Restriction map for
pF1KA0611
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# NO CUT ENZYMES ( 85 enzymes) #
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AclI AcsI AflII AflIII AgeI
AhdI Aor51HI ApoI AscI AseI
AsnI AspEI AspI AviII AvrII
BamHI BbeI BbrPI BcgI BfaI
BfrI BlnI BsiWI BsmBI BspLU11I
BssHII BstAPI BstXI CpoI DraI
Eam1105I Eco47III EcoRI EcoRV EcoT14I
EcoT22I FseI FspI HgaI HindIII
HpaI KasI MaeI MfeI MluI
MunI NarI NcoI NdeI NheI
NotI NruI NsiI NspI PacI
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RsrII SalI SapI SbfI SexAI
SfiI SfoI SgrAI SnaBI SpeI
SphI Sse8387I SspI StuI StyI
SwaI Tth111I Van91I XbaI XcmI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AatII 1957 GACGTC GACGT^C
Acc65I 575 GGTACC G^GTACC
AccIII 431 TCCGGA T^CCGGA
AcyI 1957 GACGTC G[GA]^CG[CT]C
AlwNI 1186 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1587 CCTGAGG CC^T[ACGT]AGG
ApaI 912 GGGCCC GGGCC^C
Asp700I 1740 GAACGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 575 GGTACC G^GTACC
BclI 1029 TGATCA T^GATCA
BglII 1211 AGATCT A^GATCT
BlpI 2547 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2547 GCTCAGC GC^T[ACGT]AGC
BsaBI 2379 GATTAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1957 GACGTC G[GA]^CG[CT]C
BsaWI 431 TCCGGA [AT]^CCGG[AT]
BseAI 431 TCCGGA T^CCGGA
Bsp1407I 662 TGTACA T^GTACA
BspDI 2596 ATCGAT AT^CGAT
BspEI 431 TCCGGA T^CCGGA
BsrBI 1473 CCGCTC CCGCTC(-3/-3)
BsrDI 1952 GCAATG GCAATG(2/0)
BsrGI 662 TGTACA T^GTACA
Bst1107I 1072 GTATAC GTA^TAC
BstBI 677 TTCGAA TT^CGAA
BstZ17I 1072 GTATAC GTA^TAC
Bsu36I 1587 CCTGAGG CC^T[ACGT]AGG
CelII 2547 GCTCAGC GC^T[ACGT]AGC
ClaI 2596 ATCGAT AT^CGAT
Eco81I 1587 CCTGAGG CC^T[ACGT]AGG
FbaI 1029 TGATCA T^GATCA
Hin1I 1957 GACGTC G[GA]^CG[CT]C
HincII 355 GTCAAC GT[CT]^[GA]AC
HindII 355 GTCAAC GT[CT]^[GA]AC
KpnI 575 GGTACC GGTAC^C
KspI 1716 CCGCGG CCGC^GG
MamI 2379 GATTAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 431 TCCGGA T^CCGGA
NaeI 374 GCCGGC GCC^GGC
NgoMIV 374 GCCGGC G^CCGGC
NspV 677 TTCGAA TT^CGAA
PaeR7I 1732 CTCGAG C^TCGAG
SacI 2314 GAGCTC GAGCT^C
SacII 1716 CCGCGG CCGC^GG
ScaI 1808 AGTACT AGT^ACT
SfuI 677 TTCGAA TT^CGAA
SmaI 697 CCCGGG CCC^GGG
SspBI 662 TGTACA T^GTACA
XhoI 1732 CTCGAG C^TCGAG
XmaCI 697 CCCGGG C^CCGGG
XmaI 697 CCCGGG C^CCGGG
XmnI 1740 GAACGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 26 enzymes) #
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AccI 367 GTCTAC GT^[AC][GT]AC
1072 GTATAC
BalI 1487 TGGCCA TGG^CCA
2453 TGGCCA
BanII 912 GGGCCC G[GA]GC[CT]^C
2314 GAGCTC
BciVI 111 GTATCC GTATCC(6/5)
2307 GTATCC
BglI 967 GCCGTGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1532 GCCTGACGGGC
BsaAI 337 TACGTG [CT]AC^GT[GA]
1644 TACGTG
BsgI 1324 GTGCAG GTGCAG(16/14)
1934 GTGCAG
BsmI 1593 GAATGC GAATGC(1/-1)
1659 GAATGC
BspHI 440 TCATGA T^CATGA
2528 TCATGA
BspMI 578 ACCTGC ACCTGC(4/8)
2687 ACCTGC
BstEII 1671 GGTGACC G^GT[ACGT]ACC
1707 GGTGACC
BstPI 1671 GGTGACC G^GT[ACGT]ACC
1707 GGTGACC
DraIII 991 CACAACGTG CAC[ACGT][ACGT][ACGT]^GTG
2446 CACATCGTG
DrdI 346 GACAAGGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2275 GACAAAGATGTC
EagI 1850 CGGCCG C^GGCCG
2341 CGGCCG
EarI 96 GAAGAG CTCTTC(1/4)
1348 GAAGAG
EclXI 1850 CGGCCG C^GGCCG
2341 CGGCCG
Eco52I 1850 CGGCCG C^GGCCG
2341 CGGCCG
EcoNI 1745 CCTTCCGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1902 CCTGCTTCAGG
EcoO65I 1671 GGTGACC G^GT[ACGT]ACC
1707 GGTGACC
HaeII 2415 GGCGCT [GA]GCGC^[CT]
2499 GGCGCT
Ksp632I 96 GAAGAG CTCTTC(1/4)
1348 GAAGAG
MluNI 1487 TGGCCA TGG^CCA
2453 TGGCCA
MscI 1487 TGGCCA TGG^CCA
2453 TGGCCA
PpuMI 893 AGGACCC [GA]G^G[AT]CC[CT]
1171 AGGACCC
RcaI 440 TCATGA T^CATGA
2528 TCATGA
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# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
#########################################
AccII AciI AfaI AluI Alw44I
AlwI ApaLI AspHI AvaI AvaII
BanI BbsI BbvCI BbvI BcnI
BmyI BpmI BpuAI BsaI BsaJI
BseRI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrFI
BsrI BssKI BssSI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EcoO109I EcoRII FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PleI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII