Restriction map for
pF1KA0985
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# NO CUT ENZYMES ( 97 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AflII AgeI AhdI AscI AseI
AsnI Asp718I AspEI AvrII BamHI
BbeI BcgI BciVI BclI BfrI
BglII BlnI BlpI Bpu1102I BsaBI
BsiEI BsiWI BspDI BspHI BspLU11I
BsrBI BsrDI BssHII Bst1107I BstBI
BstEII BstPI BstUI BstZ17I CelII
ClaI CpoI DraI EagI Eam1105I
EclXI Eco52I EcoO65I EcoRV EcoT22I
FbaI FseI HincII HindII HpaI
KasI KpnI KspI MamI MfeI
MluI MunI MvnI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PinAI PmeI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspI
SwaI XbaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccIII 1057 TCCGGA T^CCGGA
AcyI 230 GACGCC G[GA]^CG[CT]C
AflIII 2069 ACGTGT A^C[GA][CT]GT
AocI 1431 CCTCAGG CC^T[ACGT]AGG
ApaI 630 GGGCCC GGGCC^C
Asp700I 1527 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 902 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 698 TGCGCA TGC^GCA
BalI 1464 TGGCCA TGG^CCA
BbrPI 2068 CACGTG CAC^GTG
BbvCI 865 CCTCAGC CCTCAGC(-5/-2)
BsaAI 2068 CACGTG [CT]AC^GT[GA]
BsaHI 230 GACGCC G[GA]^CG[CT]C
BseAI 1057 TCCGGA T^CCGGA
BsmBI 801 GAGACG CGTCTC(1/5)
BsmI 402 GCATTC GAATGC(1/-1)
Bsp1407I 1676 TGTACA T^GTACA
BspEI 1057 TCCGGA T^CCGGA
BsrFI 53 ACCGGC [GA]^CCGG[CT]
BsrGI 1676 TGTACA T^GTACA
BssSI 1387 CTCGTG CACGAG(-5/-1)
BstXI 105 CCACCCCGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1431 CCTCAGG CC^T[ACGT]AGG
Cfr10I 53 ACCGGC [GA]^CCGG[CT]
DraIII 369 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 1464 TGGCCA [CT]^GGCC[GA]
Eco81I 1431 CCTCAGG CC^T[ACGT]AGG
FauI 1106 CCCGC CCCGC(4/6)
FspI 698 TGCGCA TGC^GCA
Hin1I 230 GACGCC G[GA]^CG[CT]C
HindIII 1333 AAGCTT A^AGCTT
MluNI 1464 TGGCCA TGG^CCA
MroI 1057 TCCGGA T^CCGGA
MscI 1464 TGGCCA TGG^CCA
MslI 106 CACCCCGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NspI 1415 ACATGC [GA]CATG^[CT]
PflFI 902 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1018 CCAGCAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 2068 CACGTG CAC^GTG
PmlI 2068 CACGTG CAC^GTG
SacI 723 GAGCTC GAGCT^C
SspBI 1676 TGTACA T^GTACA
Tth111I 902 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1018 CCAGCAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 371 CCAAGTGCGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 1527 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
Alw44I 1230 GTGCAC G^TGCAC
1723 GTGCAC
AlwNI 647 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
659 CAGCCTCTG
Aor51HI 1988 AGCGCT AGC^GCT
2039 AGCGCT
ApaLI 1230 GTGCAC G^TGCAC
1723 GTGCAC
BanI 1180 GGTGCC G^G[CT][GA]CC
2045 GGCACC
BfaI 247 CTAG C^TAG
731 CTAG
BglI 120 GCCTGACAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
706 GCCTCCGAGGC
BsaWI 1057 TCCGGA [AT]^CCGG[AT]
1785 ACCGGA
DsaI 1262 CCATGG C^C[GA][CT]GG
1736 CCATGG
Eco47III 1988 AGCGCT AGC^GCT
2039 AGCGCT
EcoNI 832 CCTGCCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1605 CCTTTATGAGG
EcoRI 1189 GAATTC G^AATTC
1846 GAATTC
MaeI 247 CTAG C^TAG
731 CTAG
NcoI 1262 CCATGG C^CATGG
1736 CCATGG
PaeR7I 188 CTCGAG C^TCGAG
605 CTCGAG
SapI 142 GAAGAGC GCTCTTC(1/4)
1615 GAAGAGC
StuI 821 AGGCCT AGG^CCT
1695 AGGCCT
XhoI 188 CTCGAG C^TCGAG
605 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 97 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI AvaI AvaII BanII
BbsI BbvI BcnI BmyI BpmI
BpuAI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrI BssKI
BstAPI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DrdI EarI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeII MaeIII
MboI MboII MflI MnlI MseI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII
XmaCI XmaI