Restriction map for
pF1KA0682
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# NO CUT ENZYMES ( 90 enzymes) #
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AatII Acc65I AccI AclI AflII
AgeI AhdI AocI Aor51HI ApaI
AscI AseI AsnI Asp718I AspEI
AspI AviII AvrII BamHI BbeI
BbvCI BclI BfaI BfrI BglII
BlnI BsiWI BsmBI Bsp1407I BspDI
BspHI BspLU11I BsrGI BssHII Bst1107I
BstZ17I Bsu36I ClaI DraI Eam1105I
Eco47III Eco81I EcoNI EcoRV EcoT22I
FbaI FseI FspI HindIII HpaI
KasI KpnI KspI MaeI MfeI
MluI MunI NarI NdeI NheI
NotI NsiI PacI PaeR7I PflFI
PinAI PmeI PshAI PshBI Psp1406I
PvuI RcaI SacII SalI ScaI
SexAI SfoI SgrAI SmaI SnaBI
SpeI SphI SspBI SspI SwaI
Tth111I XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AccIII 1201 TCCGGA T^CCGGA
AcsI 934 GAATTC [GA]^AATT[CT]
AcyI 1978 GGCGTC G[GA]^CG[CT]C
AflIII 1421 ACGTGT A^C[GA][CT]GT
ApoI 934 GAATTC [GA]^AATT[CT]
AvaI 1165 CTCGGG C^[CT]CG[GA]G
BalI 959 TGGCCA TGG^CCA
BbrPI 1420 CACGTG CAC^GTG
BcgI 230 GCAAGTCATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 466 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 466 GCTGAGC GC^T[ACGT]AGC
BsaAI 1420 CACGTG [CT]AC^GT[GA]
BsaBI 2092 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1978 GGCGTC G[GA]^CG[CT]C
BseAI 1201 TCCGGA T^CCGGA
BsoBI 1165 CTCGGG C^[CT]CG[GA]G
BspEI 1201 TCCGGA T^CCGGA
BsrBI 2771 CCGCTC CCGCTC(-3/-3)
BsrDI 1022 GCAATG GCAATG(2/0)
BsrFI 492 GCCGGC [GA]^CCGG[CT]
BstBI 965 TTCGAA TT^CGAA
BstEII 2739 GGTGACC G^GT[ACGT]ACC
BstPI 2739 GGTGACC G^GT[ACGT]ACC
CelII 466 GCTGAGC GC^T[ACGT]AGC
Cfr10I 492 GCCGGC [GA]^CCGG[CT]
CpoI 2579 CGGTCCG CG^G[AT]CCG
DrdI 1705 GACAACGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 2739 GGTGACC G^GT[ACGT]ACC
EcoRI 934 GAATTC G^AATTC
HaeII 1076 GGCGCT [GA]GCGC^[CT]
HgaI 1979 GCGTC GACGC(5/10)
Hin1I 1978 GGCGTC G[GA]^CG[CT]C
HincII 365 GTCAAC GT[CT]^[GA]AC
HindII 365 GTCAAC GT[CT]^[GA]AC
MamI 2092 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 959 TGGCCA TGG^CCA
MroI 1201 TCCGGA T^CCGGA
MscI 959 TGGCCA TGG^CCA
NaeI 492 GCCGGC GCC^GGC
NgoMIV 492 GCCGGC G^CCGGC
NruI 4 TCGCGA TCG^CGA
NspI 273 ACATGC [GA]CATG^[CT]
NspV 965 TTCGAA TT^CGAA
PmaCI 1420 CACGTG CAC^GTG
PmlI 1420 CACGTG CAC^GTG
RsrII 2579 CGGTCCG CG^G[AT]CCG
SfiI 1902 GGCCCGCAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 965 TTCGAA TT^CGAA
SmlI 2371 CTCAAG C^T[CT][GA]AG
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# TWO CUT ENZYMES ( 19 enzymes) #
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Alw44I 700 GTGCAC G^TGCAC
1124 GTGCAC
ApaLI 700 GTGCAC G^TGCAC
1124 GTGCAC
Asp700I 177 GAAGCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1257 GAAGCTCTTC
BanI 942 GGCACC G^G[CT][GA]CC
1789 GGCACC
BsaWI 822 ACCGGA [AT]^CCGG[AT]
1201 TCCGGA
BsgI 601 GTGCAG GTGCAG(16/14)
779 GTGCAG
BsmI 1563 GAATGC GAATGC(1/-1)
1336 GCATTC
BssSI 1624 CACGAG CACGAG(-5/-1)
2782 CACGAG
BstAPI 792 GCAAGGGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2748 GCAGGCCCTGC
BstXI 1463 CCAGTGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2495 CCAAGATCCTGG
MseI 332 TTAA T^TAA
2201 TTAA
NcoI 2036 CCATGG C^CATGG
2318 CCATGG
SacI 1288 GAGCTC GAGCT^C
2548 GAGCTC
SbfI 756 CCTGCAGG CCTGCA^GG
2745 CCTGCAGG
Sse8387I 756 CCTGCAGG CCTGCA^GG
2745 CCTGCAGG
StuI 1373 AGGCCT AGG^CCT
1910 AGGCCT
Tru9I 332 TTAA T^TAA
2201 TTAA
XcmI 1631 CCAAGGGCAGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1868 CCACTGCCATCGTGG
XmnI 177 GAAGCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1257 GAAGCTCTTC
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# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AspHI AvaII BanII BbsI
BbvI BciVI BcnI BglI BmyI
BpmI BpuAI BsaI BsaJI BseRI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DraIII DsaI EaeI EagI EarI
EclXI Eco52I EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeII MaeIII MboI
MboII MflI MnlI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PflMI PleI
PpuMI PstI PvuII RsaI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TaqI TfiI TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoII