################################## # NO CUT ENZYMES ( 45 enzymes) # ################################## AatII AclI AflII AscI AseI AsnI BcgI BfrI BglII BsiWI BspDI BspHI BstBI ClaI DraI EagI EclXI Eco52I EcoNI EcoRI FseI HindIII KspI MluI NheI NotI NruI NspV PacI PmeI PshBI Psp1406I PvuI RcaI SacII SalI SexAI SfiI SfuI SnaBI SpeI SphI SspI SwaI XbaI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AgeI 3750 ACCGGT A^CCGGT AhdI 5527 GACACGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 5111 GTGCAC G^TGCAC AocI 2672 CCTCAGG CC^T[ACGT]AGG ApaI 1625 GGGCCC GGGCC^C ApaLI 5111 GTGCAC G^TGCAC AspEI 5527 GACACGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 4716 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC BamHI 1472 GGATCC G^GATCC BciVI 2433 GGATAC GTATCC(6/5) BclI 4394 TGATCA T^GATCA BsiEI 1284 CGACCG CG[GA][CT]^CG BssHII 17 GCGCGC G^CGCGC BssSI 3862 CTCGTG CACGAG(-5/-1) BstEII 2475 GGTCACC G^GT[ACGT]ACC BstPI 2475 GGTCACC G^GT[ACGT]ACC Bsu36I 2672 CCTCAGG CC^T[ACGT]AGG CpoI 3580 CGGTCCG CG^G[AT]CCG DraIII 3011 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG Eam1105I 5527 GACACGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 2672 CCTCAGG CC^T[ACGT]AGG EcoO65I 2475 GGTCACC G^GT[ACGT]ACC EcoRV 4795 GATATC GAT^ATC EcoT22I 5514 ATGCAT ATGCA^T FbaI 4394 TGATCA T^GATCA HpaI 1078 GTTAAC GTT^AAC MfeI 2777 CAATTG C^AATTG MunI 2777 CAATTG C^AATTG NdeI 3764 CATATG CA^TATG NsiI 5514 ATGCAT ATGCA^T PflFI 4716 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC PinAI 3750 ACCGGT A^CCGGT RsrII 3580 CGGTCCG CG^G[AT]CCG SacI 4147 GAGCTC GAGCT^C SbfI 2086 CCTGCAGG CCTGCA^GG SgrAI 3749 CACCGGTG C[GA]^CCGG[CT]G Sse8387I 2086 CCTGCAGG CCTGCA^GG Tth111I 4716 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 39 enzymes) # ################################### AccIII 368 TCCGGA T^CCGGA 3583 TCCGGA AcsI 2002 AAATTT [GA]^AATT[CT] 2782 GAATTT Aor51HI 1993 AGCGCT AGC^GCT 2908 AGCGCT ApoI 2002 AAATTT [GA]^AATT[CT] 2782 GAATTT AvrII 3627 CCTAGG C^CTAGG 4966 CCTAGG BbeI 31 GGCGCC GGCGC^C 1826 GGCGCC BbrPI 1173 CACGTG CAC^GTG 2356 CACGTG BbvCI 548 CCTCAGC CCTCAGC(-5/-2) 4813 CCTCAGC BlnI 3627 CCTAGG C^CTAGG 4966 CCTAGG BlpI 484 GCTGAGC GC^T[ACGT]AGC 1849 GCTAAGC Bpu1102I 484 GCTGAGC GC^T[ACGT]AGC 1849 GCTAAGC BsaAI 1173 CACGTG [CT]AC^GT[GA] 2356 CACGTG BsaI 4057 GAGACC GGTCTC(1/5) 3183 GGTCTC BseAI 368 TCCGGA T^CCGGA 3583 TCCGGA BsmBI 5054 CGTCTC CGTCTC(1/5) 5572 GAGACG BsmI 1404 GAATGC GAATGC(1/-1) 3564 GAATGC Bsp1407I 5228 TGTACA T^GTACA 5348 TGTACA BspEI 368 TCCGGA T^CCGGA 3583 TCCGGA BspLU11I 97 ACATGT A^CATGT 2615 ACATGT BsrGI 5228 TGTACA T^GTACA 5348 TGTACA Bst1107I 197 GTATAC GTA^TAC 3412 GTATAC BstXI 624 CCACTGCGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2838 CCACCCATTTGG BstZ17I 197 GTATAC GTA^TAC 3412 GTATAC CelII 484 GCTGAGC GC^T[ACGT]AGC 1849 GCTAAGC DrdI 2971 GACATCCATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 5046 GACCCGCACGTC Eco47III 1993 AGCGCT AGC^GCT 2908 AGCGCT KasI 31 GGCGCC G^GCGCC 1826 GGCGCC MroI 368 TCCGGA T^CCGGA 3583 TCCGGA NarI 31 GGCGCC GG^CGCC 1826 GGCGCC NcoI 1688 CCATGG C^CATGG 2603 CCATGG PaeR7I 1596 CTCGAG C^TCGAG 3942 CTCGAG PmaCI 1173 CACGTG CAC^GTG 2356 CACGTG PmlI 1173 CACGTG CAC^GTG 2356 CACGTG SapI 5593 GAAGAGC GCTCTTC(1/4) 4068 GCTCTTC ScaI 2553 AGTACT AGT^ACT 4389 AGTACT SfoI 31 GGCGCC GGC^GCC 1826 GGCGCC SspBI 5228 TGTACA T^GTACA 5348 TGTACA StuI 1739 AGGCCT AGG^CCT 3882 AGGCCT XhoI 1596 CTCGAG C^TCGAG 3942 CTCGAG ######################################### # 3 OR MORE CUT ENZYMES ( 136 enzymes) # ######################################### Acc65I AccI AccII AciI AcyI AfaI AflIII AluI AlwI AlwNI Asp700I Asp718I AspHI AvaI AvaII AviII BalI BanI BanII BbsI BbvI BcnI BfaI BglI BmyI BpmI BpuAI BsaBI BsaHI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrDI BsrFI BsrI BssKI BstAPI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI FspI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI KpnI Ksp632I MaeI MaeII MaeIII MamI MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NaeI NciI NdeII NgoMIV NlaIII NlaIV NspI PflMI PleI PpuMI PshAI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII XmaCI XmaI XmnI