Restriction map for
pF1KA0766
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# NO CUT ENZYMES ( 109 enzymes) #
##################################
Acc65I AclI AflII AflIII AgeI
AhdI Alw44I AlwNI Aor51HI ApaLI
AscI AseI AsnI Asp718I AspEI
AviII AvrII BalI BbrPI BbsI
BbvCI BcgI BciVI BclI BfrI
BglI BlnI BpuAI BsaAI BsaI
BseRI BsiEI BsiWI BsmI Bsp1407I
BspDI BspHI BspLU11I BspMI BsrDI
BsrFI BsrGI BstAPI BstEII BstPI
BstXI Cfr10I ClaI CpoI DraIII
DrdI EaeI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO65I EcoRI
EcoT22I FbaI FseI FspI HindIII
HpaI KpnI MluI MluNI MscI
NaeI NcoI NdeI NgoMIV NheI
NotI NruI NsiI PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SapI SbfI ScaI SexAI
SfiI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SwaI
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AatII 157 GACGTC GACGT^C
AccIII 93 TCCGGA T^CCGGA
AocI 1431 CCTCAGG CC^T[ACGT]AGG
ApaI 107 GGGCCC GGGCC^C
AspI 1004 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 458 GGATCC G^GATCC
BanI 164 GGCGCC G^G[CT][GA]CC
BbeI 164 GGCGCC GGCGC^C
BcnI 1777 CCCGG CC^[GC]GG
BglII 618 AGATCT A^GATCT
BpmI 437 CTCCAG CTGGAG(16/14)
BsaWI 93 TCCGGA [AT]^CCGG[AT]
BseAI 93 TCCGGA T^CCGGA
BsgI 296 GTGCAG GTGCAG(16/14)
BspEI 93 TCCGGA T^CCGGA
BsrBI 196 GAGCGG CCGCTC(-3/-3)
BsrI 366 CCAGT ACTGG(1/-1)
BssHII 219 GCGCGC G^CGCGC
BssSI 293 CTCGTG CACGAG(-5/-1)
Bst1107I 361 GTATAC GTA^TAC
BstBI 1243 TTCGAA TT^CGAA
BstZ17I 361 GTATAC GTA^TAC
Bsu36I 1431 CCTCAGG CC^T[ACGT]AGG
EarI 280 GAAGAG CTCTTC(1/4)
Eco81I 1431 CCTCAGG CC^T[ACGT]AGG
EcoRV 442 GATATC GAT^ATC
EcoT14I 533 CCTTGG C^C[AT][AT]GG
FauI 146 CCCGC CCCGC(4/6)
HaeII 164 GGCGCC [GA]GCGC^[CT]
KasI 164 GGCGCC G^GCGCC
Ksp632I 280 GAAGAG CTCTTC(1/4)
KspI 585 CCGCGG CCGC^GG
MaeII 158 ACGT A^CGT
MfeI 1017 CAATTG C^AATTG
MroI 93 TCCGGA T^CCGGA
MspA1I 585 CCGCGG C[AC]G^C[GT]G
MunI 1017 CAATTG C^AATTG
NarI 164 GGCGCC GG^CGCC
NciI 1777 CCCGG CC^[GC]GG
NspI 405 GCATGT [GA]CATG^[CT]
NspV 1243 TTCGAA TT^CGAA
PflFI 1004 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 1427 AGGACCT [GA]G^G[AT]CC[CT]
PstI 691 CTGCAG CTGCA^G
SacI 861 GAGCTC GAGCT^C
SacII 585 CCGCGG CCGC^GG
SalI 34 GTCGAC G^TCGAC
SfcI 691 CTGCAG C^T[GA][CT]AG
SfoI 164 GGCGCC GGC^GCC
SfuI 1243 TTCGAA TT^CGAA
StuI 300 AGGCCT AGG^CCT
StyI 533 CCTTGG C^C[AT][AT]GG
Tsp45I 1136 GTGAC ^GT[GC]AC
Tth111I 1004 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 1760 CCACAACTGAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccI 34 GTCGAC GT^[AC][GT]AC
361 GTATAC
AfaI 394 GTAC GT^AC
1238 GTAC
Asp700I 616 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1165 GAACACCTTC
AspHI 181 GAGCAC G[AT]GC[AT]^C
861 GAGCTC
AvaII 65 GGACC G^G[AT]CC
1428 GGACC
BfaI 46 CTAG C^TAG
682 CTAG
BlpI 178 GCTGAGC GC^T[ACGT]AGC
705 GCTTAGC
Bpu1102I 178 GCTGAGC GC^T[ACGT]AGC
705 GCTTAGC
BsiHKAI 181 GAGCAC G[AT]GC[AT]^C
861 GAGCTC
BsmBI 777 CGTCTC CGTCTC(1/5)
1396 CGTCTC
BstYI 458 GGATCC [GA]^GATC[CT]
618 AGATCT
CelII 178 GCTGAGC GC^T[ACGT]AGC
705 GCTTAGC
DsaI 260 CCGTGG C^C[GA][CT]GG
585 CCGCGG
HincII 34 GTCGAC GT[CT]^[GA]AC
427 GTTGAC
HindII 34 GTCGAC GT[CT]^[GA]AC
427 GTTGAC
MaeI 46 CTAG C^TAG
682 CTAG
MaeIII 1136 GTGAC ^GT[ACGT]AC
1658 GTAAC
MflI 458 GGATCC [GA]^GATC[CT]
618 AGATCT
MslI 372 CATGGAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
649 CATTTCAGTG
PflMI 366 CCAGTGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
809 CCAACTGTTGG
RsaI 394 GTAC GT^AC
1238 GTAC
SfaNI 1642 GCATC GCATC(5/9)
1734 GCATC
SspI 1050 AATATT AAT^ATT
1359 AATATT
Van91I 366 CCAGTGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
809 CCAACTGTTGG
XhoII 458 GGATCC [GA]^GATC[CT]
618 AGATCT
XmnI 616 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1165 GAACACCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AccII AciI AcsI AcyI AluI
AlwI ApoI AvaI BanII BbvI
BmyI BsaBI BsaHI BsaJI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr13I DdeI DpnII DraI
DraII EcoO109I EcoRII Fnu4HI FokI
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
MamI MboI MboII MnlI MseI
MspI MvaI MvnI MwoI NdeII
NlaIII NlaIV PleI Sau3AI Sau96I
ScrFI TaqI TfiI Tru9I TseI
Tsp509I TspRI TthHB8I