Restriction map for
pF1KA0590
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# NO CUT ENZYMES ( 64 enzymes) #
##################################
AccI AflII AgeI AscI AseI
AsnI AvrII BamHI BbeI BbrPI
BciVI BclI BfrI BglII BlnI
BsaBI BsiWI BspDI Bst1107I BstYI
BstZ17I ClaI CpoI EcoRI EcoRV
FbaI FseI HincII HindII HpaI
KasI MamI MfeI MflI MluI
MunI NarI NcoI NdeI NheI
NotI PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI PshBI RsrII
SalI SbfI ScaI SfoI SgrAI
SmlI SnaBI SpeI Sse8387I SspI
SwaI XbaI XhoI XhoII
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# ONE CUT ENZYMES ( 46 enzymes) #
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Acc65I 1024 GGTACC G^GTACC
AccIII 3682 TCCGGA T^CCGGA
AclI 1477 AACGTT AA^CGTT
AcsI 1593 GAATTT [GA]^AATT[CT]
AhdI 2821 GACCTGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 2079 GATCC GGATC(4/5)
Aor51HI 2502 AGCGCT AGC^GCT
ApoI 1593 GAATTT [GA]^AATT[CT]
Asp718I 1024 GGTACC G^GTACC
AspEI 2821 GACCTGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1869 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbvCI 1209 CCTCAGC CCTCAGC(-5/-2)
BfaI 2533 CTAG C^TAG
BsaWI 3682 TCCGGA [AT]^CCGG[AT]
BseAI 3682 TCCGGA T^CCGGA
BsmI 3120 GAATGC GAATGC(1/-1)
BspEI 3682 TCCGGA T^CCGGA
BstBI 1511 TTCGAA TT^CGAA
BstEII 47 GGTCACC G^GT[ACGT]ACC
BstPI 47 GGTCACC G^GT[ACGT]ACC
DraI 1627 TTTAAA TTT^AAA
DrdI 3244 GACAGGGCGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 831 CGGCCG C^GGCCG
Eam1105I 2821 GACCTGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 831 CGGCCG C^GGCCG
Eco47III 2502 AGCGCT AGC^GCT
Eco52I 831 CGGCCG C^GGCCG
EcoO65I 47 GGTCACC G^GT[ACGT]ACC
EcoT22I 1465 ATGCAT ATGCA^T
HindIII 1632 AAGCTT A^AGCTT
KpnI 1024 GGTACC GGTAC^C
KspI 2490 CCGCGG CCGC^GG
MaeI 2533 CTAG C^TAG
MroI 3682 TCCGGA T^CCGGA
NaeI 3643 GCCGGC GCC^GGC
NgoMIV 3643 GCCGGC G^CCGGC
NruI 3710 TCGCGA TCG^CGA
NsiI 1465 ATGCAT ATGCA^T
NspV 1511 TTCGAA TT^CGAA
PflFI 1869 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Psp1406I 1477 AACGTT AA^CGTT
PvuI 2685 CGATCG CGAT^CG
SacII 2490 CCGCGG CCGC^GG
SexAI 494 ACCTGGT A^CC[AT]GGT
SfuI 1511 TTCGAA TT^CGAA
Tth111I 1869 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 21 enzymes) #
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AatII 3357 GACGTC GACGT^C
4129 GACGTC
AocI 781 CCTGAGG CC^T[ACGT]AGG
3550 CCTGAGG
BlpI 816 GCTGAGC GC^T[ACGT]AGC
3180 GCTGAGC
Bpu1102I 816 GCTGAGC GC^T[ACGT]AGC
3180 GCTGAGC
BsiEI 831 CGGCCG CG[GA][CT]^CG
2685 CGATCG
Bsp1407I 767 TGTACA T^GTACA
2594 TGTACA
BspLU11I 545 ACATGT A^CATGT
4229 ACATGT
BsrDI 1462 GCAATG GCAATG(2/0)
2984 GCAATG
BsrGI 767 TGTACA T^GTACA
2594 TGTACA
BssHII 2052 GCGCGC G^CGCGC
2422 GCGCGC
Bsu36I 781 CCTGAGG CC^T[ACGT]AGG
3550 CCTGAGG
CelII 816 GCTGAGC GC^T[ACGT]AGC
3180 GCTGAGC
DraIII 643 CACTATGTG CAC[ACGT][ACGT][ACGT]^GTG
4153 CACTACGTG
Eco81I 781 CCTGAGG CC^T[ACGT]AGG
3550 CCTGAGG
PflMI 1242 CCAGCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2442 CCAGCGGCTGG
SapI 561 GAAGAGC GCTCTTC(1/4)
2101 GAAGAGC
SfiI 2538 GGCCCGCGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
2547 GGCCGTGCTGGCC
SphI 2570 GCATGC GCATG^C
3596 GCATGC
SspBI 767 TGTACA T^GTACA
2594 TGTACA
StuI 3110 AGGCCT AGG^CCT
3926 AGGCCT
Van91I 1242 CCAGCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2442 CCAGCGGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 127 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI Alw44I AlwNI ApaI ApaLI
Asp700I AspHI AvaI AvaII AviII
BalI BanI BanII BbsI BbvI
BcgI BcnI BglI BmyI BpmI
BpuAI BsaAI BsaHI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BspHI BspMI BsrBI BsrFI BsrI
BssKI BssSI BstAPI BstF5I BstNI
BstUI BstXI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI FspI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeII MaeIII MboI
MboII MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PpuMI PstI PvuII
RcaI RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XmaCI
XmaI XmnI