Restriction map for
pF1KA0725
##################################
# NO CUT ENZYMES ( 131 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflIII AgeI AhdI Alw44I
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AspI AvaI
AviII AvrII BalI BamHI BanI
BbeI BbrPI BbvCI BcgI BglI
BlnI BlpI BpmI Bpu1102I BsaHI
BsaWI BseAI BsiEI BsiWI BsoBI
BspDI BspEI BspHI BspLU11I BsrBI
BsrFI BssHII BssSI BstAPI BstEII
BstPI BstUI CelII CfoI Cfr10I
ClaI CpoI DraI DraII DraIII
DrdI EaeI EagI Eam1105I EclXI
Eco47III Eco52I EcoO109I EcoO65I EcoT22I
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I KasI KpnI KspI
MfeI MluI MluNI MroI MscI
MunI MvnI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SapI SbfI ScaI SexAI
SfiI SfoI SgrAI SmaI SnaBI
SpeI SphI Sse8387I StuI SwaI
Tth111I Van91I XbaI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AflII 1756 CTTAAG C^TTAAG
AocI 885 CCTCAGG CC^T[ACGT]AGG
Asp700I 796 GAATTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 1808 GAGCTC G[AT]GC[AT]^C
BanII 1808 GAGCTC G[GA]GC[CT]^C
BbsI 919 GTCTTC GAAGAC(2/6)
BciVI 1033 GTATCC GTATCC(6/5)
BclI 2118 TGATCA T^GATCA
BcnI 1478 CCCGG CC^[GC]GG
BfrI 1756 CTTAAG C^TTAAG
BmyI 1808 GAGCTC G[AGT]GC[ACT]^C
BpuAI 919 GTCTTC GAAGAC(2/6)
BsaAI 339 TACGTG [CT]AC^GT[GA]
BsaBI 844 GATGTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 587 GAGGAG GAGGAG(10/8)
BsgI 10 GTGCAG GTGCAG(16/14)
BsiHKAI 1808 GAGCTC G[AT]GC[AT]^C
BsmBI 330 GAGACG CGTCTC(1/5)
BsmI 828 GCATTC GAATGC(1/-1)
Bsp1286I 1808 GAGCTC G[AGT]GC[ACT]^C
BspMI 1458 GCAGGT ACCTGC(4/8)
BsrDI 1038 CATTGC GCAATG(2/0)
Bst1107I 301 GTATAC GTA^TAC
BstBI 692 TTCGAA TT^CGAA
BstZ17I 301 GTATAC GTA^TAC
Bsu36I 885 CCTCAGG CC^T[ACGT]AGG
Eco81I 885 CCTCAGG CC^T[ACGT]AGG
EcoNI 1369 CCTGCTTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 1362 CCCGC CCCGC(4/6)
FbaI 2118 TGATCA T^GATCA
HapII 1479 CCGG C^CGG
HpaI 1894 GTTAAC GTT^AAC
HpaII 1479 CCGG C^CGG
MamI 844 GATGTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI 1524 CATTGGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 196 CAGCTG C[AC]G^C[GT]G
MspI 1479 CCGG C^CGG
NciI 1478 CCCGG CC^[GC]GG
NspI 419 ACATGC [GA]CATG^[CT]
NspV 692 TTCGAA TT^CGAA
PleI 2056 GAGTC GAGTC(4/5)
PvuII 196 CAGCTG CAG^CTG
SacI 1808 GAGCTC GAGCT^C
SalI 566 GTCGAC G^TCGAC
SfuI 692 TTCGAA TT^CGAA
SmlI 1756 CTTAAG C^T[CT][GA]AG
XcmI 1739 CCAGGATGAGTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 796 GAATTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AccI 301 GTATAC GT^[AC][GT]AC
566 GTCGAC
BsaI 1353 GAGACC GGTCTC(1/5)
1906 GAGACC
Bsp1407I 702 TGTACA T^GTACA
1255 TGTACA
BsrGI 702 TGTACA T^GTACA
1255 TGTACA
BstXI 660 CCATGGGATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
805 CCAGTCAACTGG
DsaI 554 CCATGG C^C[GA][CT]GG
660 CCATGG
EcoRI 1201 GAATTC G^AATTC
1283 GAATTC
EcoRV 1075 GATATC GAT^ATC
1210 GATATC
EcoT14I 554 CCATGG C^C[AT][AT]GG
660 CCATGG
HindIII 499 AAGCTT A^AGCTT
1829 AAGCTT
MaeII 340 ACGT A^CGT
1585 ACGT
NcoI 554 CCATGG C^CATGG
660 CCATGG
NlaIV 1010 GGAACC GG[ACGT]^[ACGT]CC
1653 GGTCCC
PstI 853 CTGCAG CTGCA^G
1407 CTGCAG
SspBI 702 TGTACA T^GTACA
1255 TGTACA
SspI 1135 AATATT AAT^ATT
1603 AATATT
StyI 554 CCATGG C^C[AT][AT]GG
660 CCATGG
TaqI 567 TCGA T^CGA
693 TCGA
TthHB8I 567 TCGA T^CGA
693 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AvaII BbvI BfaI
BglII BsaJI BsiYI BslI BsmAI
BsmFI BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
EarI EcoRII Fnu4HI FokI HaeIII
HincII HindII HinfI HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MnlI MseI MvaI MwoI
NdeII NlaIII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI XhoII