Restriction map for
pF1KA1002
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# NO CUT ENZYMES ( 96 enzymes) #
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AatII AccI AccII AclI AflII
AflIII AgeI Aor51HI ApaI AscI
Asp700I AviII AvrII BbrPI BbsI
BcgI BciVI BfrI BglI BlnI
BpuAI BsaAI BsaBI BsiEI BsiWI
BsmI BspDI BspHI BspLU11I BsrBI
BsrDI BsrFI BssHII BssSI Bst1107I
BstUI BstZ17I Cfr10I ClaI CpoI
DraI DrdI EagI EclXI Eco47III
Eco52I EcoRI EcoT22I FseI FspI
HgaI HindIII HpaI KspI MamI
MfeI MluI MunI MvnI NaeI
NdeI NgoMIV NotI NruI NsiI
PacI PaeR7I PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI SfiI SgrAI
SmaI SnaBI Sse8387I SspI SwaI
Van91I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 60 enzymes) #
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Acc65I 1530 GGTACC G^GTACC
AccIII 602 TCCGGA T^CCGGA
AcyI 2272 GGCGCC G[GA]^CG[CT]C
AhdI 614 GACCCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2089 GTGCAC G^TGCAC
ApaLI 2089 GTGCAC G^TGCAC
AseI 139 ATTAAT AT^TAAT
AsnI 139 ATTAAT AT^TAAT
Asp718I 1530 GGTACC G^GTACC
AspEI 614 GACCCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 2089 GTGCAC G[AT]GC[AT]^C
AspI 1943 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 662 CCCGAG C^[CT]CG[GA]G
BamHI 2495 GGATCC G^GATCC
BanII 1578 GAGCCC G[GA]GC[CT]^C
BbeI 2272 GGCGCC GGCGC^C
BclI 243 TGATCA T^GATCA
BglII 2208 AGATCT A^GATCT
BsaHI 2272 GGCGCC G[GA]^CG[CT]C
BsaI 1820 GAGACC GGTCTC(1/5)
BseAI 602 TCCGGA T^CCGGA
BseRI 1359 GAGGAG GAGGAG(10/8)
BsgI 1935 CTGCAC GTGCAG(16/14)
BsiHKAI 2089 GTGCAC G[AT]GC[AT]^C
BsmBI 2434 CGTCTC CGTCTC(1/5)
BsoBI 662 CCCGAG C^[CT]CG[GA]G
BspEI 602 TCCGGA T^CCGGA
BspMI 1557 ACCTGC ACCTGC(4/8)
BstBI 2465 TTCGAA TT^CGAA
BstEII 756 GGTCACC G^GT[ACGT]ACC
BstPI 756 GGTCACC G^GT[ACGT]ACC
DraII 1340 AGGGCCT [GA]G^G[ACGT]CC[CT]
Eam1105I 614 GACCCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 2248 CTCTTC CTCTTC(1/4)
EcoNI 1582 CCTTCTGTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 1340 AGGGCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 756 GGTCACC G^GT[ACGT]ACC
EcoRV 416 GATATC GAT^ATC
FbaI 243 TGATCA T^GATCA
Hin1I 2272 GGCGCC G[GA]^CG[CT]C
HincII 2076 GTCAAC GT[CT]^[GA]AC
HindII 2076 GTCAAC GT[CT]^[GA]AC
KasI 2272 GGCGCC G^GCGCC
KpnI 1530 GGTACC GGTAC^C
Ksp632I 2248 CTCTTC CTCTTC(1/4)
MaeII 1227 ACGT A^CGT
MroI 602 TCCGGA T^CCGGA
MseI 140 TTAA T^TAA
NarI 2272 GGCGCC GG^CGCC
NheI 631 GCTAGC G^CTAGC
NspV 2465 TTCGAA TT^CGAA
PflFI 1943 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 139 ATTAAT AT^TAAT
ScaI 1673 AGTACT AGT^ACT
SfoI 2272 GGCGCC GGC^GCC
SfuI 2465 TTCGAA TT^CGAA
SpeI 277 ACTAGT A^CTAGT
SphI 608 GCATGC GCATG^C
Tru9I 140 TTAA T^TAA
Tth111I 1943 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 22 enzymes) #
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AocI 2215 CCTGAGG CC^T[ACGT]AGG
2490 CCTGAGG
BlpI 1347 GCTCAGC GC^T[ACGT]AGC
1561 GCTCAGC
Bpu1102I 1347 GCTCAGC GC^T[ACGT]AGC
1561 GCTCAGC
BsaWI 191 ACCGGA [AT]^CCGG[AT]
602 TCCGGA
Bsp1407I 1826 TGTACA T^GTACA
2378 TGTACA
BsrGI 1826 TGTACA T^GTACA
2378 TGTACA
Bsu36I 2215 CCTGAGG CC^T[ACGT]AGG
2490 CCTGAGG
CelII 1347 GCTCAGC GC^T[ACGT]AGC
1561 GCTCAGC
CfoI 2007 GCGC GCG^C
2273 GCGC
Eco81I 2215 CCTGAGG CC^T[ACGT]AGG
2490 CCTGAGG
HaeII 2006 GGCGCT [GA]GCGC^[CT]
2272 GGCGCC
HhaI 2007 GCGC GCG^C
2273 GCGC
HinP1I 2007 GCGC G^CGC
2273 GCGC
MslI 1857 CACACAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2382 CACCATTGTG
NcoI 1760 CCATGG C^CATGG
2067 CCATGG
NspI 608 GCATGC [GA]CATG^[CT]
1118 ACATGC
PstI 968 CTGCAG CTGCA^G
1647 CTGCAG
SexAI 752 ACCAGGT A^CC[AT]GGT
1698 ACCAGGT
SspBI 1826 TGTACA T^GTACA
2378 TGTACA
StuI 1386 AGGCCT AGG^CCT
1512 AGGCCT
TaqI 1191 TCGA T^CGA
2466 TCGA
TthHB8I 1191 TCGA T^CGA
2466 TCGA
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# 3 OR MORE CUT ENZYMES ( 80 enzymes) #
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AciI AcsI AfaI AluI AlwI
AlwNI ApoI AvaII BalI BanI
BbvCI BbvI BcnI BfaI BmyI
BpmI BsaJI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstAPI
BstF5I BstNI BstXI BstYI Cac8I
Cfr13I DdeI DpnII DraIII DsaI
EaeI EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI MaeI MaeIII MboI
MboII MflI MluNI MnlI MscI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PleI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TfiI TseI
Tsp45I Tsp509I TspRI XcmI XhoII