Restriction map for
pF1KA0834
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# NO CUT ENZYMES ( 138 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI AhdI Aor51HI
ApaI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AviII
AvrII BalI BamHI BanI BbeI
BbrPI BbvCI BcgI BciVI BfrI
BglII BlnI BsaAI BsaBI BsaHI
BsaWI BseAI BsgI BsiEI BsiWI
BsoBI BspDI BspEI BsrBI BsrFI
BssHII BstBI BstUI CfoI Cfr10I
ClaI CpoI DraIII DrdI EaeI
EagI Eam1105I EarI EclXI Eco47III
Eco52I EcoNI EcoRI EcoRV EcoT22I
FseI FspI HaeII HhaI Hin1I
HinP1I HincII HindII HpaI KasI
KpnI Ksp632I KspI MaeII MamI
MfeI MluI MluNI MroI MscI
MspA1I MunI MvnI NaeI NarI
NcoI NgoMIV NheI NlaIV NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI PvuII
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I StuI SwaI TaqI TfiI
Tth111I TthHB8I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AcsI 328 AAATTT [GA]^AATT[CT]
Alw44I 583 GTGCAC G^TGCAC
AocI 142 CCTGAGG CC^T[ACGT]AGG
ApaLI 583 GTGCAC G^TGCAC
ApoI 328 AAATTT [GA]^AATT[CT]
AvaII 1125 GGACC G^G[AT]CC
BanII 34 GAGCCC G[GA]GC[CT]^C
BbsI 1242 GTCTTC GAAGAC(2/6)
BcnI 37 CCCGG CC^[GC]GG
BglI 1212 GCCGCCACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1101 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1101 GCTCAGC GC^T[ACGT]AGC
BpuAI 1242 GTCTTC GAAGAC(2/6)
BsaI 710 GAGACC GGTCTC(1/5)
BseRI 261 CTCCTC GAGGAG(10/8)
BsmBI 556 GAGACG CGTCTC(1/5)
BsmI 225 GCATTC GAATGC(1/-1)
Bsp1407I 1064 TGTACA T^GTACA
BspHI 534 TCATGA T^CATGA
BsrDI 20 GCAATG GCAATG(2/0)
BsrGI 1064 TGTACA T^GTACA
BsrI 268 ACTGG ACTGG(1/-1)
BssSI 1192 CACGAG CACGAG(-5/-1)
Bst1107I 391 GTATAC GTA^TAC
BstAPI 1174 GCACAGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 822 GGTTACC G^GT[ACGT]ACC
BstPI 822 GGTTACC G^GT[ACGT]ACC
BstYI 376 GGATCT [GA]^GATC[CT]
BstZ17I 391 GTATAC GTA^TAC
Bsu36I 142 CCTGAGG CC^T[ACGT]AGG
CelII 1101 GCTCAGC GC^T[ACGT]AGC
DraI 498 TTTAAA TTT^AAA
DraII 1124 AGGACCT [GA]G^G[ACGT]CC[CT]
DsaI 1216 CCACGG C^C[GA][CT]GG
Eco81I 142 CCTGAGG CC^T[ACGT]AGG
EcoO109I 1124 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoO65I 822 GGTTACC G^GT[ACGT]ACC
FauI 1299 GCGGG CCCGC(4/6)
HapII 38 CCGG C^CGG
HgaI 558 GACGC GACGC(5/10)
HindIII 488 AAGCTT A^AGCTT
HpaII 38 CCGG C^CGG
MflI 376 GGATCT [GA]^GATC[CT]
MspI 38 CCGG C^CGG
NciI 37 CCCGG CC^[GC]GG
NdeI 347 CATATG CA^TATG
PpuMI 1124 AGGACCT [GA]G^G[AT]CC[CT]
RcaI 534 TCATGA T^CATGA
SmlI 1185 CTTGAG C^T[CT][GA]AG
SphI 1295 GCATGC GCATG^C
SspBI 1064 TGTACA T^GTACA
XhoII 376 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AccI 87 GTCTAC GT^[AC][GT]AC
391 GTATAC
AflIII 887 ACATGT A^C[GA][CT]GT
1238 ACATGT
AlwNI 661 CAGTTGCTG CAG[ACGT][ACGT][ACGT]^CTG
1280 CAGAAGCTG
AspHI 177 GAGCAC G[AT]GC[AT]^C
583 GTGCAC
BsiHKAI 177 GAGCAC G[AT]GC[AT]^C
583 GTGCAC
BsmAI 556 GAGAC GTCTC(1/5)
710 GAGAC
BspLU11I 887 ACATGT A^CATGT
1238 ACATGT
BspMI 877 ACCTGC ACCTGC(4/8)
1208 ACCTGC
BstXI 812 CCAACGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1216 CCACGGCTATGG
Cfr13I 1125 GGACC G^G[ACGT]CC
1301 GGGCC
EcoT14I 551 CCAAGG C^C[AT][AT]GG
924 CCAAGG
MwoI 1174 GCACAGGCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
1212 GCCGCCACGGC
PflMI 102 CCAGGGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1279 CCAGAAGCTGG
PstI 29 CTGCAG CTGCA^G
448 CTGCAG
Sau96I 1125 GGACC G^G[ACGT]CC
1301 GGGCC
SfaNI 630 GCATC GCATC(5/9)
905 GCATC
SspI 869 AATATT AAT^ATT
978 AATATT
StyI 551 CCAAGG C^C[AT][AT]GG
924 CCAAGG
Tsp509I 138 AATT ^AATT
329 AATT
Van91I 102 CCAGGGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1279 CCAGAAGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AciI AfaI AluI AlwI BbvI
BclI BfaI BmyI BpmI BsaJI
BsiYI BslI BsmFI Bsp1286I BssKI
BstF5I BstNI Cac8I DdeI DpnII
EcoRII FbaI Fnu4HI FokI HaeIII
HinfI HphI ItaI MaeI MaeIII
MboI MboII MnlI MseI MslI
MvaI NdeII NlaIII NspI PleI
RsaI Sau3AI ScrFI SfcI Tru9I
TseI Tsp45I TspRI