Restriction map for
pF1KA0968
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# NO CUT ENZYMES ( 102 enzymes) #
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AatII AccI AccII AclI AcsI
AflIII AgeI AhdI Aor51HI ApaI
ApoI AscI AseI AsnI AspEI
AviII AvrII BamHI BanII BbrPI
BbvCI BcgI BfaI BlnI BlpI
Bpu1102I BsaAI BsaBI BsiEI BsmBI
BsmI BspHI BspLU11I BssHII BssSI
Bst1107I BstAPI BstEII BstPI BstUI
BstZ17I CelII CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoO65I EcoRI EcoT22I FseI
FspI HincII HindII HindIII HpaI
KspI MaeI MaeII MamI MfeI
MluI MunI MvnI NcoI NdeI
NheI NotI NruI NsiI PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshBI Psp1406I PstI PvuI RcaI
RsrII SacI SacII SalI SbfI
ScaI SfcI SfiI SgrAI SnaBI
SpeI Sse8387I SspI StuI SwaI
XbaI XhoI
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# ONE CUT ENZYMES ( 51 enzymes) #
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Acc65I 626 GGTACC G^GTACC
AccIII 940 TCCGGA T^CCGGA
AflII 796 CTTAAG C^TTAAG
AocI 1144 CCTGAGG CC^T[ACGT]AGG
Asp700I 870 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 626 GGTACC G^GTACC
AvaI 308 CCCGGG C^[CT]CG[GA]G
BbsI 1021 GAAGAC GAAGAC(2/6)
BciVI 344 GTATCC GTATCC(6/5)
BclI 740 TGATCA T^GATCA
BfrI 796 CTTAAG C^TTAAG
BglI 1370 GCCGGGACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1405 AGATCT A^GATCT
BpuAI 1021 GAAGAC GAAGAC(2/6)
BsaWI 940 TCCGGA [AT]^CCGG[AT]
BseAI 940 TCCGGA T^CCGGA
BsiWI 566 CGTACG C^GTACG
BsoBI 308 CCCGGG C^[CT]CG[GA]G
Bsp1407I 608 TGTACA T^GTACA
BspDI 1184 ATCGAT AT^CGAT
BspEI 940 TCCGGA T^CCGGA
BspMI 13 ACCTGC ACCTGC(4/8)
BsrBI 819 CCGCTC CCGCTC(-3/-3)
BsrDI 1085 GCAATG GCAATG(2/0)
BsrFI 679 GCCGGC [GA]^CCGG[CT]
BsrGI 608 TGTACA T^GTACA
BstBI 1138 TTCGAA TT^CGAA
Bsu36I 1144 CCTGAGG CC^T[ACGT]AGG
Cfr10I 679 GCCGGC [GA]^CCGG[CT]
ClaI 1184 ATCGAT AT^CGAT
Eco81I 1144 CCTGAGG CC^T[ACGT]AGG
EcoNI 1161 CCTGGTTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 731 CCAAGG C^C[AT][AT]GG
FbaI 740 TGATCA T^GATCA
HgaI 1315 GACGC GACGC(5/10)
KpnI 626 GGTACC GGTAC^C
MroI 940 TCCGGA T^CCGGA
NaeI 679 GCCGGC GCC^GGC
NgoMIV 679 GCCGGC G^CCGGC
NspI 839 GCATGC [GA]CATG^[CT]
NspV 1138 TTCGAA TT^CGAA
PshAI 1353 GACCCGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SfuI 1138 TTCGAA TT^CGAA
SmaI 308 CCCGGG CCC^GGG
SphI 839 GCATGC GCATG^C
SspBI 608 TGTACA T^GTACA
StyI 731 CCAAGG C^C[AT][AT]GG
XcmI 697 CCATCGCCGGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 308 CCCGGG C^CCGGG
XmaI 308 CCCGGG C^CCGGG
XmnI 870 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AcyI 682 GGCGCC G[GA]^CG[CT]C
1413 GGCGCC
Alw44I 394 GTGCAC G^TGCAC
1228 GTGCAC
ApaLI 394 GTGCAC G^TGCAC
1228 GTGCAC
AspI 268 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
712 GACACTGTC
BbeI 682 GGCGCC GGCGC^C
1413 GGCGCC
BpmI 169 CTGGAG CTGGAG(16/14)
358 CTGGAG
BsaHI 682 GGCGCC G[GA]^CG[CT]C
1413 GGCGCC
BseRI 241 GAGGAG GAGGAG(10/8)
1348 GAGGAG
BsgI 15 CTGCAC GTGCAG(16/14)
370 CTGCAC
CfoI 683 GCGC GCG^C
1414 GCGC
DsaI 827 CCGTGG C^C[GA][CT]GG
857 CCGTGG
EcoRV 298 GATATC GAT^ATC
533 GATATC
FauI 20 CCCGC CCCGC(4/6)
182 CCCGC
HaeII 682 GGCGCC [GA]GCGC^[CT]
1413 GGCGCC
HhaI 683 GCGC GCG^C
1414 GCGC
Hin1I 682 GGCGCC G[GA]^CG[CT]C
1413 GGCGCC
HinP1I 683 GCGC G^CGC
1414 GCGC
KasI 682 GGCGCC G^GCGCC
1413 GGCGCC
MseI 761 TTAA T^TAA
797 TTAA
MspA1I 782 CAGCTG C[AC]G^C[GT]G
1066 CAGCTG
NarI 682 GGCGCC GG^CGCC
1413 GGCGCC
PflFI 268 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
712 GACACTGTC
PflMI 378 CCACCAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1257 CCACCTGATGG
PpuMI 401 GGGACCT [GA]G^G[AT]CC[CT]
560 AGGACCC
PvuII 782 CAGCTG CAG^CTG
1066 CAGCTG
SapI 966 GAAGAGC GCTCTTC(1/4)
42 GCTCTTC
SexAI 269 ACCTGGT A^CC[AT]GGT
1160 ACCTGGT
SfoI 682 GGCGCC GGC^GCC
1413 GGCGCC
SmlI 439 CTCAAG C^T[CT][GA]AG
796 CTTAAG
Tru9I 761 TTAA T^TAA
797 TTAA
Tsp509I 53 AATT ^AATT
1047 AATT
Tth111I 268 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
712 GACACTGTC
Van91I 378 CCACCAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1257 CCACCTGATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 72 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AspHI AvaII BalI BanI BbvI
BcnI BmyI BsaI BsaJI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI BstXI
BstYI Cac8I Cfr13I DdeI DpnII
DraII EaeI EarI EcoO109I EcoRII
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI Ksp632I MaeIII
MboI MboII MflI MluNI MnlI
MscI MslI MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PleI
RsaI Sau3AI Sau96I ScrFI SfaNI
TaqI TfiI TseI Tsp45I TspRI
TthHB8I XhoII