Restriction map for
pF1KA0595
##################################
# NO CUT ENZYMES ( 70 enzymes) #
##################################
AatII AclI AcyI AflII AhdI
Aor51HI AseI AsnI AspEI AviII
AvrII BamHI BbeI BbrPI BfrI
BlnI BsaHI BsiEI BsiWI Bsp1407I
BspDI BspHI BsrDI BsrGI Bst1107I
BstBI BstZ17I ClaI DraI EagI
Eam1105I EclXI Eco47III Eco52I EcoRV
FseI FspI HgaI Hin1I HpaI
KasI MfeI MluI MunI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI SfoI SfuI SgrAI
SnaBI SspBI SspI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
Acc65I 2212 GGTACC G^GTACC
AflIII 2926 ACATGT A^C[GA][CT]GT
AgeI 3644 ACCGGT A^CCGGT
Alw44I 4713 GTGCAC G^TGCAC
ApaLI 4713 GTGCAC G^TGCAC
AscI 6 GGCGCGCC GG^CGCGCC
Asp700I 1516 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2212 GGTACC G^GTACC
AspI 25 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BcgI 4216 CGAAAAGCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 809 GTATCC GTATCC(6/5)
BglII 398 AGATCT A^GATCT
BlpI 1879 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1879 GCTGAGC GC^T[ACGT]AGC
BsaAI 3446 CACGTA [CT]AC^GT[GA]
BsaBI 4511 GATCCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 23 GAGACG CGTCTC(1/5)
BsmI 443 GCATTC GAATGC(1/-1)
BspLU11I 2926 ACATGT A^CATGT
BssHII 7 GCGCGC G^CGCGC
BssSI 2128 CTCGTG CACGAG(-5/-1)
BstEII 1212 GGTAACC G^GT[ACGT]ACC
BstPI 1212 GGTAACC G^GT[ACGT]ACC
CelII 1879 GCTGAGC GC^T[ACGT]AGC
CpoI 57 CGGTCCG CG^G[AT]CCG
DraIII 3202 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 312 CCTCATTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1212 GGTAACC G^GT[ACGT]ACC
EcoRI 1599 GAATTC G^AATTC
HindIII 661 AAGCTT A^AGCTT
KpnI 2212 GGTACC GGTAC^C
KspI 81 CCGCGG CCGC^GG
MamI 4511 GATCCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 2057 TTAA T^TAA
MslI 4723 CACTTCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PflFI 25 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 883 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 3644 ACCGGT A^CCGGT
RsrII 57 CGGTCCG CG^G[AT]CCG
SacII 81 CCGCGG CCGC^GG
SalI 1729 GTCGAC G^TCGAC
ScaI 2724 AGTACT AGT^ACT
SexAI 1350 ACCAGGT A^CC[AT]GGT
SfiI 4261 GGCCGCCGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SpeI 1147 ACTAGT A^CTAGT
SphI 2660 GCATGC GCATG^C
StuI 4136 AGGCCT AGG^CCT
Tru9I 2057 TTAA T^TAA
Tth111I 25 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 883 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1662 TCTAGA T^CTAGA
XmnI 1516 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AccIII 60 TCCGGA T^CCGGA
3899 TCCGGA
AcsI 1599 GAATTC [GA]^AATT[CT]
4933 AAATTT
ApoI 1599 GAATTC [GA]^AATT[CT]
4933 AAATTT
BclI 1892 TGATCA T^GATCA
4062 TGATCA
BseAI 60 TCCGGA T^CCGGA
3899 TCCGGA
BspEI 60 TCCGGA T^CCGGA
3899 TCCGGA
BsrBI 4503 CCGCTC CCGCTC(-3/-3)
138 GAGCGG
DrdI 1634 GACAAAGTAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3889 GACTATTGTGTC
EcoT22I 826 ATGCAT ATGCA^T
4035 ATGCAT
FbaI 1892 TGATCA T^GATCA
4062 TGATCA
MroI 60 TCCGGA T^CCGGA
3899 TCCGGA
NcoI 3132 CCATGG C^CATGG
3849 CCATGG
NsiI 826 ATGCAT ATGCA^T
4035 ATGCAT
SacI 1364 GAGCTC GAGCT^C
3137 GAGCTC
SapI 1386 GAAGAGC GCTCTTC(1/4)
4436 GCTCTTC
SbfI 2644 CCTGCAGG CCTGCA^GG
4223 CCTGCAGG
Sse8387I 2644 CCTGCAGG CCTGCA^GG
4223 CCTGCAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AccI AccII AciI AfaI AluI
AlwI AlwNI AocI ApaI AspHI
AvaI AvaII BalI BanI BanII
BbsI BbvCI BbvI BcnI BfaI
BglI BmyI BpmI BpuAI BsaI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrFI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Bsu36I Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI SmlI StyI TaqI TfiI
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoII XmaCI XmaI