Restriction map for
pF1KA0879
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# NO CUT ENZYMES ( 135 enzymes) #
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AatII Acc65I AccI AccIII AciI
AclI AcyI AflII AflIII AgeI
Alw44I AocI Aor51HI ApaI ApaLI
AscI Asp718I AspHI AvaI AviII
AvrII BanI BbeI BbrPI BbvCI
BcgI BcnI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaHI
BsaI BsaWI BseAI BsgI BsiEI
BsiHKAI BsiWI BsmAI BsmBI BsmI
BsoBI Bsp1407I BspEI BspHI BspLU11I
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstZ17I Bsu36I
Cac8I CelII CfoI Cfr10I CpoI
DraI DraIII DrdI EagI EclXI
Eco47III Eco52I Eco81I EcoNI EcoRV
FauI FseI FspI HaeII HapII
HgaI HhaI Hin1I HinP1I HindIII
HpaII KasI KpnI KspI MaeII
MluI MroI MspA1I MspI NaeI
NarI NciI NgoMIV NheI NotI
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshAI Psp1406I PstI PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SgrAI SmaI SnaBI
SphI Sse8387I SspBI StuI SwaI
XbaI XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AccII 1224 CGCG CG^CG
AhdI 1161 GACCTTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 800 CAGTTGCTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 1204 ATTAAT AT^TAAT
AsnI 1204 ATTAAT AT^TAAT
Asp700I 889 GAAGACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1161 GACCTTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 646 GACTTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 573 TGGCCA TGG^CCA
BamHI 324 GGATCC G^GATCC
BanII 795 GAGCCC G[GA]GC[CT]^C
BbsI 889 GAAGAC GAAGAC(2/6)
BciVI 91 GTATCC GTATCC(6/5)
BmyI 795 GAGCCC G[AGT]GC[ACT]^C
BpmI 145 CTCCAG CTGGAG(16/14)
BpuAI 889 GAAGAC GAAGAC(2/6)
BseRI 465 CTCCTC GAGGAG(10/8)
BsmFI 1159 GGGAC GGGAC(10/14)
Bsp1286I 795 GAGCCC G[AGT]GC[ACT]^C
BspDI 763 ATCGAT AT^CGAT
BspMI 1059 ACCTGC ACCTGC(4/8)
BstBI 519 TTCGAA TT^CGAA
BstUI 1224 CGCG CG^CG
BstXI 938 CCATTATTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 324 GGATCC [GA]^GATC[CT]
ClaI 763 ATCGAT AT^CGAT
DraII 1159 GGGACCT [GA]G^G[ACGT]CC[CT]
EaeI 573 TGGCCA [CT]^GGCC[GA]
Eam1105I 1161 GACCTTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 1159 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoRI 929 GAATTC G^AATTC
EcoT22I 1033 ATGCAT ATGCA^T
HpaI 1251 GTTAAC GTT^AAC
MfeI 968 CAATTG C^AATTG
MflI 324 GGATCC [GA]^GATC[CT]
MluNI 573 TGGCCA TGG^CCA
MscI 573 TGGCCA TGG^CCA
MunI 968 CAATTG C^AATTG
MvnI 1224 CGCG CG^CG
MwoI 1026 GCCTAGTATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeI 868 CATATG CA^TATG
NruI 1223 TCGCGA TCG^CGA
NsiI 1033 ATGCAT ATGCA^T
NspV 519 TTCGAA TT^CGAA
PflFI 646 GACTTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1125 CCACATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1159 GGGACCT [GA]G^G[AT]CC[CT]
PshBI 1204 ATTAAT AT^TAAT
SfuI 519 TTCGAA TT^CGAA
SmlI 661 CTCAAG C^T[CT][GA]AG
SpeI 87 ACTAGT A^CTAGT
SspI 496 AATATT AAT^ATT
Tth111I 646 GACTTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1125 CCACATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 324 GGATCC [GA]^GATC[CT]
XmnI 889 GAAGACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
BclI 695 TGATCA T^GATCA
708 TGATCA
BstEII 360 GGTGACC G^GT[ACGT]ACC
1000 GGTGACC
BstPI 360 GGTGACC G^GT[ACGT]ACC
1000 GGTGACC
EarI 57 CTCTTC CTCTTC(1/4)
441 CTCTTC
EcoO65I 360 GGTGACC G^GT[ACGT]ACC
1000 GGTGACC
EcoT14I 255 CCATGG C^C[AT][AT]GG
1005 CCATGG
FbaI 695 TGATCA T^GATCA
708 TGATCA
HaeIII 407 GGCC GG^CC
574 GGCC
HincII 1189 GTTGAC GT[CT]^[GA]AC
1251 GTTAAC
HindII 1189 GTTGAC GT[CT]^[GA]AC
1251 GTTAAC
Ksp632I 57 CTCTTC CTCTTC(1/4)
441 CTCTTC
MslI 868 CATATGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1006 CATGGTTATG
NcoI 255 CCATGG C^CATGG
1005 CCATGG
NlaIV 324 GGATCC GG[ACGT]^[ACGT]CC
1159 GGGACC
NspI 180 GCATGT [GA]CATG^[CT]
1264 ACATGC
SfcI 222 CTACAG C^T[GA][CT]AG
858 CTGTAG
StyI 255 CCATGG C^C[AT][AT]GG
1005 CCATGG
TfiI 754 GATTC G^A[AT]TC
982 GAATC
#########################################
# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
AvaII BbvI BfaI BsaBI BsaJI
BsiYI BslI BsrI BssKI BstF5I
BstNI Cfr13I DdeI DpnII DsaI
EcoRII Fnu4HI FokI HinfI HphI
ItaI MaeI MaeIII MamI MboI
MboII MnlI MseI MvaI NdeII
NlaIII PleI RsaI Sau3AI Sau96I
ScrFI SfaNI TaqI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I