG
MamI 609 GATCGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2215 GATTTGGATC
MflI 775 GGATCT [GA]^GATC[CT]
1419 GGATCT
NdeI 251 CATATG CA^TATG
431 CATATG
PvuII 903 CAGCTG CAG^CTG
1403 CAGCTG
StyI 537 CCATGG C^C[AT][AT]GG
1164 CCAAGG
TaqI 630 TCGA T^CGA
1050 TCGA
TthHB8I 630 TCGA T^CGA
1050 TCGA
XhoII 775 GGATCT [GA]^GATC[CT]
1419 GGATCT
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# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
AvaII BalI BanI BbvI BfaI
BsaJI BsiYI BslI BsmFI BsrDI
BsrI BssKI BstEII BstF5I BstNI
BstPI Cfr13I DdeI DpnII EaeI
EcoO65I EcoRII Fnu4HI FokI HaeIII
HincII HindII HinfI HphI ItaI
MaeI MaeII MaeIII MboI MboII
MluNI MnlI MscI MseI MslI
MspA1I MvaI MwoI NdeII NlaIII
NlaIV PleI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
XcmI