Restriction map for
pF1KA0808
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# NO CUT ENZYMES ( 125 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AhdI
AocI Aor51HI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaI AviII AvrII BanI BbeI
BbrPI BcgI BfrI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsaHI
BseAI BsiEI BsiWI BsmBI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BsrGI BssHII BssSI Bst1107I BstAPI
BstUI BstZ17I Bsu36I CelII ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoRI EcoRV FseI FspI
HaeII Hin1I HindIII HpaI KasI
KpnI KspI MamI MfeI MluI
MroI MunI MvnI NaeI NarI
NdeI NgoMIV NheI NotI NruI
PacI PaeR7I PflFI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SbfI ScaI SexAI SfoI SgrAI
SmaI SmlI SnaBI SpeI Sse8387I
SspBI SspI StuI SwaI Tth111I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 61 enzymes) #
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AflIII 116 ACATGT A^C[GA][CT]GT
AgeI 1509 ACCGGT A^CCGGT
Alw44I 250 GTGCAC G^TGCAC
AlwNI 1214 CAGTGACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 1081 GGGCCC GGGCC^C
ApaLI 250 GTGCAC G^TGCAC
BalI 528 TGGCCA TGG^CCA
BamHI 768 GGATCC G^GATCC
BbvCI 1045 CCTCAGC CCTCAGC(-5/-2)
BciVI 997 GTATCC GTATCC(6/5)
BclI 1052 TGATCA T^GATCA
BcnI 1135 CCGGG CC^[GC]GG
BfaI 1169 CTAG C^TAG
BglI 1093 GCCCACTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaI 474 GAGACC GGTCTC(1/5)
BsaWI 1509 ACCGGT [AT]^CCGG[AT]
BspLU11I 116 ACATGT A^CATGT
BspMI 1353 GCAGGT ACCTGC(4/8)
BsrBI 1312 CCGCTC CCGCTC(-3/-3)
BsrFI 1509 ACCGGT [GA]^CCGG[CT]
BstBI 1059 TTCGAA TT^CGAA
BstEII 274 GGTTACC G^GT[ACGT]ACC
BstPI 274 GGTTACC G^GT[ACGT]ACC
BstXI 362 CCAATATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 768 GGATCC [GA]^GATC[CT]
CfoI 43 GCGC GCG^C
Cfr10I 1509 ACCGGT [GA]^CCGG[CT]
DraII 63 GGGACCT [GA]G^G[ACGT]CC[CT]
DsaI 1077 CCATGG C^C[GA][CT]GG
EaeI 528 TGGCCA [CT]^GGCC[GA]
EcoO109I 63 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoO65I 274 GGTTACC G^GT[ACGT]ACC
EcoT22I 1162 ATGCAT ATGCA^T
FbaI 1052 TGATCA T^GATCA
HgaI 49 GACGC GACGC(5/10)
HhaI 43 GCGC GCG^C
HinP1I 43 GCGC G^CGC
HincII 1021 GTTGAC GT[CT]^[GA]AC
HindII 1021 GTTGAC GT[CT]^[GA]AC
MaeI 1169 CTAG C^TAG
MflI 768 GGATCC [GA]^GATC[CT]
MluNI 528 TGGCCA TGG^CCA
MscI 528 TGGCCA TGG^CCA
MseI 545 TTAA T^TAA
NciI 1135 CCGGG CC^[GC]GG
NcoI 1077 CCATGG C^CATGG
NsiI 1162 ATGCAT ATGCA^T
NspV 1059 TTCGAA TT^CGAA
PinAI 1509 ACCGGT A^CCGGT
PleI 268 GAGTC GAGTC(4/5)
PpuMI 63 GGGACCT [GA]G^G[AT]CC[CT]
SapI 910 GAAGAGC GCTCTTC(1/4)
SfiI 1092 GGCCCACTCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 1059 TTCGAA TT^CGAA
SphI 1547 GCATGC GCATG^C
TaqI 1060 TCGA T^CGA
TfiI 1143 GAATC G^A[AT]TC
Tru9I 545 TTAA T^TAA
TthHB8I 1060 TCGA T^CGA
XcmI 165 CCAGTCCTACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 768 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AlwI 769 GATCC GGATC(4/5)
768 GGATC
AspHI 250 GTGCAC G[AT]GC[AT]^C
563 GTGCTC
BanII 778 GAGCCC G[GA]GC[CT]^C
1081 GGGCCC
BbsI 196 GAAGAC GAAGAC(2/6)
1029 GAAGAC
BpmI 23 CTCCAG CTGGAG(16/14)
1261 CTCCAG
BpuAI 196 GAAGAC GAAGAC(2/6)
1029 GAAGAC
BsgI 1445 CTGCAC GTGCAG(16/14)
1488 GTGCAG
BsiHKAI 250 GTGCAC G[AT]GC[AT]^C
563 GTGCTC
BsrDI 593 GCAATG GCAATG(2/0)
1465 GCAATG
FauI 34 CCCGC CCCGC(4/6)
1272 CCCGC
HapII 1135 CCGG C^CGG
1510 CCGG
HinfI 268 GAGTC G^A[ACGT]TC
1143 GAATC
HpaII 1135 CCGG C^CGG
1510 CCGG
MaeII 5 ACGT A^CGT
1025 ACGT
MspI 1135 CCGG C^CGG
1510 CCGG
PflMI 240 CCACTCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1461 CCAGGCAATGG
PstI 491 CTGCAG CTGCA^G
1533 CTGCAG
Van91I 240 CCACTCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1461 CCAGGCAATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AciI AfaI AluI AvaII BbvI
BmyI BsaJI BseRI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII EarI EcoRII EcoT14I Fnu4HI
FokI HaeIII HphI ItaI Ksp632I
MaeIII MboI MboII MnlI MslI
MspA1I MvaI MwoI NdeII NlaIII
NlaIV NspI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TseI Tsp45I Tsp509I TspRI