Restriction map for
pF1KA0774
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# NO CUT ENZYMES ( 69 enzymes) #
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AatII Acc65I AccI AclI AgeI
AhdI Aor51HI AseI AsnI Asp700I
Asp718I AspEI AvrII BbeI BbrPI
BcgI BclI BlnI BsaAI BsaBI
BsiWI Bsp1407I BspDI BsrGI Bst1107I
BstZ17I ClaI CpoI Eam1105I Eco47III
EcoRI EcoRV EcoT22I FbaI HpaI
KasI KpnI MamI MfeI MluI
MunI NarI NcoI NheI NotI
NruI NsiI PacI PaeR7I PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RsrII SalI ScaI SfiI
SfoI SgrAI SnaBI SpeI SphI
SspBI SwaI XhoI XmnI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AcyI 2005 GACGCC G[GA]^CG[CT]C
AocI 1235 CCTTAGG CC^T[ACGT]AGG
ApaI 4099 GGGCCC GGGCC^C
AscI 3366 GGCGCGCC GG^CGCGCC
AspI 416 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 3072 TGCGCA TGC^GCA
BanI 1476 GGTGCC G^G[CT][GA]CC
BbvCI 1595 CCTCAGC CCTCAGC(-5/-2)
BglII 3435 AGATCT A^GATCT
BsaHI 2005 GACGCC G[GA]^CG[CT]C
BsiEI 2748 CGGCCG CG[GA][CT]^CG
BssHII 3367 GCGCGC G^CGCGC
BssSI 3790 CACGAG CACGAG(-5/-1)
BstAPI 3015 GCAGCTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 3667 TTCGAA TT^CGAA
Bsu36I 1235 CCTTAGG CC^T[ACGT]AGG
DraI 2481 TTTAAA TTT^AAA
EagI 2748 CGGCCG C^GGCCG
EclXI 2748 CGGCCG C^GGCCG
Eco52I 2748 CGGCCG C^GGCCG
Eco81I 1235 CCTTAGG CC^T[ACGT]AGG
FspI 3072 TGCGCA TGC^GCA
Hin1I 2005 GACGCC G[GA]^CG[CT]C
KspI 4083 CCGCGG CCGC^GG
MslI 1923 CATCATTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NdeI 2033 CATATG CA^TATG
NspV 3667 TTCGAA TT^CGAA
PflFI 416 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 3926 GACAGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 4083 CCGCGG CCGC^GG
SfuI 3667 TTCGAA TT^CGAA
SspI 1621 AATATT AAT^ATT
StuI 3674 AGGCCT AGG^CCT
Tth111I 416 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 506 CCAAGGATAAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 41 enzymes) #
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AccIII 2161 TCCGGA T^CCGGA
2594 TCCGGA
AflII 117 CTTAAG C^TTAAG
2326 CTTAAG
Alw44I 1760 GTGCAC G^TGCAC
2642 GTGCAC
ApaLI 1760 GTGCAC G^TGCAC
2642 GTGCAC
BalI 1110 TGGCCA TGG^CCA
3122 TGGCCA
BamHI 1068 GGATCC G^GATCC
1817 GGATCC
BciVI 2548 GGATAC GTATCC(6/5)
2935 GGATAC
BfrI 117 CTTAAG C^TTAAG
2326 CTTAAG
BglI 2569 GCCAAACTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2998 GCCCGTGAGGC
BlpI 3007 GCTGAGC GC^T[ACGT]AGC
3399 GCTGAGC
Bpu1102I 3007 GCTGAGC GC^T[ACGT]AGC
3399 GCTGAGC
BsaI 802 GAGACC GGTCTC(1/5)
2533 GGTCTC
BseAI 2161 TCCGGA T^CCGGA
2594 TCCGGA
BsgI 837 GTGCAG GTGCAG(16/14)
1280 GTGCAG
BsmBI 2557 CGTCTC CGTCTC(1/5)
2607 CGTCTC
BspEI 2161 TCCGGA T^CCGGA
2594 TCCGGA
BspHI 171 TCATGA T^CATGA
3549 TCATGA
BsrFI 2660 GCCGGC [GA]^CCGG[CT]
2746 GCCGGC
BstXI 1134 CCACCACCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3056 CCAGACAGCTGG
CelII 3007 GCTGAGC GC^T[ACGT]AGC
3399 GCTGAGC
Cfr10I 2660 GCCGGC [GA]^CCGG[CT]
2746 GCCGGC
DrdI 694 GACCACGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3520 GACCACAAAGTC
DsaI 3119 CCGTGG C^C[GA][CT]GG
4083 CCGCGG
FseI 2659 GGCCGGCC GGCCGG^CC
2745 GGCCGGCC
HaeII 2413 AGCGCC [GA]GCGC^[CT]
2808 AGCGCC
HindIII 2489 AAGCTT A^AGCTT
2920 AAGCTT
MluNI 1110 TGGCCA TGG^CCA
3122 TGGCCA
MroI 2161 TCCGGA T^CCGGA
2594 TCCGGA
MscI 1110 TGGCCA TGG^CCA
3122 TGGCCA
NaeI 2660 GCCGGC GCC^GGC
2746 GCCGGC
NgoMIV 2660 GCCGGC G^CCGGC
2746 GCCGGC
RcaI 171 TCATGA T^CATGA
3549 TCATGA
SacI 2598 GAGCTC GAGCT^C
3316 GAGCTC
SapI 358 GAAGAGC GCTCTTC(1/4)
4009 GAAGAGC
SbfI 3372 CCTGCAGG CCTGCA^GG
3504 CCTGCAGG
SexAI 1323 ACCAGGT A^CC[AT]GGT
3611 ACCAGGT
SmaI 635 CCCGGG CCC^GGG
2190 CCCGGG
Sse8387I 3372 CCTGCAGG CCTGCA^GG
3504 CCTGCAGG
XbaI 459 TCTAGA T^CTAGA
1566 TCTAGA
XmaCI 635 CCCGGG C^CCGGG
2190 CCCGGG
XmaI 635 CCCGGG C^CCGGG
2190 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI AspHI
AvaI AvaII BanII BbsI BbvI
BcnI BfaI BmyI BpmI BpuAI
BsaJI BsaWI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I BspLU11I BspMI BsrBI BsrDI
BsrI BssKI BstEII BstF5I BstNI
BstPI BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII DraIII
EaeI EarI EcoNI EcoO109I EcoO65I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XhoII