Restriction map for
pF1KA0579
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# NO CUT ENZYMES ( 71 enzymes) #
##################################
AatII AccI AclI AflII AgeI
AhdI AseI AsnI AspEI AviII
AvrII BamHI BbeI BcgI BclI
BfrI BlnI BsaBI BsiWI BspDI
BspHI BspLU11I BssSI Bst1107I BstBI
BstZ17I ClaI DraI DraIII DrdI
Eam1105I EcoNI EcoRV FbaI FseI
FspI HincII HindII HindIII HpaI
KasI KspI MamI MfeI MluI
MunI NarI NdeI NheI NotI
NruI NspV PacI PinAI PmeI
PshBI Psp1406I PvuI RcaI SacII
SalI SapI SexAI SfiI SfoI
SfuI SgrAI SnaBI SpeI SspI
SwaI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AccIII 486 TCCGGA T^CCGGA
AcyI 1872 GACGCC G[GA]^CG[CT]C
Aor51HI 1312 AGCGCT AGC^GCT
AscI 109 GGCGCGCC GG^CGCGCC
Asp700I 1386 GAATCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 2159 TGGCCA TGG^CCA
BbvCI 1315 GCTGAGG CCTCAGC(-5/-2)
BciVI 710 GTATCC GTATCC(6/5)
BglII 993 AGATCT A^GATCT
BsaHI 1872 GACGCC G[GA]^CG[CT]C
BsaI 1849 GAGACC GGTCTC(1/5)
BseAI 486 TCCGGA T^CCGGA
BsmBI 1077 GAGACG CGTCTC(1/5)
BsmI 210 GAATGC GAATGC(1/-1)
Bsp1407I 2027 TGTACA T^GTACA
BspEI 486 TCCGGA T^CCGGA
BsrGI 2027 TGTACA T^GTACA
BstXI 1271 CCAGCCCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CpoI 2445 CGGTCCG CG^G[AT]CCG
Eco47III 1312 AGCGCT AGC^GCT
EcoRI 1530 GAATTC G^AATTC
EcoT22I 1793 ATGCAT ATGCA^T
Hin1I 1872 GACGCC G[GA]^CG[CT]C
MluNI 2159 TGGCCA TGG^CCA
MroI 486 TCCGGA T^CCGGA
MscI 2159 TGGCCA TGG^CCA
MseI 950 TTAA T^TAA
NaeI 757 GCCGGC GCC^GGC
NgoMIV 757 GCCGGC G^CCGGC
NsiI 1793 ATGCAT ATGCA^T
PshAI 1779 GACCAAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsrII 2445 CGGTCCG CG^G[AT]CCG
SbfI 2085 CCTGCAGG CCTGCA^GG
ScaI 1253 AGTACT AGT^ACT
Sse8387I 2085 CCTGCAGG CCTGCA^GG
SspBI 2027 TGTACA T^GTACA
Tru9I 950 TTAA T^TAA
XmnI 1386 GAATCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 42 enzymes) #
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Acc65I 539 GGTACC G^GTACC
664 GGTACC
AflIII 563 ACGTGT A^C[GA][CT]GT
1332 ACGTGT
AocI 263 CCTTAGG CC^T[ACGT]AGG
534 CCTGAGG
ApaI 1156 GGGCCC GGGCC^C
2079 GGGCCC
Asp718I 539 GGTACC G^GTACC
664 GGTACC
AspI 577 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
2581 GACCCTGTC
BbrPI 514 CACGTG CAC^GTG
562 CACGTG
BbsI 1818 GAAGAC GAAGAC(2/6)
1581 GTCTTC
BfaI 904 CTAG C^TAG
1606 CTAG
BpuAI 1818 GAAGAC GAAGAC(2/6)
1581 GTCTTC
BsaAI 514 CACGTG [CT]AC^GT[GA]
562 CACGTG
BsiEI 755 CGGCCG CG[GA][CT]^CG
1859 CGGCCG
BsrBI 726 GAGCGG CCGCTC(-3/-3)
2706 GAGCGG
BsrDI 2059 GCAATG GCAATG(2/0)
2684 GCAATG
BssHII 110 GCGCGC G^CGCGC
128 GCGCGC
BstAPI 39 GCAGGTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2354 GCATTGTGTGC
BstEII 1110 GGTCACC G^GT[ACGT]ACC
2743 GGTCACC
BstPI 1110 GGTCACC G^GT[ACGT]ACC
2743 GGTCACC
Bsu36I 263 CCTTAGG CC^T[ACGT]AGG
534 CCTGAGG
EagI 755 CGGCCG C^GGCCG
1859 CGGCCG
EarI 448 GAAGAG CTCTTC(1/4)
1297 GAAGAG
EclXI 755 CGGCCG C^GGCCG
1859 CGGCCG
Eco52I 755 CGGCCG C^GGCCG
1859 CGGCCG
Eco81I 263 CCTTAGG CC^T[ACGT]AGG
534 CCTGAGG
EcoO65I 1110 GGTCACC G^GT[ACGT]ACC
2743 GGTCACC
KpnI 539 GGTACC GGTAC^C
664 GGTACC
Ksp632I 448 GAAGAG CTCTTC(1/4)
1297 GAAGAG
MaeI 904 CTAG C^TAG
1606 CTAG
NspI 1523 GCATGC [GA]CATG^[CT]
1791 GCATGC
PaeR7I 1130 CTCGAG C^TCGAG
2394 CTCGAG
PflFI 577 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
2581 GACCCTGTC
PflMI 631 CCAAGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2664 CCAAGGACTGG
PmaCI 514 CACGTG CAC^GTG
562 CACGTG
PmlI 514 CACGTG CAC^GTG
562 CACGTG
PpuMI 1718 AGGTCCT [GA]G^G[AT]CC[CT]
2572 GGGACCC
SphI 1523 GCATGC GCATG^C
1791 GCATGC
StuI 531 AGGCCT AGG^CCT
688 AGGCCT
Tth111I 577 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
2581 GACCCTGTC
Van91I 631 CCAAGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2664 CCAAGGACTGG
XbaI 903 TCTAGA T^CTAGA
1605 TCTAGA
XcmI 885 CCAGGAGCAGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1115 CCAGCAGTGGTGTGG
XhoI 1130 CTCGAG C^TCGAG
2394 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 107 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
Alw44I AlwI AlwNI ApaLI ApoI
AspHI AvaI AvaII BanI BanII
BbvI BcnI BglI BlpI BmyI
BpmI Bpu1102I BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrFI
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI MaeII MaeIII MboI
MboII MflI MnlI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV PleI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
SmlI StyI TaqI TfiI TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII
XmaCI XmaI