Restriction map for
pF1KA0609
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# NO CUT ENZYMES ( 106 enzymes) #
##################################
AccI AccIII AclI AflII AgeI
AhdI Alw44I ApaLI AscI AseI
AsnI AspEI AspI AvaI AvrII
BbeI BbrPI BbvCI BcgI BclI
BfaI BfrI BglII BlnI BsaAI
BsaBI BsaI BseAI BsiWI BsmI
BsoBI BspDI BspEI BspHI BssHII
BssSI Bst1107I BstAPI BstEII BstPI
BstZ17I ClaI DraI DrdI EagI
Eam1105I EclXI Eco52I EcoO65I EcoRI
EcoT22I FbaI FseI HincII HindII
HpaI KasI KspI MaeI MamI
MfeI MluI MroI MseI MunI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
SacII SalI SbfI ScaI SexAI
SfiI SfoI SgrAI SmaI SnaBI
SpeI Sse8387I SspI StuI SwaI
Tru9I Tth111I XbaI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AatII 1261 GACGTC GACGT^C
AcsI 28 AAATTC [GA]^AATT[CT]
AocI 1569 CCTCAGG CC^T[ACGT]AGG
Aor51HI 1190 AGCGCT AGC^GCT
ApaI 1333 GGGCCC GGGCC^C
ApoI 28 AAATTC [GA]^AATT[CT]
Asp700I 2038 GAAGAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 629 GGATCC G^GATCC
BbsI 50 GTCTTC GAAGAC(2/6)
BciVI 436 GGATAC GTATCC(6/5)
BglI 2063 GCCGCTACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 50 GTCTTC GAAGAC(2/6)
BsaWI 1856 ACCGGA [AT]^CCGG[AT]
Bsp1407I 1445 TGTACA T^GTACA
BspMI 1475 ACCTGC ACCTGC(4/8)
BsrBI 1092 CCGCTC CCGCTC(-3/-3)
BsrGI 1445 TGTACA T^GTACA
BstBI 100 TTCGAA TT^CGAA
BstXI 1337 CCATCAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1569 CCTCAGG CC^T[ACGT]AGG
CpoI 1771 CGGACCG CG^G[AT]CCG
Eco47III 1190 AGCGCT AGC^GCT
Eco81I 1569 CCTCAGG CC^T[ACGT]AGG
EcoNI 252 CCTGGCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 730 GATATC GAT^ATC
HaeII 1190 AGCGCT [GA]GCGC^[CT]
HgaI 1363 GACGC GACGC(5/10)
HindIII 670 AAGCTT A^AGCTT
NspV 100 TTCGAA TT^CGAA
PpuMI 1702 GGGACCC [GA]G^G[AT]CC[CT]
RsrII 1771 CGGACCG CG^G[AT]CCG
SapI 552 GCTCTTC GCTCTTC(1/4)
SfuI 100 TTCGAA TT^CGAA
SspBI 1445 TGTACA T^GTACA
TfiI 8 GAATC G^A[AT]TC
Tsp509I 29 AATT ^AATT
XcmI 64 CCAGCCATCACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 2038 GAAGAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
Acc65I 827 GGTACC G^GTACC
1721 GGTACC
AcyI 1261 GACGTC G[GA]^CG[CT]C
1363 GACGCC
AlwNI 538 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG
1300 CAGATGCTG
Asp718I 827 GGTACC G^GTACC
1721 GGTACC
AspHI 607 GAGCTC G[AT]GC[AT]^C
967 GAGCTC
AvaII 1703 GGACC G^G[AT]CC
1772 GGACC
AviII 200 TGCGCA TGC^GCA
1481 TGCGCA
BlpI 246 GCTCAGC GC^T[ACGT]AGC
1276 GCTCAGC
Bpu1102I 246 GCTCAGC GC^T[ACGT]AGC
1276 GCTCAGC
BsaHI 1261 GACGTC G[GA]^CG[CT]C
1363 GACGCC
BsiEI 389 CGGTCG CG[GA][CT]^CG
1854 CGACCG
BsiHKAI 607 GAGCTC G[AT]GC[AT]^C
967 GAGCTC
BspLU11I 1517 ACATGT A^CATGT
2054 ACATGT
BsrFI 387 GCCGGT [GA]^CCGG[CT]
1649 ACCGGC
BstYI 539 AGATCC [GA]^GATC[CT]
629 GGATCC
CelII 246 GCTCAGC GC^T[ACGT]AGC
1276 GCTCAGC
Cfr10I 387 GCCGGT [GA]^CCGG[CT]
1649 ACCGGC
DraII 1332 GGGGCCC [GA]G^G[ACGT]CC[CT]
1702 GGGACCC
DraIII 1423 CACAACGTG CAC[ACGT][ACGT][ACGT]^GTG
1438 CACATCGTG
EcoO109I 1332 GGGGCCC [GA]G^G[ACGT]CC[CT]
1702 GGGACCC
FspI 200 TGCGCA TGC^GCA
1481 TGCGCA
Hin1I 1261 GACGTC G[GA]^CG[CT]C
1363 GACGCC
KpnI 827 GGTACC GGTAC^C
1721 GGTACC
MflI 539 AGATCC [GA]^GATC[CT]
629 GGATCC
MslI 888 CACAGGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
972 CATGGGCATG
PstI 12 CTGCAG CTGCA^G
1244 CTGCAG
SacI 607 GAGCTC GAGCT^C
967 GAGCTC
SmlI 610 CTCAAG C^T[CT][GA]AG
2032 CTCAAG
SphI 203 GCATGC GCATG^C
977 GCATGC
XhoII 539 AGATCC [GA]^GATC[CT]
629 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 84 enzymes) #
#########################################
AccII AciI AfaI AflIII AluI
AlwI BalI BanI BanII BbvI
BcnI BmyI BpmI BsaJI BseRI
BsgI BsiYI BslI BsmAI BsmBI
BsmFI Bsp1286I BsrDI BsrI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr13I DdeI DpnII DsaI EaeI
EarI EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeII MaeIII MboI MboII MluNI
MnlI MscI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PflMI PleI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TaqI TseI
Tsp45I TspRI TthHB8I Van91I