Restriction map for
pF1KA0713
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# NO CUT ENZYMES ( 111 enzymes) #
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AatII Acc65I AccII AccIII AcyI
AflII AgeI AhdI Aor51HI ApaI
AscI Asp718I AspEI AspI AvaI
AvrII BanII BbeI BbrPI BbvCI
BcgI BfrI BglI BlnI BsaAI
BsaBI BsaHI BsaWI BseAI BsgI
BsiEI BsiWI BsmBI BsoBI BspDI
BspEI BspMI BsrBI BsrDI BssHII
Bst1107I BstUI BstXI BstZ17I ClaI
CpoI DraI DraII DsaI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoO109I FauI FseI HgaI Hin1I
HindIII KasI KpnI KspI MamI
MfeI MluI MroI MunI MvnI
NaeI NarI NcoI NgoMIV NheI
NotI NruI PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PstI PvuI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SmaI
SnaBI SpeI SphI Sse8387I SwaI
Tth111I Van91I XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 67 enzymes) #
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AccI 1135 GTAGAC GT^[AC][GT]AC
AciI 1235 CCGC CCGC(-3/-1)
AclI 2504 AACGTT AA^CGTT
AflIII 1550 ACATGT A^C[GA][CT]GT
Alw44I 639 GTGCAC G^TGCAC
AocI 669 CCTAAGG CC^T[ACGT]AGG
ApaLI 639 GTGCAC G^TGCAC
AspHI 639 GTGCAC G[AT]GC[AT]^C
AvaII 73 GGACC G^G[AT]CC
AviII 1529 TGCGCA TGC^GCA
BalI 2524 TGGCCA TGG^CCA
BamHI 1576 GGATCC G^GATCC
BbsI 2728 GTCTTC GAAGAC(2/6)
BciVI 1222 GGATAC GTATCC(6/5)
BcnI 1227 CCGGG CC^[GC]GG
BlpI 1540 GCTGAGC GC^T[ACGT]AGC
BmyI 639 GTGCAC G[AGT]GC[ACT]^C
Bpu1102I 1540 GCTGAGC GC^T[ACGT]AGC
BpuAI 2728 GTCTTC GAAGAC(2/6)
BsaI 1610 GGTCTC GGTCTC(1/5)
BseRI 1777 GAGGAG GAGGAG(10/8)
BsiHKAI 639 GTGCAC G[AT]GC[AT]^C
BsmFI 1114 GTCCC GGGAC(10/14)
Bsp1286I 639 GTGCAC G[AGT]GC[ACT]^C
Bsp1407I 1752 TGTACA T^GTACA
BspHI 1419 TCATGA T^CATGA
BspLU11I 1550 ACATGT A^CATGT
BsrFI 1757 ACCGGC [GA]^CCGG[CT]
BsrGI 1752 TGTACA T^GTACA
BssSI 764 CACGAG CACGAG(-5/-1)
BstAPI 1735 GCATTATATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 2573 TTCGAA TT^CGAA
BstEII 1230 GGTTACC G^GT[ACGT]ACC
BstPI 1230 GGTTACC G^GT[ACGT]ACC
Bsu36I 669 CCTAAGG CC^T[ACGT]AGG
Cac8I 2056 GCTCGC GC[ACGT]^[ACGT]GC
CelII 1540 GCTGAGC GC^T[ACGT]AGC
Cfr10I 1757 ACCGGC [GA]^CCGG[CT]
Cfr13I 73 GGACC G^G[ACGT]CC
DraIII 1999 CACTATGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2154 GACTAAAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 2524 TGGCCA [CT]^GGCC[GA]
EarI 407 CTCTTC CTCTTC(1/4)
Eco81I 669 CCTAAGG CC^T[ACGT]AGG
EcoO65I 1230 GGTTACC G^GT[ACGT]ACC
EcoRI 770 GAATTC G^AATTC
EcoRV 976 GATATC GAT^ATC
EcoT14I 42 CCTTGG C^C[AT][AT]GG
FspI 1529 TGCGCA TGC^GCA
HaeII 2850 AGCGCC [GA]GCGC^[CT]
HpaI 3 GTTAAC GTT^AAC
Ksp632I 407 CTCTTC CTCTTC(1/4)
MaeII 2505 ACGT A^CGT
MluNI 2524 TGGCCA TGG^CCA
MscI 2524 TGGCCA TGG^CCA
MslI 828 CATATGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NciI 1227 CCGGG CC^[GC]GG
NspV 2573 TTCGAA TT^CGAA
Psp1406I 2504 AACGTT AA^CGTT
RcaI 1419 TCATGA T^CATGA
Sau96I 73 GGACC G^G[ACGT]CC
SfuI 2573 TTCGAA TT^CGAA
SgrAI 1756 CACCGGCG C[GA]^CCGG[CT]G
SspBI 1752 TGTACA T^GTACA
StuI 863 AGGCCT AGG^CCT
StyI 42 CCTTGG C^C[AT][AT]GG
XbaI 957 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 14 enzymes) #
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BanI 286 GGTGCC G^G[CT][GA]CC
2226 GGTGCC
BclI 549 TGATCA T^GATCA
1716 TGATCA
BglII 573 AGATCT A^GATCT
952 AGATCT
BsiYI 1580 CCGATGATGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
2271 CCGAGTTTCGG
BslI 1580 CCGATGATGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
2271 CCGAGTTTCGG
FbaI 549 TGATCA T^GATCA
1716 TGATCA
HapII 1227 CCGG C^CGG
1758 CCGG
HpaII 1227 CCGG C^CGG
1758 CCGG
MspA1I 965 CAGCTG C[AC]G^C[GT]G
1319 CAGCTG
MspI 1227 CCGG C^CGG
1758 CCGG
PvuII 965 CAGCTG CAG^CTG
1319 CAGCTG
SfcI 1616 CTACAG C^T[GA][CT]AG
2031 CTACAG
TaqI 2351 TCGA T^CGA
2574 TCGA
TthHB8I 2351 TCGA T^CGA
2574 TCGA
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# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
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AcsI AfaI AluI AlwI AlwNI
ApoI AseI AsnI Asp700I BbvI
BfaI BpmI BsaJI BsmAI BsmI
BsrI BssKI BstF5I BstNI BstYI
CfoI DdeI DpnII EcoRII EcoT22I
Fnu4HI FokI HaeIII HhaI HinP1I
HincII HindII HinfI HphI ItaI
MaeI MaeIII MboI MboII MflI
MnlI MseI MvaI MwoI NdeI
NdeII NlaIII NlaIV NsiI NspI
PleI PshBI RsaI Sau3AI ScrFI
SfaNI SmlI SspI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI XhoII
XmnI