Restriction map for
pF1KA0878
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# NO CUT ENZYMES ( 124 enzymes) #
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AatII AccI AccIII AflII AflIII
AgeI AhdI Alw44I AlwNI Aor51HI
ApaI ApaLI AscI Asp700I AspEI
AspHI AspI AvaI AviII AvrII
BalI BanII BbeI BbrPI BbvCI
BcgI BciVI BfrI BglII BlnI
BlpI BmyI BpmI Bpu1102I BsaAI
BsaBI BseAI BsgI BsiHKAI BsiWI
BsmBI BsmI BsoBI Bsp1286I BspDI
BspEI BspHI BspLU11I BsrBI BssHII
BssSI Bst1107I BstEII BstPI BstZ17I
CelII ClaI CpoI DraII DsaI
Eam1105I Eco47III EcoNI EcoO109I EcoO65I
EcoRI EcoT14I FauI FseI FspI
HindIII HpaI KasI KspI MamI
MfeI MluI MluNI MroI MscI
MunI NaeI NarI NcoI NdeI
NgoMIV NotI NruI PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SgrAI
SmaI SnaBI SpeI Sse8387I SspI
StuI StyI SwaI Tth111I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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Acc65I 400 GGTACC G^GTACC
AclI 301 AACGTT AA^CGTT
AcyI 997 GACGCC G[GA]^CG[CT]C
AocI 1170 CCTAAGG CC^T[ACGT]AGG
AseI 1162 ATTAAT AT^TAAT
AsnI 1162 ATTAAT AT^TAAT
Asp718I 400 GGTACC G^GTACC
AvaII 207 GGTCC G^G[AT]CC
BamHI 482 GGATCC G^GATCC
BanI 400 GGTACC G^G[CT][GA]CC
BclI 290 TGATCA T^GATCA
BsaHI 997 GACGCC G[GA]^CG[CT]C
BsaI 102 GGTCTC GGTCTC(1/5)
BsaWI 156 TCCGGT [AT]^CCGG[AT]
BseRI 563 GAGGAG GAGGAG(10/8)
BspMI 1604 ACCTGC ACCTGC(4/8)
BsrDI 1665 CATTGC GCAATG(2/0)
BsrFI 56 ACCGGC [GA]^CCGG[CT]
BstAPI 1554 GCAGAGCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1270 TTCGAA TT^CGAA
BstXI 1307 CCATCCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1170 CCTAAGG CC^T[ACGT]AGG
Cfr10I 56 ACCGGC [GA]^CCGG[CT]
DraIII 10 CACATCGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 58 CGGCCG C^GGCCG
EclXI 58 CGGCCG C^GGCCG
Eco52I 58 CGGCCG C^GGCCG
Eco81I 1170 CCTAAGG CC^T[ACGT]AGG
EcoRV 1078 GATATC GAT^ATC
EcoT22I 1137 ATGCAT ATGCA^T
FbaI 290 TGATCA T^GATCA
Hin1I 997 GACGCC G[GA]^CG[CT]C
HincII 1600 GTTGAC GT[CT]^[GA]AC
HindII 1600 GTTGAC GT[CT]^[GA]AC
KpnI 400 GGTACC GGTAC^C
NheI 949 GCTAGC G^CTAGC
NsiI 1137 ATGCAT ATGCA^T
NspV 1270 TTCGAA TT^CGAA
PshBI 1162 ATTAAT AT^TAAT
Psp1406I 301 AACGTT AA^CGTT
SfuI 1270 TTCGAA TT^CGAA
SmlI 1228 CTCAAG C^T[CT][GA]AG
SphI 1559 GCATGC GCATG^C
Tsp45I 711 GTCAC ^GT[GC]AC
XcmI 5 CCATCCACATCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 25 enzymes) #
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BbsI 603 GAAGAC GAAGAC(2/6)
1267 GTCTTC
BcnI 107 CCGGG CC^[GC]GG
1808 CCCGG
BfaI 502 CTAG C^TAG
950 CTAG
BpuAI 603 GAAGAC GAAGAC(2/6)
1267 GTCTTC
BsiEI 58 CGGCCG CG[GA][CT]^CG
146 CGGTCG
Bsp1407I 427 TGTACA T^GTACA
1433 TGTACA
BsrGI 427 TGTACA T^GTACA
1433 TGTACA
DraI 786 TTTAAA TTT^AAA
1143 TTTAAA
DrdI 214 GACTGTCCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1407 GACTTTCTTGTC
HaeII 18 GGCGCT [GA]GCGC^[CT]
274 GGCGCT
HphI 164 TCACC GGTGA(8/7)
1626 TCACC
MaeI 502 CTAG C^TAG
950 CTAG
MaeII 302 ACGT A^CGT
764 ACGT
MaeIII 420 GTTAC ^GT[ACGT]AC
711 GTCAC
MspA1I 1454 CAGCTG C[AC]G^C[GT]G
1549 CAGCTG
NciI 107 CCGGG CC^[GC]GG
1808 CCCGG
NlaIV 400 GGTACC GG[ACGT]^[ACGT]CC
482 GGATCC
NspI 430 ACATGC [GA]CATG^[CT]
1559 GCATGC
PflMI 9 CCACATCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
753 CCAGTGTGTGG
PstI 1516 CTGCAG CTGCA^G
1552 CTGCAG
PvuII 1454 CAGCTG CAG^CTG
1549 CAGCTG
SfaNI 1251 GATGC GCATC(5/9)
1579 GCATC
SfcI 1516 CTGCAG C^T[GA][CT]AG
1552 CTGCAG
SspBI 427 TGTACA T^GTACA
1433 TGTACA
Van91I 9 CCACATCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
753 CCAGTGTGTGG
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# 3 OR MORE CUT ENZYMES ( 64 enzymes) #
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AccII AciI AcsI AfaI AluI
AlwI ApoI BbvI BglI BsaJI
BsiYI BslI BsmAI BsmFI BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
EaeI EarI EcoRII Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII ItaI Ksp632I MboI
MboII MflI MnlI MseI MslI
MspI MvaI MvnI MwoI NdeII
NlaIII PleI RsaI Sau3AI Sau96I
ScrFI TaqI TfiI Tru9I TseI
Tsp509I TspRI TthHB8I XhoII