Restriction map for
pF1KA0937
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# NO CUT ENZYMES ( 98 enzymes) #
##################################
AatII Acc65I AclI AcsI AflII
AflIII AgeI Alw44I Aor51HI ApaLI
ApoI AscI AseI AsnI Asp718I
AviII AvrII BbrPI BbvCI BcgI
BciVI BclI BfaI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaBI
BsaI BsiWI BspDI BspLU11I BsrBI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I CelII ClaI CpoI
DraI DraIII DrdI Eco47III EcoNI
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HindIII HpaI KpnI
KspI MaeI MamI MfeI MluI
MunI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RsrII SacI SacII SalI
SapI SbfI ScaI SfiI SfuI
SmlI SpeI Sse8387I SspI StuI
SwaI XbaI XhoI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccI 1327 GTCTAC GT^[AC][GT]AC
AhdI 175 GACCTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 175 GACCTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 43 GAGCAC G[AT]GC[AT]^C
AspI 1705 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 1007 GGATCC G^GATCC
BbeI 464 GGCGCC GGCGC^C
BsaAI 397 TACGTA [CT]AC^GT[GA]
BseRI 249 CTCCTC GAGGAG(10/8)
BsiHKAI 43 GAGCAC G[AT]GC[AT]^C
BsmBI 154 CGTCTC CGTCTC(1/5)
BsmI 1529 GCATTC GAATGC(1/-1)
Bsp1407I 1355 TGTACA T^GTACA
BspHI 1013 TCATGA T^CATGA
BsrDI 903 CATTGC GCAATG(2/0)
BsrGI 1355 TGTACA T^GTACA
BssSI 1642 CTCGTG CACGAG(-5/-1)
BstYI 1007 GGATCC [GA]^GATC[CT]
Eam1105I 175 GACCTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1560 GAAGAG CTCTTC(1/4)
HincII 979 GTCAAC GT[CT]^[GA]AC
HindII 979 GTCAAC GT[CT]^[GA]AC
KasI 464 GGCGCC G^GCGCC
Ksp632I 1560 GAAGAG CTCTTC(1/4)
MflI 1007 GGATCC [GA]^GATC[CT]
MseI 599 TTAA T^TAA
NarI 464 GGCGCC GG^CGCC
PflFI 1705 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 697 CCAGGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 1699 GAGTC GAGTC(4/5)
PshAI 148 GACAGCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 637 CAGCTG CAG^CTG
RcaI 1013 TCATGA T^CATGA
SexAI 1295 ACCTGGT A^CC[AT]GGT
SfoI 464 GGCGCC GGC^GCC
SgrAI 460 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 914 CCCGGG CCC^GGG
SnaBI 397 TACGTA TAC^GTA
SspBI 1355 TGTACA T^GTACA
Tru9I 599 TTAA T^TAA
Tth111I 1705 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 697 CCAGGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1467 CCACTCCTTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 1007 GGATCC [GA]^GATC[CT]
XmaCI 914 CCCGGG C^CCGGG
XmaI 914 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AlwNI 698 CAGGCTCTG CAG[ACGT][ACGT][ACGT]^CTG
892 CAGCGACTG
AocI 505 CCTAAGG CC^T[ACGT]AGG
1256 CCTCAGG
ApaI 628 GGGCCC GGGCC^C
1269 GGGCCC
Asp700I 189 GAACCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1560 GAAGAGTTTC
AvaI 914 CCCGGG C^[CT]CG[GA]G
1574 CCCGAG
BbsI 750 GAAGAC GAAGAC(2/6)
1419 GAAGAC
BpuAI 750 GAAGAC GAAGAC(2/6)
1419 GAAGAC
BsgI 1224 CTGCAC GTGCAG(16/14)
1019 GTGCAG
BsiEI 14 CGGCCG CG[GA][CT]^CG
48 CGGCCG
BsoBI 914 CCCGGG C^[CT]CG[GA]G
1574 CCCGAG
BsrFI 106 GCCGGC [GA]^CCGG[CT]
461 GCCGGC
Bsu36I 505 CCTAAGG CC^T[ACGT]AGG
1256 CCTCAGG
Cfr10I 106 GCCGGC [GA]^CCGG[CT]
461 GCCGGC
EagI 14 CGGCCG C^GGCCG
48 CGGCCG
EclXI 14 CGGCCG C^GGCCG
48 CGGCCG
Eco52I 14 CGGCCG C^GGCCG
48 CGGCCG
Eco81I 505 CCTAAGG CC^T[ACGT]AGG
1256 CCTCAGG
MaeII 398 ACGT A^CGT
1325 ACGT
NaeI 106 GCCGGC GCC^GGC
461 GCCGGC
NgoMIV 106 GCCGGC G^CCGGC
461 GCCGGC
PstI 178 CTGCAG CTGCA^G
889 CTGCAG
SphI 342 GCATGC GCATG^C
791 GCATGC
Tsp45I 490 GTCAC ^GT[GC]AC
646 GTGAC
Tsp509I 402 AATT ^AATT
1783 AATT
XmnI 189 GAACCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1560 GAAGAGTTTC
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# 3 OR MORE CUT ENZYMES ( 89 enzymes) #
#########################################
AccII AccIII AciI AcyI AfaI
AluI AlwI AvaII BalI BanI
BanII BbvI BcnI BmyI BpmI
BsaHI BsaJI BsaWI BseAI BsiYI
BslI BsmAI BsmFI Bsp1286I BspEI
BspMI BsrI BssKI BstF5I BstNI
BstUI BstXI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI MaeIII MboI MboII
MluNI MnlI MroI MscI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PpuMI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TaqI
TfiI TseI TspRI TthHB8I