Restriction map for
pF1KA0933
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# NO CUT ENZYMES ( 53 enzymes) #
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AatII Acc65I AccIII AclI AgeI
AscI AseI AsnI Asp718I BbrPI
BseAI BsiWI BsmBI BspDI BspEI
BssHII Bst1107I BstBI BstEII BstPI
BstZ17I ClaI CpoI EcoO65I EcoRV
FseI KpnI KspI MfeI MluI
MroI MunI NdeI NheI NotI
NruI NspV PacI PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RsrII SacII SfuI SgrAI SnaBI
SpeI SspI SwaI
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# ONE CUT ENZYMES ( 27 enzymes) #
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AflII 1557 CTTAAG C^TTAAG
AvrII 4797 CCTAGG C^CTAGG
BbeI 3151 GGCGCC GGCGC^C
BcgI 1968 CGAGGAGCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 1557 CTTAAG C^TTAAG
BlnI 4797 CCTAGG C^CTAGG
BsaBI 2611 GATTTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1791 ACCGGA [AT]^CCGG[AT]
BspLU11I 866 ACATGT A^CATGT
BsrBI 2988 GAGCGG CCGCTC(-3/-3)
DraIII 5053 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
HindIII 3351 AAGCTT A^AGCTT
HpaI 4729 GTTAAC GTT^AAC
KasI 3151 GGCGCC G^GCGCC
MamI 2611 GATTTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 5374 GCCGGC GCC^GGC
NarI 3151 GGCGCC GG^CGCC
NgoMIV 5374 GCCGGC G^CCGGC
PshAI 3744 GACCCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SalI 5005 GTCGAC G^TCGAC
ScaI 392 AGTACT AGT^ACT
SexAI 5054 ACCTGGT A^CC[AT]GGT
SfiI 3504 GGCCGTCGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 3151 GGCGCC GGC^GCC
SmaI 4253 CCCGGG CCC^GGG
XmaCI 4253 CCCGGG C^CCGGG
XmaI 4253 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 28 enzymes) #
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AccI 2293 GTCTAC GT^[AC][GT]AC
5005 GTCGAC
AcyI 3151 GGCGCC G[GA]^CG[CT]C
4023 GGCGTC
Alw44I 3281 GTGCAC G^TGCAC
5018 GTGCAC
ApaLI 3281 GTGCAC G^TGCAC
5018 GTGCAC
AspI 1941 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
4498 GACTTGGTC
AviII 824 TGCGCA TGC^GCA
2965 TGCGCA
BamHI 3 GGATCC G^GATCC
4813 GGATCC
BanI 2764 GGTGCC G^G[CT][GA]CC
3151 GGCGCC
BbvCI 1540 GCTGAGG CCTCAGC(-5/-2)
5461 GCTGAGG
BciVI 3648 GGATAC GTATCC(6/5)
5799 GGATAC
BclI 3902 TGATCA T^GATCA
5973 TGATCA
BsaAI 3121 TACGTG [CT]AC^GT[GA]
4306 TACGTG
BsaHI 3151 GGCGCC G[GA]^CG[CT]C
4023 GGCGTC
BsiEI 636 CGGCCG CG[GA][CT]^CG
2909 CGGCCG
BssSI 2105 CACGAG CACGAG(-5/-1)
4035 CACGAG
DraI 271 TTTAAA TTT^AAA
4831 TTTAAA
DrdI 3874 GACGTGCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
6658 GACTTTCCTGTC
EagI 636 CGGCCG C^GGCCG
2909 CGGCCG
EclXI 636 CGGCCG C^GGCCG
2909 CGGCCG
Eco52I 636 CGGCCG C^GGCCG
2909 CGGCCG
FbaI 3902 TGATCA T^GATCA
5973 TGATCA
FspI 824 TGCGCA TGC^GCA
2965 TGCGCA
Hin1I 3151 GGCGCC G[GA]^CG[CT]C
4023 GGCGTC
PflFI 1941 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
4498 GACTTGGTC
PflMI 2427 CCAGAACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2472 CCAGTCCCTGG
Tth111I 1941 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
4498 GACTTGGTC
Van91I 2427 CCAGAACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2472 CCAGTCCCTGG
XcmI 3368 CCAAGTTAAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3635 CCAGGACTAGCATGG
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# 3 OR MORE CUT ENZYMES ( 150 enzymes) #
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AccII AciI AcsI AfaI AflIII
AhdI AluI AlwI AlwNI AocI
Aor51HI ApaI ApoI Asp700I AspEI
AspHI AvaI AvaII BalI BanII
BbsI BbvI BcnI BfaI BglI
BglII BlpI BmyI BpmI Bpu1102I
BpuAI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I Bsp1407I BspHI
BspMI BsrDI BsrFI BsrGI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstXI BstYI Bsu36I Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI Eam1105I EarI
Eco47III Eco81I EcoNI EcoO109I EcoRI
EcoRII EcoT14I EcoT22I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
NsiI NspI PaeR7I PleI PpuMI
PstI PvuII RcaI RsaI SacI
SapI Sau3AI Sau96I SbfI ScrFI
SfaNI SfcI SmlI SphI Sse8387I
SspBI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XbaI XhoI XhoII XmnI