Restriction map for
pF1KA0765
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# NO CUT ENZYMES ( 124 enzymes) #
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AatII Acc65I AccI AccII AccIII
AcyI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvrII BalI BamHI BbeI BbrPI
BbsI BbvCI BcgI BclI BfrI
BglII BlnI BlpI Bpu1102I BpuAI
BsaBI BsaHI BsaWI BseAI BsiEI
BsiWI BsmBI BspDI BspEI BsrBI
BsrFI BssHII BssSI Bst1107I BstEII
BstPI BstUI BstYI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DrdI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI HaeII HgaI
Hin1I KasI KpnI KspI MamI
MflI MluI MluNI MroI MscI
MvnI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI PvuI RsrII
SacI SacII SalI SbfI ScaI
SexAI SfiI SfoI SgrAI SnaBI
SpeI Sse8387I SwaI Tsp45I Tth111I
XbaI XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AclI 507 AACGTT AA^CGTT
AlwNI 2732 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 2361 GGGCCC GGGCC^C
AspHI 788 GAGCAC G[AT]GC[AT]^C
AviII 173 TGCGCA TGC^GCA
BanI 756 GGCACC G^G[CT][GA]CC
BciVI 367 GTATCC GTATCC(6/5)
BsaAI 1793 CACGTA [CT]AC^GT[GA]
BsaI 25 GGTCTC GGTCTC(1/5)
BsiHKAI 788 GAGCAC G[AT]GC[AT]^C
Bsp1407I 1719 TGTACA T^GTACA
BspHI 420 TCATGA T^CATGA
BspMI 765 ACCTGC ACCTGC(4/8)
BsrGI 1719 TGTACA T^GTACA
BstBI 1550 TTCGAA TT^CGAA
BstXI 2514 CCATATTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 174 GCGC GCG^C
DraIII 389 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 2107 GAAGAG CTCTTC(1/4)
FspI 173 TGCGCA TGC^GCA
HhaI 174 GCGC GCG^C
HinP1I 174 GCGC G^CGC
HpaI 847 GTTAAC GTT^AAC
Ksp632I 2107 GAAGAG CTCTTC(1/4)
MfeI 32 CAATTG C^AATTG
MspA1I 2732 CAGCTG C[AC]G^C[GT]G
MunI 32 CAATTG C^AATTG
NspV 1550 TTCGAA TT^CGAA
PflMI 2467 CCAGCTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1185 GGGACCT [GA]G^G[AT]CC[CT]
Psp1406I 507 AACGTT AA^CGTT
PvuII 2732 CAGCTG CAG^CTG
RcaI 420 TCATGA T^CATGA
SapI 2107 GAAGAGC GCTCTTC(1/4)
SfuI 1550 TTCGAA TT^CGAA
SmaI 2406 CCCGGG CCC^GGG
SmlI 1058 CTCAAG C^T[CT][GA]AG
SspBI 1719 TGTACA T^GTACA
SspI 1648 AATATT AAT^ATT
Van91I 2467 CCAGCTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 60 CCACTTCTTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 2406 CCCGGG C^CCGGG
XmaI 2406 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AcsI 798 GAATTT [GA]^AATT[CT]
2370 GAATTT
AflIII 1332 ACATGT A^C[GA][CT]GT
1722 ACATGT
AlwI 2131 GGATC GGATC(4/5)
2185 GGATC
ApoI 798 GAATTT [GA]^AATT[CT]
2370 GAATTT
AvaI 2406 CCCGGG C^[CT]CG[GA]G
2717 CTCGGG
BglI 1239 GCCCAGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2698 GCCATGGTGGC
BsmAI 26 GTCTC GTCTC(1/5)
2715 GTCTC
BsoBI 2406 CCCGGG C^[CT]CG[GA]G
2717 CTCGGG
BspLU11I 1332 ACATGT A^CATGT
1722 ACATGT
BsrDI 647 CATTGC GCAATG(2/0)
943 GCAATG
BstAPI 924 GCATGGAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2580 GCAAAACATGC
DsaI 47 CCATGG C^C[GA][CT]GG
2699 CCATGG
HincII 847 GTTAAC GT[CT]^[GA]AC
1615 GTCAAC
HindII 847 GTTAAC GT[CT]^[GA]AC
1615 GTCAAC
HindIII 1073 AAGCTT A^AGCTT
1838 AAGCTT
MaeII 508 ACGT A^CGT
1794 ACGT
MslI 2452 CATTTGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2515 CATATTGGTG
NcoI 47 CCATGG C^CATGG
2699 CCATGG
PleI 255 GAGTC GAGTC(4/5)
2713 GAGTC
PstI 1561 CTGCAG CTGCA^G
1940 CTGCAG
SphI 728 GCATGC GCATG^C
2112 GCATGC
TaqI 1020 TCGA T^CGA
1551 TCGA
TthHB8I 1020 TCGA T^CGA
1551 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AciI AfaI AluI AvaII BanII
BbvI BcnI BfaI BmyI BpmI
BsaJI BseRI BsgI BsiYI BslI
BsmFI BsmI Bsp1286I BsrI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII DraI DraII EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI MaeI MaeIII MboI MboII
MnlI MseI MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI StuI StyI TfiI Tru9I
TseI Tsp509I TspRI