Restriction map for
pF1KA0900
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# NO CUT ENZYMES ( 111 enzymes) #
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AatII Acc65I AccI AccII AclI
AflII AflIII AgeI AhdI Alw44I
Aor51HI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
AvrII BbrPI BcgI BciVI BclI
BfrI BglI BglII BlnI BsaAI
BsaBI BsiEI BsiWI BsmBI Bsp1407I
BspDI BspHI BspLU11I BspMI BsrBI
BsrGI BssHII BssSI Bst1107I BstEII
BstPI BstUI BstXI BstZ17I ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoO65I
FbaI FseI FspI HincII HindII
HpaI KpnI KspI MamI MfeI
MluI MunI MvnI NaeI NgoMIV
NheI NotI NruI PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsrII SacII SalI SapI
SbfI SexAI SfiI SgrAI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI Tth111I XbaI XhoI
XmnI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AccIII 1021 TCCGGA T^CCGGA
AlwNI 1268 CAGTAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 928 CCTCAGG CC^T[ACGT]AGG
ApaI 1194 GGGCCC GGGCC^C
AspHI 47 GAGCTC G[AT]GC[AT]^C
BalI 1421 TGGCCA TGG^CCA
BamHI 694 GGATCC G^GATCC
BbeI 602 GGCGCC GGCGC^C
BfaI 713 CTAG C^TAG
BlpI 120 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 120 GCTGAGC GC^T[ACGT]AGC
BsaI 267 GGTCTC GGTCTC(1/5)
BsaWI 1021 TCCGGA [AT]^CCGG[AT]
BseAI 1021 TCCGGA T^CCGGA
BsiHKAI 47 GAGCTC G[AT]GC[AT]^C
BsmI 347 GCATTC GAATGC(1/-1)
BspEI 1021 TCCGGA T^CCGGA
BsrDI 1443 CATTGC GCAATG(2/0)
BsrFI 857 ACCGGC [GA]^CCGG[CT]
BstAPI 1063 GCACAAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1343 TTCGAA TT^CGAA
BstYI 694 GGATCC [GA]^GATC[CT]
Bsu36I 928 CCTCAGG CC^T[ACGT]AGG
CelII 120 GCTGAGC GC^T[ACGT]AGC
Cfr10I 857 ACCGGC [GA]^CCGG[CT]
EaeI 1421 TGGCCA [CT]^GGCC[GA]
Eco81I 928 CCTCAGG CC^T[ACGT]AGG
EcoNI 872 CCTCGGCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1352 GAATTC G^AATTC
EcoRV 280 GATATC GAT^ATC
EcoT14I 675 CCATGG C^C[AT][AT]GG
HaeII 602 GGCGCC [GA]GCGC^[CT]
HindIII 296 AAGCTT A^AGCTT
KasI 602 GGCGCC G^GCGCC
MaeI 713 CTAG C^TAG
MflI 694 GGATCC [GA]^GATC[CT]
MluNI 1421 TGGCCA TGG^CCA
MroI 1021 TCCGGA T^CCGGA
MscI 1421 TGGCCA TGG^CCA
MseI 167 TTAA T^TAA
MslI 1443 CATTGCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 602 GGCGCC GG^CGCC
NcoI 675 CCATGG C^CATGG
NdeI 626 CATATG CA^TATG
NspV 1343 TTCGAA TT^CGAA
PpuMI 943 GGGTCCC [GA]G^G[AT]CC[CT]
SacI 47 GAGCTC GAGCT^C
ScaI 94 AGTACT AGT^ACT
SfoI 602 GGCGCC GGC^GCC
SfuI 1343 TTCGAA TT^CGAA
StuI 950 AGGCCT AGG^CCT
StyI 675 CCATGG C^C[AT][AT]GG
Tru9I 167 TTAA T^TAA
XcmI 1160 CCACCGGGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 694 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 23 enzymes) #
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AcsI 198 AAATTC [GA]^AATT[CT]
1352 GAATTC
AcyI 530 GGCGTC G[GA]^CG[CT]C
602 GGCGCC
ApoI 198 AAATTC [GA]^AATT[CT]
1352 GAATTC
AvaI 1191 CCCGGG C^[CT]CG[GA]G
1228 CCCGGG
BanI 559 GGCACC G^G[CT][GA]CC
602 GGCGCC
BpmI 968 CTCCAG CTGGAG(16/14)
1087 CTCCAG
BsaHI 530 GGCGTC G[GA]^CG[CT]C
602 GGCGCC
BsmAI 268 GTCTC GTCTC(1/5)
660 GTCTC
BsoBI 1191 CCCGGG C^[CT]CG[GA]G
1228 CCCGGG
DsaI 675 CCATGG C^C[GA][CT]GG
1448 CCGTGG
EcoT22I 722 ATGCAT ATGCA^T
1255 ATGCAT
HgaI 531 GCGTC GACGC(5/10)
682 GCGTC
Hin1I 530 GGCGTC G[GA]^CG[CT]C
602 GGCGCC
NsiI 722 ATGCAT ATGCA^T
1255 ATGCAT
NspI 138 ACATGC [GA]CATG^[CT]
720 ACATGC
PflMI 324 CCAGCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1252 CCAATGCATGG
PleI 1462 GACTC GAGTC(4/5)
1474 GACTC
SfcI 274 CTACAG C^T[GA][CT]AG
496 CTACAG
SmaI 1191 CCCGGG CCC^GGG
1228 CCCGGG
TfiI 250 GATTC G^A[AT]TC
1053 GATTC
Van91I 324 CCAGCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1252 CCAATGCATGG
XmaCI 1191 CCCGGG C^CCGGG
1228 CCCGGG
XmaI 1191 CCCGGG C^CCGGG
1228 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BanII BbsI BbvCI BbvI BcnI
BmyI BpuAI BsaJI BseRI BsgI
BsiYI BslI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI Cac8I CfoI
Cfr13I DdeI DpnII DraII EarI
EcoO109I EcoRII FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeII
MaeIII MboI MboII MnlI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI TaqI TseI
Tsp45I Tsp509I TspRI TthHB8I